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Rabbit Recombinant Monoclonal eIF-6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat, Zebrafish, Drosophila melanogaster samples. Immunogen corresponding to Recombinant Fragment Protein within Human EIF6.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF6 antibody [HL1758] (AB308374), expandable thumbnail
  • Western blot - Anti-EIF6 antibody [HL1758] (AB308374), expandable thumbnail
  • Western blot - Anti-EIF6 antibody [HL1758] (AB308374), expandable thumbnail
  • Western blot - Anti-EIF6 antibody [HL1758] (AB308374), expandable thumbnail
  • Western blot - Anti-EIF6 antibody [HL1758] (AB308374), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human EIF6. The exact immunogen used to generate this antibody is proprietary information. Database link P56537

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected
Drosophila melanogaster
Tested
Expected
Zebrafish
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/500 - 1/3000
Notes

-

Species
Mouse
Dilution info
1/500 - 1/3000
Notes

-

Species
Rat
Dilution info
1/500 - 1/3000
Notes

-

Species
Zebrafish
Dilution info
1/500 - 1/3000
Notes

-

Species
Drosophila melanogaster
Dilution info
1/500 - 1/3000
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Zebrafish, Drosophila melanogaster
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm (PubMed:10085284, PubMed:14654845, PubMed:21536732, PubMed:32669547). Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity (PubMed:10085284, PubMed:14654845, PubMed:21536732). In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis (By similarity). Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (PubMed:17507929). Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth (By similarity).

Alternative names

EIF3A, ITGB4BP, OK/SW-cl.27, EIF6, Eukaryotic translation initiation factor 6, eIF-6, B(2)GCN homolog, B4 integrin interactor, CAB, p27(BBP)

Recommended products

Rabbit Recombinant Monoclonal eIF-6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat, Zebrafish, Drosophila melanogaster samples. Immunogen corresponding to Recombinant Fragment Protein within Human EIF6.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human EIF6. The exact immunogen used to generate this antibody is proprietary information. Database link P56537
Carrier free
Yes
Clone number
HL1758
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EIF-6 also known as eukaryotic translation initiation factor 6 is a protein involved in the control of ribosomal function. The protein has a molecular mass of approximately 25 kDa and is expressed in the cytoplasm of eukaryotic cells. It acts by binding to the 60S ribosomal subunit and preventing premature association with the 40S subunit. This regulation ensures the proper assembly and function of the 80S ribosome essential for protein synthesis.

Biological function summary

Eukaryotic translation initiation factor 6 plays a significant role in ribosome biogenesis and translation initiation. It functions as a part of the ribosomal precursor complex facilitating the formation of functional 60S ribosomal units. Through its action eIF-6 modulates cellular protein synthesis rates impacting cell growth and proliferation. Additionally eIF-6 influences cellular responses to nutrient availability stressing its role in metabolic regulation.

Pathways

Eukaryotic translation initiation factor 6 is integral to the mTOR signaling pathway and ribosome biogenesis pathway. The mTOR pathway involves proteins like mTOR itself and S6 kinase which connect to eIF-6-mediated regulation of protein synthesis. eIF-6 maintains the balance between ribosome production and protein translation rates coordinating cellular growth with environmental cues.

Associated diseases and disorders

Aberrations in eukaryotic translation initiation factor 6 levels link to cancer and metabolic diseases. Overexpression of eIF-6 can lead to excessive protein synthesis contributing to oncogenic processes by promoting unchecked cell proliferation. Additionally the dysregulation of eIF-6 can affect the mTOR pathway contributing to metabolic dysfunction. Connections between eIF-6 and other proteins like cyclin D1 highlight its impact on tumorigenesis and cell cycle control.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF6 antibody [HL1758] (ab308374), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF6 antibody [HL1758] (ab308374)

    Paraffin-embedded human esophageal carcinoma tissue stained for EIF6 protein at cytoplasm and nucleus with ab308374 at a 1/100 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Western blot - Anti-EIF6 antibody [HL1758] (ab308374), expandable thumbnail

    Western blot - Anti-EIF6 antibody [HL1758] (ab308374)

    All lanes: Western blot - Anti-EIF6 antibody [HL1758] (ab308374) at 1/5000 dilution

    Lane 1: Non-transfected 293T whole cell extract at 30 µg

    Lane 2: Transfected 293T whole cell extract with EIF6 shRNA 1 at 30 µg

    Lane 3: Transfected 293T whole cell extract with EIF6 shRNA 2 at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 27 kDa

  • Western blot - Anti-EIF6 antibody [HL1758] (ab308374), expandable thumbnail

    Western blot - Anti-EIF6 antibody [HL1758] (ab308374)

    All lanes: Western blot - Anti-EIF6 antibody [HL1758] (ab308374) at 1/1000 dilution

    All lanes: Whole S2 cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 27 kDa

  • Western blot - Anti-EIF6 antibody [HL1758] (ab308374), expandable thumbnail

    Western blot - Anti-EIF6 antibody [HL1758] (ab308374)

    Separated by 12% SDS-PAGE.

    All lanes: Western blot - Anti-EIF6 antibody [HL1758] (ab308374) at 1/1000 dilution

    All lanes: Whole zebrafish lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 26.6 kDa

  • Western blot - Anti-EIF6 antibody [HL1758] (ab308374), expandable thumbnail

    Western blot - Anti-EIF6 antibody [HL1758] (ab308374)

    Various whole cell extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [HL1758] (ab308374) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-EIF6 antibody [HL1758] (ab308374) at 1/1000 dilution

    Lane 1: Neuro2A cell lysate at 50 µg

    Lane 2: Rat2 cell lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 26.6 kDa

  • Western blot - Anti-EIF6 antibody [HL1758] (ab308374), expandable thumbnail

    Western blot - Anti-EIF6 antibody [HL1758] (ab308374)

    Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [HL1758] (ab308374) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-EIF6 antibody [HL1758] (ab308374) at 1/1000 dilution

    Lane 1: A549 cell lysate at 30 µg

    Lane 2: H1299 cell lysate at 30 µg

    Lane 3: HCT116 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 26.6 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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