AB231117

Anti-Elastase-1 antibody

Name: Anti-Elastase-1 antibody (ab231117) | Abcam
Brand: Abcam Limited
SKU: AB231117

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(2 Publications)

Rabbit Polyclonal Elastase-1 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Cela1 aa 1 to C-terminus.

View Alternative Names

Ela1, Cela1, Chymotrypsin-like elastase family member 1, Elastase-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastase-1 antibody (AB231117)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastase-1 antibody (AB231117)

DAB stained, formalin-fixed, paraffin-embedded mouse testis tissue stained for Elastase-1 using ab231117 at 20 μg/mL in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Elastase-1 antibody (AB231117)

All lanes:

Western blot - Anti-Elastase-1 antibody (ab231117) at 1 µg/mL

All lanes:

Mouse serum

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-Elastase-1 antibody (AB231117)

All lanes:

Western blot - Anti-Elastase-1 antibody (ab231117) at 1 µg/mL

All lanes:

Recombinant mouse Elastase-1 protein.

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Mouse Cela1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q91X79

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Elastase-1 also known as ELA1 or pancreatic elastase functions as a serine protease. This enzyme with a molecular mass of approximately 29 kDa assists in breaking down elastin an important protein in the connective tissue. Elastase-1 primarily expresses in the pancreas and gets secreted into the duodenum where it plays an important role in digestion. Its specificity for insoluble elastin makes it distinct among proteolytic enzymes.

Biological function summary

Elastase-1 contributes significantly to digestive processes by degrading elastin and other proteins in the small intestine. This action prepares proteins for absorption into the body. Elastase-1 does not work alone; it acts as part of a complex group of pancreatic enzymes including trypsin and chymotrypsin which together facilitate complete protein digestion. These associated enzymes jointly ensure efficient breakdown of dietary components optimizing nutrient uptake.

Pathways

Elastase-1 participates in the digestion and absorption pathways of proteins. It functions alongside other digestive enzymes such as amylase and lipase which are essential in the breakdown of carbohydrates and fats. Elastase-1 has connections to pathways involved in proteolysis acting synergistically with enzymes like trypsin for effective protein digestion. These pathways highlight the cooperative nature of enzyme systems in the digestive tract.

Elastase-1 often becomes a focus in conditions such as chronic pancreatitis and cystic fibrosis. Reduced levels of elastase-1 in the stool serve as a marker for pancreatic insufficiency a condition where the pancreas fails to secrete adequate digestive enzymes. In cystic fibrosis thick mucus secretions can impede elastase-1 from reaching the duodenum exacerbating malabsorption issues. Proteins like trypsin and chymotrypsin can also be involved as impaired pancreatic enzyme excretion affects multiple enzyme systems complicating nutrient absorption and digestion.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine proteases that hydrolyze many proteins in addition to elastin.

See full target information Cela1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:640 PubMed39251573

2024

Mesenchymal stromal cells restrain the Th17 cell response via L-amino-acid oxidase within lymph nodes.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Ni,Le Zhen,Zhu Zeng,Jingwen Yang,Yukai Wang,Huanke Xu,Qixiang Zhang,Yongcheng Zhu,Yu Tao,Jing Wang,Qing Liu,Kezheng Yi,Yang Chen,Qian Chen,Guangji Wang,Fang Zhou,Yunlong Shan

International journal of molecular sciences 23: PubMed35743192

2022

Copper-Binding Domain Variation in a Novel Murine Lysyl Oxidase Model Produces Structurally Inferior Aortic Elastic Fibers Whose Failure Is Modified by Age, Sex, and Blood Pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Kit Man Tsang,Russell H Knutsen,Charles J Billington,Eric Lindberg,Heiko Steenbock,Yi-Ping Fu,Amanda Wardlaw-Pickett,Delong Liu,Daniela Malide,Zu-Xi Yu,Christopher K E Bleck,Jürgen Brinckmann,Beth A Kozel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Elastase-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastase-1 antibody (AB231117)

Western blot - Anti-Elastase-1 antibody (AB231117)

Western blot - Anti-Elastase-1 antibody (AB231117)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise