Rabbit Recombinant Monoclonal eIF5A2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Recombinant full length protein - Human, Mouse samples.
Images
![Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (AB248971), expandable thumbnail](https://content.abcam.com/products/images/eif5a2-eif5a-antibody-epr7411-105-bsa-and-azide-free-ab248971--western-blot-img101644.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (AB248971)")
![Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (AB248971), expandable thumbnail](https://content.abcam.com/products/images/eif5a2-eif5a-antibody-epr7411-105-bsa-and-azide-free-ab248971--western-blot-img28551.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (AB248971)")
![Flow Cytometry (Intracellular) - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (AB248971), expandable thumbnail](https://content.abcam.com/products/images/eif5a2-eif5a-antibody-epr7411-105-bsa-and-azide-free-ab248971--flow-cytometry-intracellular-img414689.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (AB248971)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC-P | ICC/IF | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Expected |
| Recombinant full length protein - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant full length protein - Human, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
1 product for Alternative Version
Target data
Function
Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts (PubMed:14622290). Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as a ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step (By similarity). Also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity (By similarity).
Additional Targets
EIF5A
Alternative names
Eukaryotic translation initiation factor 5A-2, eIF-5A-2, eIF-5A2, Eukaryotic initiation factor 5A isoform 2, EIF5A2
Recommended products
Rabbit Recombinant Monoclonal eIF5A2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Recombinant full length protein - Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7411-105
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248971 is the carrier-free version of Anti-eIF5A2 + eIF5A antibody [EPR7411-105] ab150403.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EIF5A2 also known as EIF5A isoform 2 and eIF5A or EIF5A isoform 1 play important mechanical roles in protein synthesis. eIF5A2 has a molecular mass of approximately 17 kDa while eIF5A shares a similar molecular weight. Both proteins are expressed throughout different tissues with higher levels observed in certain cancers and rapidly proliferating cells. eIF5A2 is particularly expressed in various tumor tissues and cell lines whereas eIF5A is expressed more ubiquitously.
- Biological function summary
These proteins facilitate the translation elongation process during protein synthesis. As part of the translation machinery they interact with ribosomes to promote the efficient synthesis of proteins. eIF5A and eIF5A2 are implicated in the hypusination pathway which activates them through a unique covalent modification. This modification is essential for their activity suggesting their critical involvement in cell growth and proliferation.
- Pathways
The hypusination pathway involves these proteins undergoing a post-translational modification that activates them. eIF5A and eIF5A2 are part of the eukaryotic translational elongation pathway which enhances their functional relevance in translational regulation. Their activity links closely with other translation-related proteins such as the eukaryotic initiation factor eIF2 which controls the overall rate of protein synthesis.
- Associated diseases and disorders
EIF5A2 and eIF5A have marked associations with cancer particularly colorectal and ovarian cancers. In these conditions overexpression and dysregulation of these proteins contribute to cancer progression and metastasis. Their connection with other proteins like the tumor suppressor p53 further highlights their role in tumor biology. Changes in eIF5A activity have also been linked to neurodegenerative diseases offering potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (ab248971), expandable thumbnail](https://content.abcam.com/products/images/eif5a2-eif5a-antibody-epr7411-105-bsa-and-azide-free-ab248971--western-blot-img101644.jpg "Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (ab248971)")
Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (ab248971)
This data was developed using Anti-eIF5A2 + eIF5A antibody [EPR7411-105] ab150403, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (ab248971) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: SW480 cell lysate at 10 µg
Lane 3: RAW264.7 cell lysate at 10 µg
Lane 4: NIH 3T3 cell lysate at 10 µg
Lane 5: 293T cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa
![Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (ab248971), expandable thumbnail](https://content.abcam.com/products/images/eif5a2-eif5a-antibody-epr7411-105-bsa-and-azide-free-ab248971--western-blot-img28551.jpg "Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (ab248971)")
Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (ab248971)
Blocking buffer: 5% NFDM/TBSTThis data was developed using Anti-eIF5A2 + eIF5A antibody [EPR7411-105] ab150403, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] (Anti-eIF5A2 + eIF5A antibody [EPR7411-105] ab150403) at 1/2000 dilution
Lane 1: Western blot - Recombinant Human eIF5A protein (Recombinant Human eIF5A protein ab87457) at 0.01 µg
Lane 2: Western blot - Recombinant Human eIF5A2 protein (Recombinant Human eIF5A2 protein ab99140) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa
Observed band size: 15 kDa, 20 kDa
Exposure time: 20s
![Flow Cytometry (Intracellular) - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (ab248971), expandable thumbnail](https://content.abcam.com/products/images/eif5a2-eif5a-antibody-epr7411-105-bsa-and-azide-free-ab248971--flow-cytometry-intracellular-img414689.jpg "Flow Cytometry (Intracellular) - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (ab248971)")
Flow Cytometry (Intracellular) - Anti-eIF5A2 + eIF5A antibody [EPR7411-105] - BSA and Azide free (ab248971)
This data was developed using Anti-eIF5A2 + eIF5A antibody [EPR7411-105] ab150403, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized HeLa cells labelling elF5A2 + eIF5A with Anti-eIF5A2 + eIF5A antibody [EPR7411-105] ab150403 at 1/10 dilution (red); compared to a negative rabbit IgG control (green).
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