JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB251824

Anti-EIF5B antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal EIF5B antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EIF5B aa 1000-1150.

View Alternative Names

IF2, KIAA0741, EIF5B, Eukaryotic translation initiation factor 5B, eIF-5B, Translation initiation factor IF-2

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF5B antibody (AB251824)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF5B antibody (AB251824)

Paraffin-embedded human kidney tissue stained for EIF5B using ab251824 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EIF5B aa 1000-1150. The exact immunogen used to generate this antibody is proprietary information.

O60841

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF5B also known as eukaryotic translation initiation factor 5B plays a significant mechanical role in the initiation of mRNA translation. This protein has a molecular weight of approximately 140 kDa. EIF5B is highly conserved across eukaryotes and is found in the cytoplasm where its expression facilitates the assembly of the 80S ribosome. The protein interacts with the ribosomal subunits and GTP an energy-carrying molecule to promote the joining of the 40S and 60S subunits forming a functional unit ready for protein synthesis.
Biological function summary

EIF5B is important in facilitating vectorial movement during translation initiation. It plays a role in the ribosomal subunit assembly and interacts with several initiation factors and ribosomal proteins. EIF5B does not work alone; instead it is part of a complex that ensures the proper alignment and initiation of translation. By coordinating these interactions EIF5B ensures that protein synthesis starts at the correct part of the mRNA template.

Pathways

EIF5B is deeply involved in the translation initiation and protein synthesis pathways. The protein interacts with other initiation factors like eIF1A ensuring the fidelity of start codon recognition and subunit joining. EIF5B also associates with pathways related to ribosome biogenesis. These interactions position EIF5B as a link between the assembly of ribosomal machinery and the broader cellular pathways controlling protein synthesis.

EIF5B has connections to several pathologies particularly cancer and neurodegenerative diseases. Its role in ensuring accurate translation initiation makes aberrations in EIF5B linked to unregulated cell growth seen in cancers. In neurodegenerative disorders improper translation regulation can contribute to dysfunctional protein accumulation. Additional proteins such as eIF2B and eIF4E may also play interconnected roles in these diseases with a network of interactions influencing cellular outcomes in disease contexts.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays a role in translation initiation (PubMed : 10659855, PubMed : 35732735). Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon (PubMed : 10659855, PubMed : 35732735). Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex (PubMed : 12569173, PubMed : 35732735). Is released after formation of the 80S initiation complex (PubMed : 35732735). Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes (PubMed : 10659855, PubMed : 35732735). In contrast to its procaryotic homolog, does not promote recruitment of Met-rRNA to the small ribosomal subunit (PubMed : 10659855).
See full target information EIF5B
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 15:73 PubMed35718769

2022

Initiation and elongation factor co-expression correlates with recurrence and survival in epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Sobočan,Daniela Brunialti,Sussanne Sprung,Christoph Schatz,Jure Knez,Rajko Kavalar,Iztok Takač,Johannes Haybaeck

Nature communications 12:1172 PubMed33608523

2021

20S proteasomes secreted by the malaria parasite promote its growth.

Applications

Unspecified application

Species

Unspecified reactive species

Elya Dekel,Dana Yaffe,Irit Rosenhek-Goldian,Gili Ben-Nissan,Yifat Ofir-Birin,Mattia I Morandi,Tamar Ziv,Xavier Sisquella,Matthew A Pimentel,Thomas Nebl,Eugene Kapp,Yael Ohana Daniel,Paula Abou Karam,Daniel Alfandari,Ron Rotkopf,Shimrit Malihi,Tal Block Temin,Debakshi Mullick,Or-Yam Revach,Ariel Rudik,Nir S Gov,Ido Azuri,Ziv Porat,Giulia Bergamaschi,Raya Sorkin,Gijs J L Wuite,Ori Avinoam,Teresa G Carvalho,Sidney R Cohen,Michal Sharon,Neta Regev-Rudzki
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB22758

Anti-Nucleolin antibody

BOND RX™ Validated

Western blot - Anti-Nucleolin antibody (AB22758)

  • 4
  • 8
Primary Antibodies

AB169538

Anti-RPL8 antibody [EPR11630]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RPL8 antibody [EPR11630] (AB169538)

  • 0
  • 0
Primary Antibodies

AB86863

Anti-RPL5 antibody

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-RPL5 antibody (AB86863)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com