AB137561

Anti-eIF5A antibody

Name: Anti-eIF5A antibody (ab137561) | Abcam
Brand: Abcam Limited
SKU: AB137561
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal eIF5A antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EIF5A.

View Alternative Names

Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, Rev-binding factor, eIF-4D, eIF-5A, EIF5A

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-eIF5A antibody (AB137561)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-eIF5A antibody (AB137561)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling EIF5A protein at cytoplasm and nucleus using ab137561 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). DAPI was used for nuclear staining (Blue).

Supplier Data

Western blot - Anti-eIF5A antibody (AB137561)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-eIF5A antibody (ab137561) at 1/1000 dilution

All lanes:

A431 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 17 kDa

false

Supplier Data

Western blot - Anti-eIF5A antibody (AB137561)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-eIF5A antibody (ab137561) at 1/1000 dilution

All lanes:

Mouse eye tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 17 kDa

false

Supplier Data

Western blot - Anti-eIF5A antibody (AB137561)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-eIF5A antibody (ab137561) at 1/5000 dilution

Lane 1:

PC-12 whole cell lysates at 30 µg

Lane 2:

Rat2 whole cell lysates at 30 µg

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human EIF5A.

P63241

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF5A also known as eukaryotic translation initiation factor 5A is a small protein with a molecular mass of approximately 18 kDa. It plays a significant mechanical role in the translation process by facilitating the formation of the first peptide bond in protein synthesis. eIF5A is expressed ubiquitously across various tissues and is noted for being the only protein known to undergo the unique post-translational modification called hypusination which is necessary for its function.

Biological function summary

EIF5A participates in regulating translation cell proliferation and apoptosis. eIF5A functions as part of a complex involved in ribosome-associated protein synthesis supporting the elongation of polyproline-containing peptides. It helps in maintaining cellular homeostasis by modulating the translation of specific sets of mRNAs that are critical for various cellular responses.

Pathways

EIF5A's role is particularly relevant in translation elongation and mRNA turnover pathways. It interacts closely with the elongation factors eEF2 and eEF1A to promote efficient protein synthesis. In the mRNA turnover pathway eIF5A has connections with proteins such as Dcp2 and Xrn1 which play a role in the degradation of mRNA thereby influencing mRNA stability and turnover.

EIF5A is linked to cancer and neurodegenerative diseases. Alterations in its function and expression levels have been observed in several cancer types highlighting its role in tumor progression. The protein's connection to apoptosis pathways suggests its involvement in neurodegenerative disorders such as Alzheimer's disease where cell death and tissue degeneration are prevalent. eIF5A associates with proteins like Bcl-2 in cancer and Tau in neurodegeneration indicating its varied impact on disease processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts (PubMed : 33547280). Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome : specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome (By similarity). Acts as a ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step (By similarity). Also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity (PubMed : 16987817). With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis (PubMed : 15371445). Regulates also TNF-alpha-mediated apoptosis (PubMed : 15452064, PubMed : 17187778). Mediates effects of polyamines on neuronal process extension and survival (PubMed : 17360499). Is required for autophagy by assisting the ribosome in translating the ATG3 protein at a specific amino acid sequence, the 'ASP-ASP-Gly' motif, leading to the increase of the efficiency of ATG3 translation and facilitation of LC3B lipidation and autophagosome formation (PubMed : 29712776).. (Microbial infection) Cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.

See full target information EIF5A

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 86:103814 PubMed40789258

2025

Inhibition of eIF5A hypusination enhances antioxidant defense to prevent kidney Ischemia/Reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Marc Cougnon,Sébastien Giraud,Maria Materozzi,Elisa Allart,Isabelle Rubera,Claire Mackowiak,Gisèle Jarretou,Nadège Boildieu,Virginie Ameteau,Estelle Lemarié,Hajar Ouahmi,Nicolas Melis,Mallorie Poet,Christophe Duranton,Luc Pellerin,Laurent Counillon,Marina Shkreli,Michel Tauc,Thierry Hauet,Didier F Pisani

Science advances 11:eadv2397 PubMed40531988

2025

Spermidine is a key polyamine required by intracellular parasites for survival within host erythrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Pallavi Singh,Jae-Yeon Choi,Emmanuel Cornillot,Choukri Ben Mamoun

Nature communications 15:2235 PubMed38472173

2024

Theileria parasites sequester host eIF5A to escape elimination by host-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Marie Villares,Nelly Lourenço,Ivan Ktorza,Jérémy Berthelet,Aristeidis Panagiotou,Aurélie Richard,Angélique Amo,Yulianna Koziy,Souhila Medjkane,Sergio Valente,Rossella Fioravanti,Catherine Pioche-Durieu,Laurent Lignière,Guillaume Chevreux,Antonello Mai,Jonathan B Weitzman

International journal of molecular sciences 22: PubMed33379337

2020

Yeast Translation Elongation Factor eIF5A Expression Is Regulated by Nutrient Availability through Different Signalling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Barba-Aliaga,Carlos Villarroel-Vicente,Alice Stanciu,Alba Corman,María Teresa Martínez-Pastor,Paula Alepuz

Scientific reports 9:1533 PubMed30733517

2019

eIF5A inhibition influences T cell dynamics in the pancreatic microenvironment of the humanized mouse model of Type 1 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Shahnawaz Imam,R Prathibha,Pervaiz Dar,Khalil Almotah,Ahmed Al-Khudhair,Syed Abdul-Moiz Hasan,Nancy Salim,Talha Naser Jilani,Raghavendra G Mirmira,Juan Carlos Jaume

The Journal of cell biology 207:213-23 PubMed25349259

2014

CLUH regulates mitochondrial biogenesis by binding mRNAs of nuclear-encoded mitochondrial proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Gao,Désirée Schatton,Paola Martinelli,Henriette Hansen,David Pla-Martin,Esther Barth,Christian Becker,Janine Altmueller,Peter Frommolt,Marco Sardiello,Elena I Rugarli

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-eIF5A antibody

Immunocytochemistry/ Immunofluorescence - Anti-eIF5A antibody (AB137561)

Western blot - Anti-eIF5A antibody (AB137561)

Western blot - Anti-eIF5A antibody (AB137561)

Western blot - Anti-eIF5A antibody (AB137561)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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