AB27792

Anti-eIF4EBP1 (phospho T46) antibody

Name: Anti-eIF4EBP1 (phospho T46) antibody (ab27792) | Abcam
Brand: Abcam Limited
SKU: AB27792
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal eIF4EBP1 phospho T46 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human EIF4EBP1 phospho T46.

View Alternative Names

Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, EIF4EBP1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T46) antibody (AB27792)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T46) antibody (AB27792)

Immunofluorescence analysis of eIF4EBP1 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with 4EBP1 (pT46) Rabbit Polyclonal Antibody (ab27792) at 1/250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Rhodamine Phalloidin (1/300). Panel d is a merged image showing Nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

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Western blot - Anti-eIF4EBP1 (phospho T46) antibody (AB27792)

The figure shows that ab27792 can detect phosphorylated eIF4EBP1 in PDGF treated NIH3T3 cells (lane 1), but does not detect the unphosphorylated form (lanes 2 and 3 : controls). Blocking step : 3% BSA TBST buffer for one hour at room temperature.

All lanes:

Western blot - Anti-eIF4EBP1 (phospho T46) antibody (ab27792) at 1/1000 dilution

Lane 1:

PDGF treated NIH3T3 cell lysate

Lane 2:

PI3K inhibitor LY2904002 treated NIH3T3 cell lysate

Lane 3:

PI3K inhibitor LY2904002 and PDGF treated NIH3T3 cell lysate

Secondary

All lanes:

goat F(ab)2 anti rabbit IgG HRP conjugate

Predicted band size: 13 kDa

Observed band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human EIF4EBP1 phospho T46. The exact immunogen used to generate this antibody is proprietary information.

Q13541

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Ab27792 was purified from rabbit serum by sequential epitope specific chromatography. The antibody was negatively preadsorbed using a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non phosphorylated eIF4EBP1. The final product is generated by affinity chromatography using a eIF4EBP1 derived peptide that is phosphorylated at threonine 46.

Storage buffer

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4EBP1 also known as 4EBP1 or p4EBP1 is an important protein that acts as a translational repressor by binding to eIF4E therefore inhibiting cap-dependent translation. The molecular weight of 4EBP1 is approximately 12 kDa. This protein is ubiquitously expressed in various tissues indicating its widespread involvement in cellular functions. eIF4EBP1 can be detected and quantified using techniques like the eIF4EBP1 ELISA making it a common target in research for its role in translation regulation.

Biological function summary

EIF4EBP1 plays an important role in regulating cell growth and proliferation by modulating protein synthesis. It is a part of the eIF4F complex which is responsible for the initiation of mRNA translation. When hypophosphorylated eIF4EBP1 binds tightly to eIF4E and prevents the assembly of the active eIF4F complex leading to reduced translation initiation of mRNAs involved in growth and survival. This regulation is important in conditions where cells need to adapt to metabolic stress or external signals.

Pathways

The mammalian target of rapamycin (mTOR) pathway regulates eIF4EBP1 through phosphorylation. In response to growth signals mTOR phosphorylates eIF4EBP1 causing the release of eIF4E and allowing mRNA translation to proceed. This interaction links eIF4EBP1 to the PI3K/AKT/mTOR signaling pathway which influences cell cycle progression and survival. Related proteins in this pathway include ribosomal protein S6 kinase (S6K1) which is also phosphorylated by mTOR to promote protein synthesis.

Deregulation of eIF4EBP1 has been implicated in cancer and neurological disorders. In cancer hyperactive mTOR signaling can lead to excessive phosphorylation of 4EBP1 decreasing its ability to inhibit eIF4E and enhancing translation of oncogenic proteins. In neurological disorders imbalanced eIF4EBP1 activity can disrupt synaptic plasticity and memory formation. Proteins such as p70S6K which are also part of the mTOR pathway share connections with eIF4EBP1 in these pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex : hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

See full target information EIF4EBP1 phospho T46

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 114:855-869 PubMed36403197

2022

TTI1 promotes non-small-cell lung cancer progression by regulating the mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Xian Zhang,Xin Yang,Zhi-Bo Wu,Zhong-Min Liao,Ding-Guo Wang,Shi-Wei Chen,Feng Lu,Yong-Bing Wu,Shu-Qiang Zhu

Mediators of inflammation 2022:6077570 PubMed35915740

2022

mTOR Modulates the Endoplasmic Reticulum Stress-Induced CD4 T Cell Apoptosis Mediated by ROS in Septic Immunosuppression.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wang,Jianwei Chen,Guangxu Bai,Wen Han,Ran Guo,Na Cui

Journal of experimental & clinical cancer research : CR 40:66 PubMed33579337

2021

Circ_0119872 promotes uveal melanoma development by regulating the miR-622/G3BP1 axis and downstream signalling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Shuting Liu,Liang Chen,Hua Chen,Kangkang Xu,Xi Peng,Mingchang Zhang

Nature cell biology 22:1239-1251 PubMed32958857

2020

SMN-primed ribosomes modulate the translation of transcripts related to spinal muscular atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Fabio Lauria,Paola Bernabò,Toma Tebaldi,Ewout Joan Nicolaas Groen,Elena Perenthaler,Federica Maniscalco,Annalisa Rossi,Deborah Donzel,Massimiliano Clamer,Marta Marchioretto,Neža Omersa,Julia Orri,Mauro Dalla Serra,Gregor Anderluh,Alessandro Quattrone,Alberto Inga,Thomas Henry Gillingwater,Gabriella Viero

Journal of gastric cancer 20:95-105 PubMed32269848

2020

The Influence of Bcl-3 Expression on Cell Migration and Chemosensitivity of Gastric Cancer Cells via Regulating Hypoxia-Induced Protective Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Hu,Zhigang Bai,Xuemei Ma,Nan Bai,Zhongtao Zhang

View all publications

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