AB2609

Anti-eIF4G1 antibody

Name: Anti-eIF4G1 antibody (ab2609) | Abcam
Brand: Abcam Limited
SKU: AB2609
Price: 435 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(23 Publications)

Rabbit Polyclonal eIF4G1 antibody. Suitable for IHC-P, IP, ICC/IF, WB and reacts with Human, Rat, African green monkey samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human EIF4G1 aa 550-650.

View Alternative Names

EIF4F, EIF4G, EIF4GI, EIF4G1, Eukaryotic translation initiation factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 antibody (AB2609)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 antibody (AB2609)

ab2609 (4µg/ml) staining eIF4G1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm of the intestinal cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Immunocytochemistry/ Immunofluorescence - Anti-eIF4G1 antibody (AB2609)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-eIF4G1 antibody (AB2609)

ICC/IF image of ab2609 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2609, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Supplier Data

Immunoprecipitation - Anti-eIF4G1 antibody (AB2609)

Lane 1 : immunoprecipitated by ab2609 at 6 μg per reaction; Lane 2 : Immunoprecipitated by control IgG at 6 μg per reaction.

All lanes:

Immunoprecipitation - Anti-eIF4G1 antibody (ab2609) at 1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

HeLa whole cell lysate

Predicted band size: 175 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-eIF4G1 antibody (AB2609)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-eIF4G1 antibody (ab2609) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 175 kDa

false

Exposure time: 3min

Unknown

Western blot - Anti-eIF4G1 antibody (AB2609)

50μg (lane 1) and 150μg (lane 2) rat liver lysate, separated on 7.5% acrylamide SDS-PAGE gel. Detected using ab2609 at 1 : 1000 (A), 1 : 5000 (B) and 1 : 10000 (C) dilution by ECL.

50µg (lane 1) and 150µg (lane 2) rat liver lysate, separated on 7.5% acrylamide SDS-PAGE gel. Detected using ab2609 at 1 : 1000 (A), 1 : 5000 (B) and 1 : 10000 (C) dilution by ECL.

All lanes:

Western blot - Anti-eIF4G1 antibody (ab2609)

Predicted band size: 175 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

African green monkey, Rat, Human

Applications

IP, WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human EIF4G1 aa 550-650. The exact immunogen used to generate this antibody is proprietary information.

Q04637

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4 µg/mL", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/1000", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "EIF4G is more susceptible to degradation compared to other proteins, especially from some tissue sources such as liver. This is true even when tissue is stored at frozen. SDS-PAGE sample buffer may improve the stability, but samples that are stored frozen may show degradation bands that have been described in the literature.Therefore if multiple bands are observed in WB, it is likely due to the degradation of eIF4G rather than non-specificity of the antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "EIF4G is more susceptible to degradation compared to other proteins, especially from some tissue sources such as liver. This is true even when tissue is stored at frozen. SDS-PAGE sample buffer may improve the stability, but samples that are stored frozen may show degradation bands that have been described in the literature.Therefore if multiple bands are observed in WB, it is likely due to the degradation of eIF4G rather than non-specificity of the antibody." }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "4 µg/mL", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/1000", "IP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "EIF4G is more susceptible to degradation compared to other proteins, especially from some tissue sources such as liver. This is true even when tissue is stored at frozen. SDS-PAGE sample buffer may improve the stability, but samples that are stored frozen may show degradation bands that have been described in the literature.Therefore if multiple bands are observed in WB, it is likely due to the degradation of eIF4G rather than non-specificity of the antibody." }, "Cat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification notes

Affinity purified using the immunising peptide immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4G1 also known as eukaryotic translation initiation factor 4 gamma 1 plays an important role in protein synthesis by acting as a scaffold for various other components of the initiation complex. This protein physically binds with eIF4E eIF4A and the poly(A)-binding protein aiding in the recruitment of ribosomes to the mRNA. eIF4G1 has a molecular weight of approximately 180 kDa and is expressed in various tissues including brain heart and skeletal muscle.

Biological function summary

EIF4G1 functions as part of the eIF4F complex facilitating the initiation of cap-dependent translation. This process directly affects the synthesis of proteins that are important for growth and cell cycle progression. As an important component in translation eIF4G1 coordinates interactions between mRNA and ribosomes ensuring efficient translation initiation and progression.

Pathways

EIF4G1 is significant in the mTOR and MAPK signaling pathways. These pathways regulate various cellular processes such as cell growth proliferation and survival. eIF4G1 interacts with related proteins like eIF4E which binds the 5' cap of mRNA and eIF4A an RNA helicase both critical in these signaling cascades.

