JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB212377

Anti-Elastase antibody [CELA3B/1218] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal CEL3B antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CELA3B.

View Alternative Names

ELA3B, CELA3B, Chymotrypsin-like elastase family member 3B, Elastase IIIB, Elastase-3B, Protease E

1 Images
Western blot - Anti-Elastase antibody [CELA3B/1218] - BSA and Azide free (AB212377)
  • WB

Supplier Data

Western blot - Anti-Elastase antibody [CELA3B/1218] - BSA and Azide free (AB212377)

All lanes:

Western blot - Anti-Elastase antibody [CELA3B/1218] - BSA and Azide free (ab212377)

Lane 1:

Recombinant Cela3b

Lane 2:

Panc28 cell lysate

Lane 3:

PANC1 cell lysate

Predicted band size: 29 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CELA3B/1218

Isotype

IgG

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CELA3B.

P08861

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Elastase also known as CEL-A3B refers to a family of serine proteases with an approximate mass of 29 kDa. It primarily resides in the pancreas and neutrophils where it performs key enzymatic roles by breaking down elastin and other proteins. Humans express elastase in various forms including pancreatic elastase and neutrophil elastase. In these tissues elastase also participates in immune defense and proteolysis of extracellular matrix components.
Biological function summary

Elastase serves multiple functions beyond simple protein degradation. As a component in various complexes such as those found in neutrophils elastase helps in modulating inflammatory responses. It cleaves secreted factors and membrane proteins affecting cell signaling and recruiting additional immune cells. This protease also regulates tissue remodeling by degrading unwanted matrix proteins facilitating cellular migration and healing processes.

Pathways

Elastase significantly impacts inflammatory and tissue repair pathways. It interacts with proteins such as alpha-1 antitrypsin which serves as a natural inhibitor helping to control elastase activity. In particular elastase plays a role in the inflammatory response pathway breaking down cytokines and chemokines and is also implicated in fibronectin degradation part of the tissue repair process. Through these interactions elastase helps to modulate the balance between proteolysis and tissue preservation.

Elastase exhibits a notable connection to conditions like chronic obstructive pulmonary disease (COPD) and cystic fibrosis. Overactivity or misregulation of elastase can lead to destructure of lung tissue contributing to COPD. In cystic fibrosis mucus buildup leads to heightened inflammation and tissue damage partly due to unregulated elastase activity. Both diseases show interactions with proteins like alpha-1 antitrypsin where deficiencies further exacerbate elastase's pathological effects highlighting its clinical importance.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Efficient protease with alanine specificity but only little elastolytic activity.
See full target information CELA3B
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 7:47650-47662 PubMed27351281

2016

Obesity does not promote tumorigenesis of localized patient-derived prostate cancer xenografts.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer C Y Lo,Ashlee K Clark,Natasha Ascui,Mark Frydenberg,Gail P Risbridger,Renea A Taylor,Matthew J Watt
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB21590

Anti-Elastase antibody

  • 0
  • 0

Complementary Products

Primary Antibodies

AB232708

Anti-Elastase 3A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastase 3A antibody (AB232708)

  • 0
  • 0
Primary Antibodies

AB68672

Anti-Neutrophil Elastase antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody (AB68672)

  • 4
  • 20
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB208670

Anti-Myeloperoxidase antibody [EPR20257]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

  • 5
  • 7

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com