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Rabbit Recombinant Monoclonal eIF5A antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free (AB248991), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free (AB248991), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free (AB248991), expandable thumbnail
  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free (AB248991), expandable thumbnail
  • OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free (AB248991), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPIHC-PICC/IFWB
Human
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Predicted
Predicted
Expected
Rat
Not recommended
Predicted
Predicted
Expected

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts (PubMed:33547280). Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome (By similarity). Acts as a ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step (By similarity). Also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity (PubMed:16987817). With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis (PubMed:15371445). Regulates also TNF-alpha-mediated apoptosis (PubMed:15452064, PubMed:17187778). Mediates effects of polyamines on neuronal process extension and survival (PubMed:17360499). Is required for autophagy by assisting the ribosome in translating the ATG3 protein at a specific amino acid sequence, the 'ASP-ASP-Gly' motif, leading to the increase of the efficiency of ATG3 translation and facilitation of LC3B lipidation and autophagosome formation (PubMed:29712776). (Microbial infection) Cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.

Additional Targets

Eukaryotic translation initiation factor 5A-2

Alternative names

Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, Rev-binding factor, eIF-4D, eIF-5A, EIF5A

Recommended products

Rabbit Recombinant Monoclonal eIF5A antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7412-47
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248991 is the carrier-free version of Anti-eIF5A2 + eIF5A antibody [EPR7412-47] ab126733.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EIF5A2 also known as EIF5A isoform 2 and eIF5A or EIF5A isoform 1 play important mechanical roles in protein synthesis. eIF5A2 has a molecular mass of approximately 17 kDa while eIF5A shares a similar molecular weight. Both proteins are expressed throughout different tissues with higher levels observed in certain cancers and rapidly proliferating cells. eIF5A2 is particularly expressed in various tumor tissues and cell lines whereas eIF5A is expressed more ubiquitously.

Biological function summary

These proteins facilitate the translation elongation process during protein synthesis. As part of the translation machinery they interact with ribosomes to promote the efficient synthesis of proteins. eIF5A and eIF5A2 are implicated in the hypusination pathway which activates them through a unique covalent modification. This modification is essential for their activity suggesting their critical involvement in cell growth and proliferation.

Pathways

The hypusination pathway involves these proteins undergoing a post-translational modification that activates them. eIF5A and eIF5A2 are part of the eukaryotic translational elongation pathway which enhances their functional relevance in translational regulation. Their activity links closely with other translation-related proteins such as the eukaryotic initiation factor eIF2 which controls the overall rate of protein synthesis.

Associated diseases and disorders

EIF5A2 and eIF5A have marked associations with cancer particularly colorectal and ovarian cancers. In these conditions overexpression and dysregulation of these proteins contribute to cancer progression and metastasis. Their connection with other proteins like the tumor suppressor p53 further highlights their role in tumor biology. Changes in eIF5A activity have also been linked to neurodegenerative diseases offering potential targets for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free (ab248991), expandable thumbnail

    Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free (ab248991)

    This data was developed using Anti-eIF5A2 + eIF5A antibody [EPR7412-47] ab126733, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (Anti-eIF5A2 + eIF5A antibody [EPR7412-47] ab126733) at 1/1000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: Caco-2 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free (ab248991), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free (ab248991)

    This data was developed using Anti-eIF5A2 + eIF5A antibody [EPR7412-47] ab126733, the same antibody clone in a different buffer formulation.

    Anti-eIF5A2 + eIF5A antibody [EPR7412-47] ab126733 at 1/100 dilution, staining eIF5A2 + eIF5A in Formalin-fixed Paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free (ab248991), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free (ab248991)

    This data was developed using Anti-eIF5A2 + eIF5A antibody [EPR7412-47] ab126733, the same antibody clone in a different buffer formulation.

    Anti-eIF5A2 + eIF5A antibody [EPR7412-47] ab126733, at 1/100 dilution, staining eLF5A2 + eIF5A in HepG2 cells by Immunofluorescence (green). DAPI counterstain (blue).

  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free (ab248991), expandable thumbnail

    Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free (ab248991)

    Blocking buffer: 5% NFDM/TBST

    This data was developed using Anti-eIF5A2 + eIF5A antibody [EPR7412-47] ab126733, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (Anti-eIF5A2 + eIF5A antibody [EPR7412-47] ab126733) at 1/1000 dilution

    Lane 1: Western blot - Recombinant Human eIF5A protein (Recombinant Human eIF5A protein ab87457) at 0.01 µg

    Lane 2: Western blot - Recombinant Human eIF5A2 protein (Recombinant Human eIF5A2 protein ab99140) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 17 kDa

    Observed band size: 15 kDa, 20 kDa

    Exposure time: 3min

  • OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free (ab248991), expandable thumbnail

    OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] - BSA and Azide free (ab248991)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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