Rabbit Polyclonal Elafin/ESI antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PI3.
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- Cell communication and signaling : CCS 17:42019Overexpression of MTA1 inhibits the metastatic ability of ZR-75-30 cells in vitro by promoting MTA2 degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLong Zhang et. al.PubMed 30642362
- Stem cell research & therapy 8:1572017Antimicrobial peptides secreted by equine mesenchymal stromal cells inhibit the growth of bacteria commonly found in skin wounds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRebecca M Harman et. al.PubMed 28676123
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 2.9% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PI3. The exact immunogen used to generate this antibody is proprietary information. Database link P19957
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Associated Products
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Target data
Function
Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Has been shown to inhibit the alpha-4-beta-2/CHRNA2-CHRNB2 nicotinic acetylcholine receptor and to produce a weak inhibition on Kv11.1/KCNH2/ERG1 and on the transient receptor potential cation channel subfamily V member 1 (TRPV1) (PubMed:29483648).
Alternative names
WAP3, WFDC14, PI3, Elafin, Elastase-specific inhibitor, Peptidase inhibitor 3, Protease inhibitor WAP3, Skin-derived antileukoproteinase, WAP four-disulfide core domain protein 14, ESI, PI-3, SKALP
Recommended products
Rabbit Polyclonal Elafin/ESI antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PI3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 2.9% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PI3. The exact immunogen used to generate this antibody is proprietary information. Database link P19957
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Elafin also known as ESI or ESI protein is a small serine protease inhibitor with a molecular mass of around 6 kDa. This protein produced by epithelial cells resides predominantly in the skin lungs and other mucosal surfaces. The Elafin protein plays an important mechanical role in inhibiting neutrophil elastase and proteinase 3. It contributes to the regulation of protease activity within tissues controlling inflammation and repair processes.
- Biological function summary
Elafin interacts intimately with protease inhibitors to guard tissues against excessive proteolytic damage. It does not typically form large complexes but functions as part of the innate immune defense. This protein helps to neutralize the action of neutrophil elastase which can help maintain the integrity of connective tissue extracellular matrix. Despite its standalone function Elafin's activity serves in tandem with other inhibitors in the protease-antiprotease balance.
- Pathways
Elafin integrates prominently into the proteolytic and inflammatory pathways. It functions particularly in the inflammation and immune response pathways coordinating with proteins like secretory leukocyte protease inhibitor (SLPI) and alpha-1 antitrypsin. Elafin inhibits enzymes that degrade connective tissue components. Therefore it plays an important role in modulating inflammatory responses and tissue remodeling post-injury.
- Associated diseases and disorders
Connections between Elafin and conditions such as chronic obstructive pulmonary disease (COPD) and psoriasis appear significant. These diseases relate to abnormal protease activity and chronic inflammation where Elafin's inhibitory function can be affected or diminished. Studies have shown that altered Elafin levels link with these diseases alongside proteins like neutrophil elastase and matrix metalloproteinases which contribute to tissue damage. Researchers continue to explore Elafin's therapeutic potential in managing such inflammatory conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Elafin/ESI antibody (ab46774)
All lanes: Western blot - Anti-Elafin/ESI antibody (ab46774) at 1/1000 dilution
Lane 1: recombinant Human Elafin at 0.05 µg
Lane 2: recombinant Human Elafin at 0.01 µg
Lane 3: recombinant Human Elafin at 0.001 µg
Predicted band size: 12 kDa
Observed band size: 14 kDa, 18 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Elafin/ESI antibody (ab46774)
ICC/IF image of ab46774 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46774, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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