AB46774

Anti-Elafin/ESI antibody

Name: Anti-Elafin/ESI antibody (ab46774) | Abcam
Brand: Abcam Limited
SKU: AB46774
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Elafin/ESI antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PI3.

View Alternative Names

WAP3, WFDC14, PI3, Elafin, Elastase-specific inhibitor, Peptidase inhibitor 3, Protease inhibitor WAP3, Skin-derived antileukoproteinase, WAP four-disulfide core domain protein 14, ESI, PI-3, SKALP

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Elafin/ESI antibody (AB46774)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Elafin/ESI antibody (AB46774)

ICC/IF image of ab46774 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46774, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-Elafin/ESI antibody (AB46774)

All lanes:

Western blot - Anti-Elafin/ESI antibody (ab46774) at 1/1000 dilution

Lane 1:

recombinant Human Elafin at 0.05 µg

Lane 2:

recombinant Human Elafin at 0.01 µg

Lane 3:

recombinant Human Elafin at 0.001 µg

Predicted band size: 12 kDa

Observed band size: 14 kDa,18 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human PI3. The exact immunogen used to generate this antibody is proprietary information.

P19957

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 2.9% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Elafin also known as ESI or ESI protein is a small serine protease inhibitor with a molecular mass of around 6 kDa. This protein produced by epithelial cells resides predominantly in the skin lungs and other mucosal surfaces. The Elafin protein plays an important mechanical role in inhibiting neutrophil elastase and proteinase 3. It contributes to the regulation of protease activity within tissues controlling inflammation and repair processes.

Biological function summary

Elafin interacts intimately with protease inhibitors to guard tissues against excessive proteolytic damage. It does not typically form large complexes but functions as part of the innate immune defense. This protein helps to neutralize the action of neutrophil elastase which can help maintain the integrity of connective tissue extracellular matrix. Despite its standalone function Elafin's activity serves in tandem with other inhibitors in the protease-antiprotease balance.

Pathways

Elafin integrates prominently into the proteolytic and inflammatory pathways. It functions particularly in the inflammation and immune response pathways coordinating with proteins like secretory leukocyte protease inhibitor (SLPI) and alpha-1 antitrypsin. Elafin inhibits enzymes that degrade connective tissue components. Therefore it plays an important role in modulating inflammatory responses and tissue remodeling post-injury.

Connections between Elafin and conditions such as chronic obstructive pulmonary disease (COPD) and psoriasis appear significant. These diseases relate to abnormal protease activity and chronic inflammation where Elafin's inhibitory function can be affected or diminished. Studies have shown that altered Elafin levels link with these diseases alongside proteins like neutrophil elastase and matrix metalloproteinases which contribute to tissue damage. Researchers continue to explore Elafin's therapeutic potential in managing such inflammatory conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Has been shown to inhibit the alpha-4-beta-2/CHRNA2-CHRNB2 nicotinic acetylcholine receptor and to produce a weak inhibition on Kv11.1/KCNH2/ERG1 and on the transient receptor potential cation channel subfamily V member 1 (TRPV1) (PubMed : 29483648).

See full target information PI3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 17:4 PubMed30642362

2019

Overexpression of MTA1 inhibits the metastatic ability of ZR-75-30 cells in vitro by promoting MTA2 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Long Zhang,Qi Wang,Yuzhen Zhou,Qianwen Ouyang,Weixing Dai,Jianfeng Chen,Peipei Ding,Ling Li,Xin Zhang,Wei Zhang,Xinyue Lv,Luying Li,Pingzhao Zhang,Guoxiang Cai,Weiguo Hu

Stem cell research & therapy 8:157 PubMed28676123

2017

Antimicrobial peptides secreted by equine mesenchymal stromal cells inhibit the growth of bacteria commonly found in skin wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca M Harman,Steven Yang,Megan K He,Gerlinde R Van de Walle

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Elafin/ESI antibody

Immunocytochemistry/ Immunofluorescence - Anti-Elafin/ESI antibody (AB46774)

Western blot - Anti-Elafin/ESI antibody (AB46774)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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