AB85913

Anti-EIF5 antibody

Name: Anti-EIF5 antibody (ab85913) | Abcam
Brand: Abcam Limited
SKU: AB85913
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal EIF5 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human Eukaryotic translation initiation factor 5 aa 100-150.

View Alternative Names

Eukaryotic translation initiation factor 5, eIF-5, EIF5

1 Images

Unknown

Western blot - Anti-EIF5 antibody (AB85913)

Detection : Chemiluminescence

All lanes:

Western blot - Anti-EIF5 antibody (ab85913) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 49 kDa

Observed band size: 49 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human Eukaryotic translation initiation factor 5 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P55010

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Medaka fish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Tilapia": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab85913 was affinity purified using an epitope specific to EIF5 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic translation initiation factor 5 (EIF5) plays an important role in the initiation phase of protein synthesis. EIF5 also known as eIF-5 is a protein that exhibits a molecular weight of approximately 49 kDa. It functions as a GTPase-activating protein that triggers the hydrolysis of GTP bound to the eukaryotic initiation factor 2 (eIF2) an essential step for the formation of the 80S ribosome complex during translation. EIF5 expression occurs widely across various tissues indicating its necessity for basic cellular functions.

Biological function summary

EIF5 facilitates the translation initiation process which is critical for protein synthesis. It is an integral component of the multifactor eIF2 complex that ensures the accuracy of start codon recognition in mRNA. This complex assembly involves the association with other initiation factors like eIF1 and eIF3 orchestrating mRNA scanning and the selection of the start site for protein synthesis. EIF5's role in regulating protein biosynthesis links it to the maintenance of cellular homeostasis.

Pathways

EIF5 serves a critical part in the translation initiation pathway. This pathway is essential for general protein biosynthesis directly impacting cellular growth and proliferation. It closely collaborates with related factors like eIF2 and eIF2B within this pathway to modulate translation in response to cellular stress and nutrient availability. Additionally EIF5 intersects the mTOR signaling pathway where it interacts with proteins that coordinate cell growth and metabolism under various physiological conditions.

EIF5 alterations can lead to disruptions in protein synthesis connecting it to certain human diseases like cancer. Some studies indicate a link between EIF5 dysregulation and tumorigenesis as faults in translation regulation can drive abnormal cell proliferation. Beyond cancer EIF5 may also intersect with neurodegenerative diseases given the translation regulation's importance in neuronal function. It can associate with other factors such as eIF2α wherein heightened EIF5 activity influences stress responses pivotal in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon (PubMed : 11166181, PubMed : 22813744, PubMed : 24319994). In this complex, acts as a GTPase-activating protein, by promoting GTP hydrolysis by eIF2G (EIF2S3) (PubMed : 11166181). During scanning, interacts with both EIF1 (via its C-terminal domain (CTD)) and EIF1A (via its NTD) (PubMed : 22813744). This interaction with EIF1A contributes to the maintenance of EIF1 within the open 43S PIC (PubMed : 24319994). When start codon is recognized, EIF5, via its NTD, induces eIF2G (EIF2S3) to hydrolyze the GTP (PubMed : 11166181). Start codon recognition also induces a conformational change of the PIC to a closed state (PubMed : 22813744). This change increases the affinity of EIF5-CTD for EIF2-beta (EIF2S2), which allows the release, by an indirect mechanism, of EIF1 from the PIC (PubMed : 22813744). Finally, EIF5 stabilizes the PIC in its closed conformation (PubMed : 22813744).

See full target information Eukaryotic translation initiation factor 5

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PloS one 13:e0192648 PubMed29470543

2018

Translational autoregulation of BZW1 and BZW2 expression by modulating the stringency of start codon selection.

Applications

Unspecified application

Species

Unspecified reactive species

Gary Loughran,Andrew E Firth,John F Atkins,Ivaylo P Ivanov

Open biology 6: PubMed27249819

2016

Systematic analysis of the PTEN 5' leader identifies a major AUU initiated proteoform.

Applications

Unspecified application

Species

Unspecified reactive species

Ioanna Tzani,Ivaylo P Ivanov,Dmitri E Andreev,Ruslan I Dmitriev,Kellie A Dean,Pavel V Baranov,John F Atkins,Gary Loughran

Journal of cutaneous pathology 40:651-6 PubMed23521708

2013

Levels of the EMT-related protein Snail/Slug are not correlated with p53/p63 in cutaneous squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hongxiang Chen,Masakazu Takahara,Lining Xie,Satoshi Takeuchi,Yating Tu,Takeshi Nakahara,Hiroshi Uchi,Yoichi Moroi,Masutaka Furue

Nucleic acids research 40:2898-906 PubMed22156057

2011

Stringency of start codon selection modulates autoregulation of translation initiation factor eIF5.

Applications

Species

Human

Gary Loughran,Matthew S Sachs,John F Atkins,Ivaylo P Ivanov

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-EIF5 antibody

Western blot - Anti-EIF5 antibody (AB85913)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise