Rabbit Polyclonal EIF5B antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EIF5B aa 1000-1150.
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- Journal of ovarian research 15:732022Initiation and elongation factor co-expression correlates with recurrence and survival in epithelial ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonika Sobočan,Daniela Brunialti,Sussanne Sprung,Christoph Schatz,Jure Knez,Rajko Kavalar,Iztok Takač,Johannes HaybaeckPubMed 35718769
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human EIF5B aa 1000-1150. The exact immunogen used to generate this antibody is proprietary information. Database link O60841
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Orangutan | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Orangutan | Dilution info - | Notes - |
Target data
Function
Plays a role in translation initiation (PubMed:10659855, PubMed:35732735). Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon (PubMed:10659855, PubMed:35732735). Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex (PubMed:12569173, PubMed:35732735). Is released after formation of the 80S initiation complex (PubMed:35732735). Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes (PubMed:10659855, PubMed:35732735). In contrast to its procaryotic homolog, does not promote recruitment of Met-rRNA to the small ribosomal subunit (PubMed:10659855).
Alternative names
IF2, KIAA0741, EIF5B, Eukaryotic translation initiation factor 5B, eIF-5B, Translation initiation factor IF-2
Recommended products
Rabbit Polyclonal EIF5B antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EIF5B aa 1000-1150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human EIF5B aa 1000-1150. The exact immunogen used to generate this antibody is proprietary information. Database link O60841
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EIF5B also known as eukaryotic translation initiation factor 5B plays a significant mechanical role in the initiation of mRNA translation. This protein has a molecular weight of approximately 140 kDa. EIF5B is highly conserved across eukaryotes and is found in the cytoplasm where its expression facilitates the assembly of the 80S ribosome. The protein interacts with the ribosomal subunits and GTP an energy-carrying molecule to promote the joining of the 40S and 60S subunits forming a functional unit ready for protein synthesis.
- Biological function summary
EIF5B is important in facilitating vectorial movement during translation initiation. It plays a role in the ribosomal subunit assembly and interacts with several initiation factors and ribosomal proteins. EIF5B does not work alone; instead it is part of a complex that ensures the proper alignment and initiation of translation. By coordinating these interactions EIF5B ensures that protein synthesis starts at the correct part of the mRNA template.
- Pathways
EIF5B is deeply involved in the translation initiation and protein synthesis pathways. The protein interacts with other initiation factors like eIF1A ensuring the fidelity of start codon recognition and subunit joining. EIF5B also associates with pathways related to ribosome biogenesis. These interactions position EIF5B as a link between the assembly of ribosomal machinery and the broader cellular pathways controlling protein synthesis.
- Associated diseases and disorders
EIF5B has connections to several pathologies particularly cancer and neurodegenerative diseases. Its role in ensuring accurate translation initiation makes aberrations in EIF5B linked to unregulated cell growth seen in cancers. In neurodegenerative disorders improper translation regulation can contribute to dysfunctional protein accumulation. Additional proteins such as eIF2B and eIF4E may also play interconnected roles in these diseases with a network of interactions influencing cellular outcomes in disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF5B antibody (ab251824)
Paraffin-embedded human kidney tissue stained for EIF5B using ab251824 at 1/200 dilution in immunohistochemical analysis.
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