Anti-eIF4GII antibody - N-terminal

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-eIF4GII antibody - N-terminal (AB155482)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling EIF4G3 protein at cytoplasm using ab155482 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).
Scale bar= 10 µm.

• IP

Supplier Data

Immunoprecipitation - Anti-eIF4GII antibody - N-terminal (AB155482)

Immunoprecipitation of EIF4G3 protein from 293T whole cell extracts using 5 µg of ab155482. Western blot analysis was performed using ab155482.

All lanes:

Immunoprecipitation - Anti-eIF4GII antibody - N-terminal (ab155482) at 5 µg

Lane 1:

293T whole cell extracts (Input)

Lane 2:

293T whole cell extracts with control IgG (-)

Lane 3:

293T whole cell extracts (+)

Secondary

All lanes:

Anti-Rabbit IgG

Predicted band size: 177 kDa

false

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4GII antibody - N-terminal (AB155482)

Immunohistochemical analysis of paraffin-embedded mouse brain stem tissue stained for EIF4G3 protein at cytosol with ab155482 at a 1/500 dilution.

Antigen retrieval : EDTA based buffer, (pH 8.0), 15min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4GII antibody - N-terminal (AB155482)

Immunohistochemical analysis of paraffin-embedded rat hind brain tissue stained for EIF4G3 protein at cytosol with ab155482 at a 1/500 dilution.

Antigen retrieval : EDTA based buffer, (pH 8.0), 15min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4GII antibody - N-terminal (AB155482)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue stained for EIF4G3 protein at cytoplasm with ab155482 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• WB

Supplier Data

Western blot - Anti-eIF4GII antibody - N-terminal (AB155482)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-eIF4GII antibody - N-terminal (ab155482) at 1/10000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Predicted band size: 177 kDa

false

• WB

Supplier Data

Western blot - Anti-eIF4GII antibody - N-terminal (AB155482)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-eIF4GII antibody - N-terminal (ab155482) at 1/1000 dilution

All lanes:

Mouse testis tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 177 kDa

false