AB308374

Anti-EIF6 antibody [HL1758]

Recombinant
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Rabbit Recombinant Monoclonal eIF-6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat, Zebrafish, Drosophila melanogaster samples. Immunogen corresponding to Recombinant Fragment Protein within Human EIF6.

View Alternative Names

EIF3A, ITGB4BP, OK/SW-cl.27, EIF6, Eukaryotic translation initiation factor 6, eIF-6, B(2)GCN homolog, B4 integrin interactor, CAB, p27(BBP)

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF6 antibody [HL1758] (AB308374)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF6 antibody [HL1758] (AB308374)

Paraffin-embedded human esophageal carcinoma tissue stained for EIF6 protein at cytoplasm and nucleus with ab308374 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-EIF6 antibody [HL1758] (AB308374)

All lanes:

Western blot - Anti-EIF6 antibody [HL1758] (ab308374) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extract at 30 µg

Lane 2:

Transfected 293T whole cell extract with EIF6 shRNA 1 at 30 µg

Lane 3:

Transfected 293T whole cell extract with EIF6 shRNA 2 at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 27 kDa

false

Supplier Data

Western blot - Anti-EIF6 antibody [HL1758] (AB308374)

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [HL1758] (ab308374) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-EIF6 antibody [HL1758] (ab308374) at 1/1000 dilution

Lane 1:

A549 cell lysate at 30 µg

Lane 2:

H1299 cell lysate at 30 µg

Lane 3:

HCT116 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 26.6 kDa

false

Supplier Data

Western blot - Anti-EIF6 antibody [HL1758] (AB308374)

Various whole cell extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [HL1758] (ab308374) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-EIF6 antibody [HL1758] (ab308374) at 1/1000 dilution

Lane 1:

Neuro2A cell lysate at 50 µg

Lane 2:

Rat2 cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 26.6 kDa

false

Supplier Data

Western blot - Anti-EIF6 antibody [HL1758] (AB308374)

All lanes:

Western blot - Anti-EIF6 antibody [HL1758] (ab308374) at 1/1000 dilution

All lanes:

Whole S2 cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 27 kDa

false

Supplier Data

Western blot - Anti-EIF6 antibody [HL1758] (AB308374)

Separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-EIF6 antibody [HL1758] (ab308374) at 1/1000 dilution

All lanes:

Whole zebrafish lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 26.6 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1758

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat, Zebrafish, Drosophila melanogaster

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human EIF6. The exact immunogen used to generate this antibody is proprietary information.

P56537

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Drosophila melanogaster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF-6 also known as eukaryotic translation initiation factor 6 is a protein involved in the control of ribosomal function. The protein has a molecular mass of approximately 25 kDa and is expressed in the cytoplasm of eukaryotic cells. It acts by binding to the 60S ribosomal subunit and preventing premature association with the 40S subunit. This regulation ensures the proper assembly and function of the 80S ribosome essential for protein synthesis.

Biological function summary

Eukaryotic translation initiation factor 6 plays a significant role in ribosome biogenesis and translation initiation. It functions as a part of the ribosomal precursor complex facilitating the formation of functional 60S ribosomal units. Through its action eIF-6 modulates cellular protein synthesis rates impacting cell growth and proliferation. Additionally eIF-6 influences cellular responses to nutrient availability stressing its role in metabolic regulation.

Pathways

Eukaryotic translation initiation factor 6 is integral to the mTOR signaling pathway and ribosome biogenesis pathway. The mTOR pathway involves proteins like mTOR itself and S6 kinase which connect to eIF-6-mediated regulation of protein synthesis. eIF-6 maintains the balance between ribosome production and protein translation rates coordinating cellular growth with environmental cues.

Aberrations in eukaryotic translation initiation factor 6 levels link to cancer and metabolic diseases. Overexpression of eIF-6 can lead to excessive protein synthesis contributing to oncogenic processes by promoting unchecked cell proliferation. Additionally the dysregulation of eIF-6 can affect the mTOR pathway contributing to metabolic dysfunction. Connections between eIF-6 and other proteins like cyclin D1 highlight its impact on tumorigenesis and cell cycle control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm (PubMed : 10085284, PubMed : 14654845, PubMed : 21536732, PubMed : 32669547). Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity (PubMed : 10085284, PubMed : 14654845, PubMed : 21536732). In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis (By similarity). Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (PubMed : 17507929). Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth (By similarity).

See full target information EIF6

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EIF6 antibody [HL1758]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF6 antibody [HL1758] (AB308374)

Western blot - Anti-EIF6 antibody [HL1758] (AB308374)

Western blot - Anti-EIF6 antibody [HL1758] (AB308374)

Western blot - Anti-EIF6 antibody [HL1758] (AB308374)

Western blot - Anti-EIF6 antibody [HL1758] (AB308374)

Western blot - Anti-EIF6 antibody [HL1758] (AB308374)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise