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Rabbit Monoclonal eIF4G1 phospho S1108 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free (AB242419), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free (AB242419), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free (AB242419), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free (AB242419), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free (AB242419), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Primary Antibody Incubation: 10 minutes at room temperature.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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Target data

Function

Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (PubMed:29987188). Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive) (PubMed:29987188). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon (PubMed:29987188). Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning (PubMed:29987188). As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation (PubMed:29062139).

Alternative names

EIF4F, EIF4G, EIF4GI, EIF4G1, Eukaryotic translation initiation factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220

Recommended products

Rabbit Monoclonal eIF4G1 phospho S1108 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
SP351
Purification technique
Affinity purification Protein A/G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab242419 is the carrier-free version of Anti-eIF4G1 (phospho S1108) antibody [SP351] ab223502.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EIF4G1 also known as eukaryotic translation initiation factor 4 gamma 1 plays an important role in protein synthesis by acting as a scaffold for various other components of the initiation complex. This protein physically binds with eIF4E eIF4A and the poly(A)-binding protein aiding in the recruitment of ribosomes to the mRNA. eIF4G1 has a molecular weight of approximately 180 kDa and is expressed in various tissues including brain heart and skeletal muscle.

Biological function summary

EIF4G1 functions as part of the eIF4F complex facilitating the initiation of cap-dependent translation. This process directly affects the synthesis of proteins that are important for growth and cell cycle progression. As an important component in translation eIF4G1 coordinates interactions between mRNA and ribosomes ensuring efficient translation initiation and progression.

Pathways

EIF4G1 is significant in the mTOR and MAPK signaling pathways. These pathways regulate various cellular processes such as cell growth proliferation and survival. eIF4G1 interacts with related proteins like eIF4E which binds the 5' cap of mRNA and eIF4A an RNA helicase both critical in these signaling cascades.

Associated diseases and disorders

EIF4G1 has been associated with neurodegenerative diseases like Parkinson's disease and certain types of cancer. Mutations or dysregulation can alter protein synthesis rates and lead to the development of disease phenotypes. In Parkinson's disease the interplay between eIF4G1 and other proteins like alpha-synuclein provides insight into the cellular mechanisms underlying disease progression highlighting eIF4G1 as a potential therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free (ab242419), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free (ab242419)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling eIF4G1 with Purified Anti-eIF4G1 (phospho S1108) antibody [SP351] ab223502 at 1/100 dilution (1.20 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF4G1 (phospho S1108) antibody [SP351] ab223502)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free (ab242419), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free (ab242419)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling eIF4G1 with Purified Anti-eIF4G1 (phospho S1108) antibody [SP351] ab223502 at 1/100 dilution (1.20 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF4G1 (phospho S1108) antibody [SP351] ab223502)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free (ab242419), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free (ab242419)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for eIF4G1 (phospho S1108) with Anti-eIF4G1 (phospho S1108) antibody [SP351] ab223502 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-eIF4G1 (phospho S1108) antibody [SP351] ab223502).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free (ab242419), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free (ab242419)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for eIF4G1 (phospho S1108) with Anti-eIF4G1 (phospho S1108) antibody [SP351] ab223502 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-eIF4G1 (phospho S1108) antibody [SP351] ab223502).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free (ab242419), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 (phospho S1108) antibody [SP351] - BSA and Azide free (ab242419)

    Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for eIF4G1 (phospho S1108) with Anti-eIF4G1 (phospho S1108) antibody [SP351] ab223502 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-eIF4G1 (phospho S1108) antibody [SP351] ab223502).

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Product protocols

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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