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AB249587

Anti-EIF5 antibody [EPR12140(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal EIF5 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Eukaryotic translation initiation factor 5, eIF-5, EIF5

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-EIF5 antibody [EPR12140(B)] - BSA and Azide free (AB249587)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EIF5 antibody [EPR12140(B)] - BSA and Azide free (AB249587)

This data was developed using ab170915, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HepG2 cells labeling EIF5 with ab170915 at 1/100 dilution (green). DAPI nuclear staining (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF5 antibody [EPR12140(B)] - BSA and Azide free (AB249587)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF5 antibody [EPR12140(B)] - BSA and Azide free (AB249587)

This data was developed using ab170915, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling EIF5 with ab170915 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-EIF5 antibody [EPR12140(B)] - BSA and Azide free (AB249587)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-EIF5 antibody [EPR12140(B)] - BSA and Azide free (AB249587)

This data was developed using ab170915, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized K562 cells labeling EIF5 with ab170915 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

Western blot - Anti-EIF5 antibody [EPR12140(B)] - BSA and Azide free (AB249587)
  • WB

Supplier Data

Western blot - Anti-EIF5 antibody [EPR12140(B)] - BSA and Azide free (AB249587)

This data was developed using ab170915, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EIF5 antibody [EPR12140(B)] (<a href='/en-us/products/primary-antibodies/eif5-antibody-epr12140b-ab170915'>ab170915</a>) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP-conjugated at 1/2000 dilution

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12140(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249587 is the carrier-free version of ab170915.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic translation initiation factor 5 (EIF5) plays an important role in the initiation phase of protein synthesis. EIF5 also known as eIF-5 is a protein that exhibits a molecular weight of approximately 49 kDa. It functions as a GTPase-activating protein that triggers the hydrolysis of GTP bound to the eukaryotic initiation factor 2 (eIF2) an essential step for the formation of the 80S ribosome complex during translation. EIF5 expression occurs widely across various tissues indicating its necessity for basic cellular functions.
Biological function summary

EIF5 facilitates the translation initiation process which is critical for protein synthesis. It is an integral component of the multifactor eIF2 complex that ensures the accuracy of start codon recognition in mRNA. This complex assembly involves the association with other initiation factors like eIF1 and eIF3 orchestrating mRNA scanning and the selection of the start site for protein synthesis. EIF5's role in regulating protein biosynthesis links it to the maintenance of cellular homeostasis.

Pathways

EIF5 serves a critical part in the translation initiation pathway. This pathway is essential for general protein biosynthesis directly impacting cellular growth and proliferation. It closely collaborates with related factors like eIF2 and eIF2B within this pathway to modulate translation in response to cellular stress and nutrient availability. Additionally EIF5 intersects the mTOR signaling pathway where it interacts with proteins that coordinate cell growth and metabolism under various physiological conditions.

EIF5 alterations can lead to disruptions in protein synthesis connecting it to certain human diseases like cancer. Some studies indicate a link between EIF5 dysregulation and tumorigenesis as faults in translation regulation can drive abnormal cell proliferation. Beyond cancer EIF5 may also intersect with neurodegenerative diseases given the translation regulation's importance in neuronal function. It can associate with other factors such as eIF2α wherein heightened EIF5 activity influences stress responses pivotal in disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon (PubMed : 11166181, PubMed : 22813744, PubMed : 24319994). In this complex, acts as a GTPase-activating protein, by promoting GTP hydrolysis by eIF2G (EIF2S3) (PubMed : 11166181). During scanning, interacts with both EIF1 (via its C-terminal domain (CTD)) and EIF1A (via its NTD) (PubMed : 22813744). This interaction with EIF1A contributes to the maintenance of EIF1 within the open 43S PIC (PubMed : 24319994). When start codon is recognized, EIF5, via its NTD, induces eIF2G (EIF2S3) to hydrolyze the GTP (PubMed : 11166181). Start codon recognition also induces a conformational change of the PIC to a closed state (PubMed : 22813744). This change increases the affinity of EIF5-CTD for EIF2-beta (EIF2S2), which allows the release, by an indirect mechanism, of EIF1 from the PIC (PubMed : 22813744). Finally, EIF5 stabilizes the PIC in its closed conformation (PubMed : 22813744).
See full target information Eukaryotic translation initiation factor 5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 15:e5206 PubMed39959296

2025

Cell-Sonar, an Easy and Low-cost Method to Track a Target Protein by Expression Changes of Specific Protein Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Brockmöller,Lara Maria Molitor,Franz Worek,Simone Rothmiller
View all publications
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