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AB251725

Anti-ELAC2 antibody

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(1 Publication)

Rabbit Polyclonal ELAC2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ELAC2 aa 350-500.

View Alternative Names

HPC2, ELAC2, Zinc phosphodiesterase ELAC protein 2, ElaC homolog protein 2, Heredity prostate cancer protein 2, Ribonuclease Z 2, tRNA 3 endonuclease 2, tRNase Z 2, RNase Z 2

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-ELAC2 antibody (AB251725)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ELAC2 antibody (AB251725)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for ELAC2 (green) using ab251725 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAC2 antibody (AB251725)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAC2 antibody (AB251725)

Paraffin-embedded human skin tissue stained for ELAC2 using ab251725 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAC2 antibody (AB251725)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAC2 antibody (AB251725)

Paraffin-embedded human testis tissue stained for ELAC2 using ab251725 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAC2 antibody (AB251725)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAC2 antibody (AB251725)

Paraffin-embedded human fallopian tube tissue stained for ELAC2 using ab251725 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAC2 antibody (AB251725)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAC2 antibody (AB251725)

Paraffin-embedded human lymph node tissue stained for ELAC2 using ab251725 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-ELAC2 antibody (AB251725)
  • WB

Unknown

Western blot - Anti-ELAC2 antibody (AB251725)

All lanes:

Western blot - Anti-ELAC2 antibody (ab251725) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) whole cell lysate

Predicted band size: 92 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ELAC2 aa 350-500. The exact immunogen used to generate this antibody is proprietary information.

Q9BQ52

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELAC2 also known as ElaC ribonuclease Z 2 functions as an RNase Z-like enzyme with primary responsibility for processing tRNA molecules by cutting the 3' ends. With a mass of approximately 93 kDa ELAC2 localizes mainly in the nucleus and mitochondria expressing in various human tissues. As an essential component of RNA maturation mechanisms it ensures appropriate RNA synthesis and function within cellular contexts.
Biological function summary

The ELAC2 protein contributes significantly to the maturation and stability of transfer RNAs. It forms a part of the ribonuclease Z (RNase Z) family. ELAC2 activity facilitates the specific removal of 3′ trailer sequences from precursor tRNAs which is important for tRNA functionality and stability. It partners with other proteins to maintain cellular homeostasis and ensure accurate protein translation by processing tRNA precursors effectively.

Pathways

ELAC2 integrates into the broader RNA processing and stability pathways highlighting its significant role in tRNA maturation. Within these pathways ELAC2 interacts with proteins like ELAC1 which share similar catalytic activities albeit with different specificity or localization. These interactions and active participation in RNA processing pathways demonstrate ELAC2’s importance toward maintaining proper cellular function and gene expression regulation.

ELAC2 has associations with prostate cancer and cardiac hypertrophy. In prostate cancer ELAC2 mutations or dysregulation may result in altered RNA processing affecting cellular growth and differentiation. Moreover in cardiac hypertrophy ELAC2 potentially influences myocardial growth by impacting mitochondrial RNA processing. ELAC2-related dysfunctions could therefore offer insight into disease mechanisms alongside proteins like RNASEL which also contribute to these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Zinc phosphodiesterase, which displays mitochondrial tRNA 3'-processing endonuclease activity. Involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA (PubMed : 21593607). Associates with mitochondrial DNA complexes at the nucleoids to initiate RNA processing and ribosome assembly (PubMed : 24703694).
See full target information ELAC2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of diabetes and clinical research 5:1-10 PubMed37304310

2023

Elevated Opioid Growth Factor Alters the Limbus in Type 1 Diabetic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia J McLaughlin,Joseph W Sassani,David Diaz,Ian S Zagon
View all publications
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