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AB77804

Anti-Elastin antibody [10B8]

4

(6 Reviews)

|

(9 Publications)

Mouse Monoclonal Elastin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 9 publications.

View Alternative Names

Elastin, Tropoelastin, ELN

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [10B8] (AB77804)
  • IHC-P

AbReview28499****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [10B8] (AB77804)

ab77804 staining dog lung sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes, followed by staining with ab77804 at 1/100 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Carl Hobbs

  • Carrier free

    Anti-Elastin antibody [10B8] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10B8

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Goat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Elastin also known as elastin protein is an essential component of the extracellular matrix known for imparting elasticity and resilience to tissues. With a molecular mass of approximately 60-70 kDa some scientists may also refer to it as elastin et. Elastin is expressed in elastic tissues such as the skin lungs and blood vessels. These tissues require the elastic properties provided by elastin. Additionally elastin forms the core of elastic fibers surrounded by a sheath of microfibrils.
Biological function summary

Elastin provides mechanical support and elasticity to tissues. It does so by being part of elastic fibers playing an important role in many connective tissue structures. The unique ability to stretch and return to its original shape allows tissues to resume their shape after stretching or contracting. Elastin is not part of larger protein complexes but interacts with other matrix proteins such as fibrillin to form a fibrous network required for the structural integrity of elastic tissues.

Pathways

Elastin plays an integral role in the extracellular matrix remodeling pathway which is important for maintaining tissue structure and function. It also participates in the TGF-beta signaling pathway where elastin interacts indirectly with proteins such as fibrillin-1 and fibulin. These interactions influence cellular processes like proliferation and differentiation highlighting elastin's involvement in both maintaining tissue elasticity and cell signaling.

Elastin is linked to pathologies like Williams-Beuren syndrome and cutis laxa. Williams-Beuren syndrome associated with elastin deletions leads to cardiovascular issues while cutis laxa results in reduced elasticity of the skin due to elastin mutations. In these conditions elastin interacts with proteins such as fibrillin and fibulin contributing to the observed clinical manifestations. Understanding these interactions helps in diagnosing and developing therapeutic approaches for such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely.
See full target information ELN
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:18296 PubMed40419589

2025

Comparison of the effects of fractional microneedle radiofrequency and microneedling on modulating the senescent fibroblast milieu in aged skin.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Min Hwang,Soo Hyun Lee,Eun Jae Baek,Hye-Rin Charlotte Kim,Jang-Hee Oh,Ji Su Lee,Si-Hyung Lee

Frontiers in physiology 13:924908 PubMed35733984

2022

Cerebral Blood Flow Autoregulation in Offspring From Experimentally Preeclamptic Rats and the Effect of Age.

Applications

Unspecified application

Species

Unspecified reactive species

Emmett E Whitaker,Abbie C Johnson,Sarah M Tremble,Conor McGinn,Nicole DeLance,Marilyn J Cipolla

Journal of cosmetic dermatology 21:4796-4804 PubMed35064622

2022

Elastin microfibril interface-located protein 1 and its catabolic enzyme, cathepsin K, regulate the age-related structure of elastic fibers in the skin.

Applications

Unspecified application

Species

Unspecified reactive species

Shinya Kondo,Takeshi Tohgasaki,Soichiro Shiga,Shino Nishizawa,Shioji Ishiwatari,Shinsuke Ishikawa,Akira Takeda,Tetsuhito Sakurai

Asian Pacific journal of allergy and immunology : PubMed34246206

2021

Elevation of anti-elastin antibody in patients with asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Shingo Tokita,Kumiya Sugiyama,Tomoshige Wakayama,Hajime Arifuku,Naotatsu Otsuji,Kei Sugitate,Takayoshi Owada,Kenya Koyama,Hirokuni Hirata,Masafumi Arima,Yoshihiko Ueda,Yasutsugu Fukushima

Frontiers in cell and developmental biology 8:621016 PubMed33537312

2021

Vascular Adhesion Protein-1 Determines the Cellular Properties of Endometrial Pericytes.

Applications

Unspecified application

Species

Unspecified reactive species

Seley Gharanei,Katherine Fishwick,Ruban Peter Durairaj,Tianrong Jin,Eleftherios Siamantouras,Kuo-Kang Liu,Anne Straube,Emma S Lucas,Christopher J Weston,Pia Rantakari,Marko Salmi,Sirpa Jalkanen,Jan J Brosens,Bee Kang Tan

Developmental biology 463:26-38 PubMed32151560

2020

Desert hedgehog-primary cilia cross talk shapes mitral valve tissue by organizing smooth muscle actin.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Fulmer,Katelynn A Toomer,Janiece Glover,Lilong Guo,Kelsey Moore,Reece Moore,Rebecca Stairley,Cortney Gensemer,Sameer Abrol,Mary Kate Rumph,Faith Emetu,Joshua H Lipschutz,Colin McDowell,Justin Bian,Christina Wang,Tyler Beck,Andy Wessels,Marie-Ange Renault,Russell A Norris

Experimental dermatology 28:914-921 PubMed29957839

2018

Physical properties of the photodamaged human skin dermis: Rougher collagen surface and stiffer/harder mechanical properties.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Shao,Zhaoping Qin,James Alexander Wilks,Rebecca Mutesi Balimunkwe,Gary J Fisher,John J Voorhees,Taihao Quan

Circulation research 116:23-34 PubMed25323858

2014

MicroRNA miR145 regulates TGFBR2 expression and matrix synthesis in vascular smooth muscle cells.

Applications

WB

Species

Unspecified reactive species

Ning Zhao,Sara N Koenig,Aaron J Trask,Cho-Hao Lin,Chetan P Hans,Vidu Garg,Brenda Lilly

Cardiovascular research 97:321-30 PubMed23060134

2012

Derivation and maturation of synthetic and contractile vascular smooth muscle cells from human pluripotent stem cells.

Applications

ICC/IF

Species

Human

Maureen Wanjare,Frederick Kuo,Sharon Gerecht
View all publications
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Product promise

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