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AB89016

Anti-EIF5B antibody

4

(1 Review)

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(3 Publications)

Mouse Polyclonal EIF5B antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EIF5B.

View Alternative Names

IF2, KIAA0741, EIF5B, Eukaryotic translation initiation factor 5B, eIF-5B, Translation initiation factor IF-2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-EIF5B antibody (AB89016)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EIF5B antibody (AB89016)

ab89016 at 10μg/ml staining EIF5B on HeLa cell by immunofluorescence

Western blot - Anti-EIF5B antibody (AB89016)
  • WB

Unknown

Western blot - Anti-EIF5B antibody (AB89016)

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Western blot - Anti-EIF5B antibody (ab89016) at 5 µg/mL

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Jurkat cell lysate at 50 µg

Predicted band size: 139 kDa

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Western blot - Anti-EIF5B antibody (AB89016)
  • WB

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Western blot - Anti-EIF5B antibody (AB89016)

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Western blot - Anti-EIF5B antibody (ab89016) at 5 µg/mL

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Human placenta tissue lysate at 50 µg

Predicted band size: 139 kDa

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Western blot - Anti-EIF5B antibody (AB89016)
  • WB

Unknown

Western blot - Anti-EIF5B antibody (AB89016)

All lanes:

Western blot - Anti-EIF5B antibody (ab89016) at 5 µg/mL

Lane 1:

EIF5B transfected 293T cell lysate at 50 µg

Lane 2:

Non-transfected lysate at 50 µg

Predicted band size: 139 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human EIF5B.

O60841

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF5B also known as eukaryotic translation initiation factor 5B plays a significant mechanical role in the initiation of mRNA translation. This protein has a molecular weight of approximately 140 kDa. EIF5B is highly conserved across eukaryotes and is found in the cytoplasm where its expression facilitates the assembly of the 80S ribosome. The protein interacts with the ribosomal subunits and GTP an energy-carrying molecule to promote the joining of the 40S and 60S subunits forming a functional unit ready for protein synthesis.
Biological function summary

EIF5B is important in facilitating vectorial movement during translation initiation. It plays a role in the ribosomal subunit assembly and interacts with several initiation factors and ribosomal proteins. EIF5B does not work alone; instead it is part of a complex that ensures the proper alignment and initiation of translation. By coordinating these interactions EIF5B ensures that protein synthesis starts at the correct part of the mRNA template.

Pathways

EIF5B is deeply involved in the translation initiation and protein synthesis pathways. The protein interacts with other initiation factors like eIF1A ensuring the fidelity of start codon recognition and subunit joining. EIF5B also associates with pathways related to ribosome biogenesis. These interactions position EIF5B as a link between the assembly of ribosomal machinery and the broader cellular pathways controlling protein synthesis.

EIF5B has connections to several pathologies particularly cancer and neurodegenerative diseases. Its role in ensuring accurate translation initiation makes aberrations in EIF5B linked to unregulated cell growth seen in cancers. In neurodegenerative disorders improper translation regulation can contribute to dysfunctional protein accumulation. Additional proteins such as eIF2B and eIF4E may also play interconnected roles in these diseases with a network of interactions influencing cellular outcomes in disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in translation initiation (PubMed : 10659855, PubMed : 35732735). Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon (PubMed : 10659855, PubMed : 35732735). Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex (PubMed : 12569173, PubMed : 35732735). Is released after formation of the 80S initiation complex (PubMed : 35732735). Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes (PubMed : 10659855, PubMed : 35732735). In contrast to its procaryotic homolog, does not promote recruitment of Met-rRNA to the small ribosomal subunit (PubMed : 10659855).
See full target information EIF5B
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 30:77-87 PubMed37305324

2023

IGF2BP3-induced activation of EIF5B contributes to progression of hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyin Li,Qian Wang,Hongfeng Liang,Shisheng Chen,Haiwen Chen,Yaoyong Lu,Changfu Yang

Science advances 7:eabi5751 PubMed34890234

2021

The nuclear transcription factor, TAF7, is a cytoplasmic regulator of protein synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Cheng,Kevin Semmens,Elizabeth McManus,Qingrong Chen,Daoud Meerzaman,Xiantao Wang,Markus Hafner,Brian A Lewis,Hidehisa Takahashi,Ballachanda N Devaiah,Anne Gegonne,Dinah S Singer

Lipids 53:561-580 PubMed30203570

2018

Impact of Fabp1 Gene Ablation on Uptake and Degradation of Endocannabinoids in Mouse Hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Avery L McIntosh,Huan Huang,Danilo Landrock,Gregory G Martin,Shengrong Li,Ann B Kier,Friedhelm Schroeder
View all publications
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