AB126733

Anti-eIF5A2 + eIF5A antibody [EPR7412-47]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal eIF5A2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

Eukaryotic translation initiation factor 5A-2, eIF-5A-2, eIF-5A2, Eukaryotic initiation factor 5A isoform 2, EIF5A2

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (AB126733)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (AB126733)

ab126733, at 1/100 dilution, staining eIF5A2 + eIF5A in HepG2 cells by Immunofluorescence (green). DAPI counterstain (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (AB126733)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (AB126733)

ab126733 at 1/100 dilution, staining eIF5A2 + eIF5A in Formalin-fixed Paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (AB126733)

All lanes:

Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 17 kDa

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Lab

Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (AB126733)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human eIF5A protein (<a href='/en-us/products/proteins-peptides/recombinant-human-eif5a-protein-ab87457'>ab87457</a>) at 0.01 µg

Lane 2:

Western blot - Recombinant Human eIF5A2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-eif5a2-protein-ab99140'>ab99140</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 17 kDa

Observed band size: 15 kDa,20 kDa

true

Exposure time: 3min

OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (AB126733)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (AB126733)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7412-47

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% Ethylenediaminetetraacetic acid, disodium, dihydrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF5A2 also known as EIF5A isoform 2 and eIF5A or EIF5A isoform 1 play important mechanical roles in protein synthesis. eIF5A2 has a molecular mass of approximately 17 kDa while eIF5A shares a similar molecular weight. Both proteins are expressed throughout different tissues with higher levels observed in certain cancers and rapidly proliferating cells. eIF5A2 is particularly expressed in various tumor tissues and cell lines whereas eIF5A is expressed more ubiquitously.

Biological function summary

These proteins facilitate the translation elongation process during protein synthesis. As part of the translation machinery they interact with ribosomes to promote the efficient synthesis of proteins. eIF5A and eIF5A2 are implicated in the hypusination pathway which activates them through a unique covalent modification. This modification is essential for their activity suggesting their critical involvement in cell growth and proliferation.

Pathways

The hypusination pathway involves these proteins undergoing a post-translational modification that activates them. eIF5A and eIF5A2 are part of the eukaryotic translational elongation pathway which enhances their functional relevance in translational regulation. Their activity links closely with other translation-related proteins such as the eukaryotic initiation factor eIF2 which controls the overall rate of protein synthesis.

EIF5A2 and eIF5A have marked associations with cancer particularly colorectal and ovarian cancers. In these conditions overexpression and dysregulation of these proteins contribute to cancer progression and metastasis. Their connection with other proteins like the tumor suppressor p53 further highlights their role in tumor biology. Changes in eIF5A activity have also been linked to neurodegenerative diseases offering potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts (PubMed : 14622290). Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome : specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as a ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step (By similarity). Also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity (By similarity).

See full target information Eukaryotic translation initiation factor 5A-2

Additional targets

EIF5A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e70093 PubMed40271543

2025

Developmental deletion of amyloid precursor protein precludes transcriptional and proteomic responses to brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Lacovich,Maria Čarna,Sebastian J Novotný,Shanshan Wang,Kateřina Texlová,Kristina Locker Kovačovicova,Neda Dragišić,Daniel Havas,Brian P Head,Yonas E Geda,Clara Limbäck-Stokin,Gorazd Bernard Stokin

Journal of cancer research and clinical oncology 150:179 PubMed38584230

2024

Long non-coding RNA RAD51-AS1 promotes the tumorigenesis of ovarian cancer by elevating EIF5A2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhao,Jia Huang,Wenting Liu,Xiaoyan Su,Bei Zhao,Xianggang Wang,Xiaoju He

Cell death & disease 13:683 PubMed35931669

2022

Eukaryotic initiation factor 5A2 mediates hypoxia-induced autophagy and cisplatin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Guodong Xu,Hang Chen,Shibo Wu,Jiabin Chen,Shufen Zhang,Guofeng Shao,Lebo Sun,Yinyu Mu,Kaitai Liu,Qiaoling Pan,Ni Li,Xiaoxia An,Shuang Lin,Wei Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eIF5A2 + eIF5A antibody [EPR7412-47]

Immunocytochemistry/ Immunofluorescence - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (AB126733)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (AB126733)

Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (AB126733)

Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (AB126733)

OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (AB126733)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (3)

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