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Rabbit Recombinant Monoclonal eIF5A antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 12 publications.

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Images

Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (AB150439), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (AB150439), expandable thumbnail
  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (AB150439), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (AB150439), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (AB150439), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Human
Tested
Not recommended
Tested
Mouse
Predicted
Not recommended
Predicted
Rat
Predicted
Not recommended
Predicted

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

1 product for Alternative Version

Target data

Function

Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts (PubMed:33547280). Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome (By similarity). Acts as a ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step (By similarity). Also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity (PubMed:16987817). With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis (PubMed:15371445). Regulates also TNF-alpha-mediated apoptosis (PubMed:15452064, PubMed:17187778). Mediates effects of polyamines on neuronal process extension and survival (PubMed:17360499). Is required for autophagy by assisting the ribosome in translating the ATG3 protein at a specific amino acid sequence, the 'ASP-ASP-Gly' motif, leading to the increase of the efficiency of ATG3 translation and facilitation of LC3B lipidation and autophagosome formation (PubMed:29712776). (Microbial infection) Cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.

Additional Targets

Eukaryotic translation initiation factor 5A-2

Alternative names

Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, Rev-binding factor, eIF-4D, eIF-5A, EIF5A

Recommended products

Rabbit Recombinant Monoclonal eIF5A antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 12 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR7412-50
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EIF5A2 also known as EIF5A isoform 2 and eIF5A or EIF5A isoform 1 play important mechanical roles in protein synthesis. eIF5A2 has a molecular mass of approximately 17 kDa while eIF5A shares a similar molecular weight. Both proteins are expressed throughout different tissues with higher levels observed in certain cancers and rapidly proliferating cells. eIF5A2 is particularly expressed in various tumor tissues and cell lines whereas eIF5A is expressed more ubiquitously.

Biological function summary

These proteins facilitate the translation elongation process during protein synthesis. As part of the translation machinery they interact with ribosomes to promote the efficient synthesis of proteins. eIF5A and eIF5A2 are implicated in the hypusination pathway which activates them through a unique covalent modification. This modification is essential for their activity suggesting their critical involvement in cell growth and proliferation.

Pathways

The hypusination pathway involves these proteins undergoing a post-translational modification that activates them. eIF5A and eIF5A2 are part of the eukaryotic translational elongation pathway which enhances their functional relevance in translational regulation. Their activity links closely with other translation-related proteins such as the eukaryotic initiation factor eIF2 which controls the overall rate of protein synthesis.

Associated diseases and disorders

EIF5A2 and eIF5A have marked associations with cancer particularly colorectal and ovarian cancers. In these conditions overexpression and dysregulation of these proteins contribute to cancer progression and metastasis. Their connection with other proteins like the tumor suppressor p53 further highlights their role in tumor biology. Changes in eIF5A activity have also been linked to neurodegenerative diseases offering potential targets for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439), expandable thumbnail

    Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)

    All lanes: Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439) at 1/1000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: 293T cell lysate at 10 µg

    Lane 4: Raji cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP labelled antibody at 1/2000 dilution

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)

    Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labelling eIF5A2 + eIF5A with ab150439 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439), expandable thumbnail

    Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)

    Blocking buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439) at 1/1000 dilution

    Lane 1: Western blot - Recombinant Human eIF5A protein (Recombinant Human eIF5A protein ab87457) at 0.01 µg

    Lane 2: Western blot - Recombinant Human eIF5A2 protein (Recombinant Human eIF5A2 protein ab99140) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 17 kDa

    Observed band size: 15 kDa, 20 kDa

    Exposure time: 7s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling eIF5A2 + eIF5A with ab150439 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)

    Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab150439 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)

    Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150439 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-50] (ab150439)

    Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150439 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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