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AB269450

Anti-Elastin antibody [BA-4] - BSA and Azide free

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Mouse Recombinant Monoclonal Elastin antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Elastin, Tropoelastin, ELN

1 Images
Western blot - Anti-Elastin antibody [BA-4] - BSA and Azide free (AB269450)
  • WB

Supplier Data

Western blot - Anti-Elastin antibody [BA-4] - BSA and Azide free (AB269450)

Blocking and dilution buffer : 5% NFDM/TBST

Exposure time :

Lane 1 : 15 seconds

Lane 2 : 7.75 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab9519).

All lanes:

Western blot - Anti-Elastin antibody [BA-4] - BSA and Azide free (ab269450) at 0.51 µg/mL

Lane 1:

Human artery tissue lysate at 20 µg

Lane 2:

Human skin tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BA-4

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

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Reactivity data

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Product details

ab269450 is the carrier-free version of ab9519.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Elastin also known as elastin protein is an essential component of the extracellular matrix known for imparting elasticity and resilience to tissues. With a molecular mass of approximately 60-70 kDa some scientists may also refer to it as elastin et. Elastin is expressed in elastic tissues such as the skin lungs and blood vessels. These tissues require the elastic properties provided by elastin. Additionally elastin forms the core of elastic fibers surrounded by a sheath of microfibrils.
Biological function summary

Elastin provides mechanical support and elasticity to tissues. It does so by being part of elastic fibers playing an important role in many connective tissue structures. The unique ability to stretch and return to its original shape allows tissues to resume their shape after stretching or contracting. Elastin is not part of larger protein complexes but interacts with other matrix proteins such as fibrillin to form a fibrous network required for the structural integrity of elastic tissues.

Pathways

Elastin plays an integral role in the extracellular matrix remodeling pathway which is important for maintaining tissue structure and function. It also participates in the TGF-beta signaling pathway where elastin interacts indirectly with proteins such as fibrillin-1 and fibulin. These interactions influence cellular processes like proliferation and differentiation highlighting elastin's involvement in both maintaining tissue elasticity and cell signaling.

Elastin is linked to pathologies like Williams-Beuren syndrome and cutis laxa. Williams-Beuren syndrome associated with elastin deletions leads to cardiovascular issues while cutis laxa results in reduced elasticity of the skin due to elastin mutations. In these conditions elastin interacts with proteins such as fibrillin and fibulin contributing to the observed clinical manifestations. Understanding these interactions helps in diagnosing and developing therapeutic approaches for such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).
See full target information ELN
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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