EIF4G1 has been associated with neurodegenerative diseases like Parkinson's disease and certain types of cancer. Mutations or dysregulation can alter protein synthesis rates and lead to the development of disease phenotypes. In Parkinson's disease the interplay between eIF4G1 and other proteins like alpha-synuclein provides insight into the cellular mechanisms underlying disease progression highlighting eIF4G1 as a potential therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (PubMed : 29987188). Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive) (PubMed : 29987188). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon (PubMed : 29987188). Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning (PubMed : 29987188). As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation (PubMed : 29062139).

See full target information EIF4G1

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 15:e70270 PubMed40088428

2025

Targeting YBX1-m5C mediates RNF115 mRNA circularisation and translation to enhance vulnerability of ferroptosis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ouwen Li,Ke An,Han Wang,Xianbin Li,Yueqin Wang,Lan Huang,Yue Du,Nuo Qin,Jiasheng Dong,Jingyao Wei,Ranran Sun,Yong Shi,Yanjia Guo,Xiangyi Sun,Ying Yang,Yun-Gui Yang,Quancheng Kan,Xin Tian

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2309234 PubMed38380498

2024

Development and Application of the CRISPR-dcas13d-eIF4G Translational Regulatory System to Inhibit Ferroptosis in Calcium Oxalate Crystal-Induced Kidney Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqi He,Chao Song,Sheng Li,Caitao Dong,Wenbiao Liao,Yunhe Xiong,Sixing Yang,Yuchen Liu

Nucleic acids research 51:8820-8835 PubMed37449412

2023

Paralogous translation factors target distinct mRNAs to differentially regulate tolerance to oxidative stress in yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Joanne Cunningham,Aristeidis P Sfakianos,Paraskevi Kritsiligkou,Christopher J Kershaw,Alan J Whitmarsh,Simon J Hubbard,Mark P Ashe,Chris M Grant

JCI insight 7: PubMed36125898

2022

Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein-mediated viral translation.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Jing Shen,Chia-Ling Chen,Tsung-Ting Tsai,Ming-Kai Jhan,Chyi-Huey Bai,Yu-Chun Yen,Ching-Wen Tsai,Cheng-Yi Lee,Po-Chun Tseng,Chia-Yi Yu,Chiou-Feng Lin

International journal of molecular medicine 47: PubMed33693953

2021

gene expression signature analysis revealed bosutinib as a radiosensitizer of breast cancer cells by targeting eIF4G1.

Applications

Unspecified application

Species

Unspecified reactive species

Sai Hu,Dafei Xie,Pingkun Zhou,Xiaodan Liu,Xiaoyao Yin,Bo Huang,Hua Guan

Journal of cellular and molecular medicine 25:2795-2805 PubMed33539648

2021

Role of EIF4G1 network in non-small cell lung cancers (NSCLC) cell survival and disease progression.

Applications

Unspecified application

Species

Unspecified reactive species

Luis Del Valle,Lu Dai,Hui-Yi Lin,Zhen Lin,Jungang Chen,Steven R Post,Zhiqiang Qin

Cancer cell international 20:449 PubMed32943997

2020

Down-regulation of lncRNA UCA1 enhances radiosensitivity in prostate cancer by suppressing EIF4G1 expression via sponging miR-331-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Minhua Hu,Jincheng Yang

Viruses 8: PubMed27447663

2016

N-Terminal Domain of Feline Calicivirus (FCV) Proteinase-Polymerase Contributes to the Inhibition of Host Cell Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Hongxia Wu,Shaopo Zu,Xue Sun,Yongxiang Liu,Jin Tian,Liandong Qu

eLife 4: PubMed26678009

2015

Translational control of nociception via 4E-binding protein 1.

Applications

Species

Unspecified reactive species

Arkady Khoutorsky,Robert P Bonin,Robert E Sorge,Christos G Gkogkas,Sophie Anne Pawlowski,Seyed Mehdi Jafarnejad,Mark H Pitcher,Tommy Alain,Jimena Perez-Sanchez,Eric W Salter,Loren Martin,Alfredo Ribeiro-da-Silva,Yves De Koninck,Fernando Cervero,Jeffrey S Mogil,Nahum Sonenberg

Cancer discovery 5:255-63 PubMed25564516

2015

Lysine demethylase KDM4A associates with translation machinery and regulates protein synthesis.

Applications

Species

Human

Capucine Van Rechem,Joshua C Black,Myriam Boukhali,Martin J Aryee,Susanne Gräslund,Wilhelm Haas,Cyril H Benes,Johnathan R Whetstine

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-eIF4G1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 antibody (AB2609)

Immunocytochemistry/ Immunofluorescence - Anti-eIF4G1 antibody (AB2609)

Immunoprecipitation - Anti-eIF4G1 antibody (AB2609)

Western blot - Anti-eIF4G1 antibody (AB2609)

Western blot - Anti-eIF4G1 antibody (AB2609)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

Product promise