Rabbit Polyclonal EIF5 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human Eukaryotic translation initiation factor 5 aa 100-150.
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Publications
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- PloS one 13:e01926482018Translational autoregulation of BZW1 and BZW2 expression by modulating the stringency of start codon selection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGary Loughran et. al.PubMed 29470543
- Open biology 6:2016Systematic analysis of the PTEN 5' leader identifies a major AUU initiated proteoform.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIoanna Tzani,Ivaylo P Ivanov,Dmitri E Andreev,Ruslan I Dmitriev,Kellie A Dean,Pavel V Baranov,John F Atkins,Gary LoughranPubMed 27249819
- Journal of cutaneous pathology 40:651-62013Levels of the EMT-related protein Snail/Slug are not correlated with p53/p63 in cutaneous squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongxiang Chen,Masakazu Takahara,Lining Xie,Satoshi Takeuchi,Yating Tu,Takeshi Nakahara,Hiroshi Uchi,Yoichi Moroi,Masutaka FuruePubMed 23521708
- Nucleic acids research 40:2898-9062011Stringency of start codon selection modulates autoregulation of translation initiation factor eIF5.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Eukaryotic translation initiation factor 5 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link P55010
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Predicted |
| Chicken | Predicted |
| Chimpanzee | Predicted |
| Cow | Predicted |
| Guinea pig | Predicted |
| Horse | Predicted |
| Medaka fish | Predicted |
| Orangutan | Predicted |
| Rabbit | Predicted |
| Rhesus monkey | Predicted |
| Tilapia | Predicted |
| Turkey | Predicted |
| Xenopus laevis | Predicted |
| Xenopus tropicalis | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Xenopus laevis, Chimpanzee, Rhesus monkey, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish, Rat | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon (PubMed:11166181, PubMed:22813744, PubMed:24319994). In this complex, acts as a GTPase-activating protein, by promoting GTP hydrolysis by eIF2G (EIF2S3) (PubMed:11166181). During scanning, interacts with both EIF1 (via its C-terminal domain (CTD)) and EIF1A (via its NTD) (PubMed:22813744). This interaction with EIF1A contributes to the maintenance of EIF1 within the open 43S PIC (PubMed:24319994). When start codon is recognized, EIF5, via its NTD, induces eIF2G (EIF2S3) to hydrolyze the GTP (PubMed:11166181). Start codon recognition also induces a conformational change of the PIC to a closed state (PubMed:22813744). This change increases the affinity of EIF5-CTD for EIF2-beta (EIF2S2), which allows the release, by an indirect mechanism, of EIF1 from the PIC (PubMed:22813744). Finally, EIF5 stabilizes the PIC in its closed conformation (PubMed:22813744).
Alternative names
Eukaryotic translation initiation factor 5, eIF-5, EIF5
Recommended products
Rabbit Polyclonal EIF5 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human Eukaryotic translation initiation factor 5 aa 100-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Eukaryotic translation initiation factor 5 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link P55010
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab85913 was affinity purified using an epitope specific to EIF5 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The eukaryotic translation initiation factor 5 (EIF5) plays an important role in the initiation phase of protein synthesis. EIF5 also known as eIF-5 is a protein that exhibits a molecular weight of approximately 49 kDa. It functions as a GTPase-activating protein that triggers the hydrolysis of GTP bound to the eukaryotic initiation factor 2 (eIF2) an essential step for the formation of the 80S ribosome complex during translation. EIF5 expression occurs widely across various tissues indicating its necessity for basic cellular functions.
- Biological function summary
EIF5 facilitates the translation initiation process which is critical for protein synthesis. It is an integral component of the multifactor eIF2 complex that ensures the accuracy of start codon recognition in mRNA. This complex assembly involves the association with other initiation factors like eIF1 and eIF3 orchestrating mRNA scanning and the selection of the start site for protein synthesis. EIF5's role in regulating protein biosynthesis links it to the maintenance of cellular homeostasis.
- Pathways
EIF5 serves a critical part in the translation initiation pathway. This pathway is essential for general protein biosynthesis directly impacting cellular growth and proliferation. It closely collaborates with related factors like eIF2 and eIF2B within this pathway to modulate translation in response to cellular stress and nutrient availability. Additionally EIF5 intersects the mTOR signaling pathway where it interacts with proteins that coordinate cell growth and metabolism under various physiological conditions.
- Associated diseases and disorders
EIF5 alterations can lead to disruptions in protein synthesis connecting it to certain human diseases like cancer. Some studies indicate a link between EIF5 dysregulation and tumorigenesis as faults in translation regulation can drive abnormal cell proliferation. Beyond cancer EIF5 may also intersect with neurodegenerative diseases given the translation regulation's importance in neuronal function. It can associate with other factors such as eIF2α wherein heightened EIF5 activity influences stress responses pivotal in disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-EIF5 antibody (ab85913)
Detection: Chemiluminescence
All lanes: Western blot - Anti-EIF5 antibody (ab85913) at 0.4 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Lane 5: NIH3T3 whole cell lysate at 50 µg
Predicted band size: 49 kDa
Observed band size: 49 kDa
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