AB205948

Anti-ELAC2 antibody

Name: Anti-ELAC2 antibody (ab205948) | Abcam
Brand: Abcam Limited
SKU: AB205948

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal ELAC2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ELAC2 aa 150 to C-terminus.

View Alternative Names

HPC2, ELAC2, Zinc phosphodiesterase ELAC protein 2, ElaC homolog protein 2, Heredity prostate cancer protein 2, Ribonuclease Z 2, tRNA 3 endonuclease 2, tRNase Z 2, RNase Z 2

2 Images

Supplier Data

Immunoprecipitation - Anti-ELAC2 antibody (AB205948)

Detection of ELAC2 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205948 at 6 μg/mg lysate for IP and 0.4μg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-ELAC2 antibody (ab205948)

Predicted band size: 92 kDa

false

Supplier Data

Western blot - Anti-ELAC2 antibody (AB205948)

All lanes:

Western blot - Anti-ELAC2 antibody (ab205948) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 92 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ELAC2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BQ52

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELAC2 also known as ElaC ribonuclease Z 2 functions as an RNase Z-like enzyme with primary responsibility for processing tRNA molecules by cutting the 3' ends. With a mass of approximately 93 kDa ELAC2 localizes mainly in the nucleus and mitochondria expressing in various human tissues. As an essential component of RNA maturation mechanisms it ensures appropriate RNA synthesis and function within cellular contexts.

Biological function summary

The ELAC2 protein contributes significantly to the maturation and stability of transfer RNAs. It forms a part of the ribonuclease Z (RNase Z) family. ELAC2 activity facilitates the specific removal of 3′ trailer sequences from precursor tRNAs which is important for tRNA functionality and stability. It partners with other proteins to maintain cellular homeostasis and ensure accurate protein translation by processing tRNA precursors effectively.

Pathways

ELAC2 integrates into the broader RNA processing and stability pathways highlighting its significant role in tRNA maturation. Within these pathways ELAC2 interacts with proteins like ELAC1 which share similar catalytic activities albeit with different specificity or localization. These interactions and active participation in RNA processing pathways demonstrate ELAC2’s importance toward maintaining proper cellular function and gene expression regulation.

ELAC2 has associations with prostate cancer and cardiac hypertrophy. In prostate cancer ELAC2 mutations or dysregulation may result in altered RNA processing affecting cellular growth and differentiation. Moreover in cardiac hypertrophy ELAC2 potentially influences myocardial growth by impacting mitochondrial RNA processing. ELAC2-related dysfunctions could therefore offer insight into disease mechanisms alongside proteins like RNASEL which also contribute to these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Zinc phosphodiesterase, which displays mitochondrial tRNA 3'-processing endonuclease activity. Involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA (PubMed : 21593607). Associates with mitochondrial DNA complexes at the nucleoids to initiate RNA processing and ribosome assembly (PubMed : 24703694).

See full target information ELAC2

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 17:e1009285 PubMed33513160

2021

Genome-scale CRISPR screening for modifiers of cellular LDL uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Brian T Emmer,Emily J Sherman,Paul J Lascuna,Sarah E Graham,Cristen J Willer,David Ginsburg

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ELAC2 antibody

Immunoprecipitation - Anti-ELAC2 antibody (AB205948)

Western blot - Anti-ELAC2 antibody (AB205948)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise