Anti-eIF4G1 antibody

5 product images

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  Western blot - Anti-eIF4G1 antibody (ab2609)

  Lysates prepared using NETN lysis buffer.

  All lanes: Western blot - Anti-eIF4G1 antibody (ab2609) at 0.1 µg/mL

  Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

  Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

  Predicted band size: 175 kDa

  Exposure time: 3min

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  Western blot - Anti-eIF4G1 antibody (ab2609)

  50μg (lane 1) and 150μg (lane 2) rat liver lysate, separated on 7.5% acrylamide SDS-PAGE gel. Detected using ab2609 at 1:1000 (A), 1:5000 (B) and 1:10000 (C) dilution by ECL.

  50µg (lane 1) and 150µg (lane 2) rat liver lysate, separated on 7.5% acrylamide SDS-PAGE gel. Detected using ab2609 at 1:1000 (A), 1:5000 (B) and 1:10000 (C) dilution by ECL.

  All lanes: Western blot - Anti-eIF4G1 antibody (ab2609)

  Predicted band size: 175 kDa

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  Immunoprecipitation - Anti-eIF4G1 antibody (ab2609)

  Lane 1: immunoprecipitated by ab2609 at 6 μg per reaction;
  Lane 2: Immunoprecipitated by control IgG at 6 μg per reaction.

  All lanes: Immunoprecipitation - Anti-eIF4G1 antibody (ab2609) at 1 µg/mL

  Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

  Lane 2: HeLa whole cell lysate

  Predicted band size: 175 kDa

  Exposure time: 10s

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 antibody (ab2609)

  ab2609 (4µg/ml) staining eIF4G1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm of the intestinal cells.
  Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  

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  Immunocytochemistry/ Immunofluorescence - Anti-eIF4G1 antibody (ab2609)

  ICC/IF image of ab2609 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2609, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.