Skip to main content

Rabbit Recombinant Monoclonal eIF5A2 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

Be the first to review this product! Submit a review

Images

Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (AB126735), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (AB126735), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (AB126735), expandable thumbnail
  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (AB126735), expandable thumbnail
  • OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (AB126735), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

3 products for Alternative Product

Target data

Function

Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts (PubMed:14622290). Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as a ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step (By similarity). Also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity (By similarity).

Additional Targets

EIF5A

Alternative names

Eukaryotic translation initiation factor 5A-2, eIF-5A-2, eIF-5A2, Eukaryotic initiation factor 5A isoform 2, EIF5A2

Recommended products

Rabbit Recombinant Monoclonal eIF5A2 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR7411-6
Purification technique
Affinity purification Protein A
Dissociation constant
5.5 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

This product was previously published with the name Anti-eIF5A2 antibody [EPR7411-6]. Following in-house testing that showed reactivity to both recombinant Human eIF5A protein (Recombinant Human eIF5A protein ab87457) and recombinant Human eIF5A2 protein (Recombinant Human eIF5A2 protein ab99140), the name was updated in January 2021 to Anti-eIF5A2 + eIF5A antibody [EPR7411-6].

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The eIF5A2 protein known also as eukaryotic translation initiation factor 5A2 mechanically functions as a translation elongation factor. It plays a significant role in protein synthesis by aiding in the elongation phase of translation. The eIF5A2 protein has a molecular weight of approximately 17 kDa. Expression of eIF5A2 occurs widely in various tissues with a notable presence in cancerous tissues indicating its potential role in tumorigenesis.

Biological function summary

The protein eIF5A2 is involved in diverse cellular processes beyond translation. It localizes in both the cytoplasm and nucleus affecting cell proliferation differentiation and apoptosis. eIF5A2 is not traditionally considered as part of a complex but interacts with ribosomes during the translation process to exert its function. It also influences the synthesis of specific proteins that are important for maintaining cell cycle machinery and responses to stress.

Pathways

EIF5A2 integrates into the regulation of translation and apoptosis pathways. One key pathway involves its links to the mTOR signaling pathway which affects cell growth and survival. Similar proteins involved in this network include eIF5A another isoform closely related to eIF5A2 and components of the ribosomal machinery. eIF5A2 through its effects on protein synthesis influences pathways that coordinate cellular responses to nutrient availability and growth signals.

Associated diseases and disorders

Cognitive disorders and various cancers involve the eIF5A2 protein. Aberrant expression and mutation of eIF5A2 can lead to unregulated protein synthesis contributing to cancer progression. The protein has connections with oncogenic pathways where alterations in its expression result in dysregulated cellular growth and survival. eIF5A2 interactions with proteins like c-Myc which is implicated in oncogenesis further establish its role in tumorigenic processes making it a target for cancer research and potential therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735), expandable thumbnail

    Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)

    All lanes: Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735) at 1/1000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: A431 cell lysate at 10 µg

    Lane 4: CaCo-2 cell lysate at 10 µg

    Lane 5: HepG2 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 17 kDa

  • Flow Cytometry (Intracellular) - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)

    Overlay histogram showing HeLa cells stained with ab126735 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126735, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)

    ab126735 at 1/100 dilution, labeling eIF5A2 + eIF5A in Formalin fixed Paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735), expandable thumbnail

    Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)

    Blocking buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735) at 1/2000 dilution

    Lane 1: Western blot - Recombinant Human eIF5A protein (Recombinant Human eIF5A protein ab87457) at 0.01 µg

    Lane 2: Western blot - Recombinant Human eIF5A2 protein (Recombinant Human eIF5A2 protein ab99140) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 17 kDa

    Observed band size: 15 kDa, 20 kDa

    Exposure time: 20s

  • OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735), expandable thumbnail

    OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7411-6] (ab126735)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com