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Rabbit Recombinant Monoclonal Elastin antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications.


Images

Western blot - Anti-Elastin antibody [EPR20603] (AB213720), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (AB213720), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (AB213720), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (AB213720), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (AB213720), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).

Alternative names

Elastin, Tropoelastin, ELN

Recommended products

Rabbit Recombinant Monoclonal Elastin antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications.

Alternative names

Elastin, Tropoelastin, ELN

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR20603

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Elastin also known as elastin protein is an essential component of the extracellular matrix known for imparting elasticity and resilience to tissues. With a molecular mass of approximately 60-70 kDa some scientists may also refer to it as elastin et. Elastin is expressed in elastic tissues such as the skin lungs and blood vessels. These tissues require the elastic properties provided by elastin. Additionally elastin forms the core of elastic fibers surrounded by a sheath of microfibrils.

Biological function summary

Elastin provides mechanical support and elasticity to tissues. It does so by being part of elastic fibers playing an important role in many connective tissue structures. The unique ability to stretch and return to its original shape allows tissues to resume their shape after stretching or contracting. Elastin is not part of larger protein complexes but interacts with other matrix proteins such as fibrillin to form a fibrous network required for the structural integrity of elastic tissues.

Pathways

Elastin plays an integral role in the extracellular matrix remodeling pathway which is important for maintaining tissue structure and function. It also participates in the TGF-beta signaling pathway where elastin interacts indirectly with proteins such as fibrillin-1 and fibulin. These interactions influence cellular processes like proliferation and differentiation highlighting elastin's involvement in both maintaining tissue elasticity and cell signaling.

Associated diseases and disorders

Elastin is linked to pathologies like Williams-Beuren syndrome and cutis laxa. Williams-Beuren syndrome associated with elastin deletions leads to cardiovascular issues while cutis laxa results in reduced elasticity of the skin due to elastin mutations. In these conditions elastin interacts with proteins such as fibrillin and fibulin contributing to the observed clinical manifestations. Understanding these interactions helps in diagnosing and developing therapeutic approaches for such disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Elastin antibody [EPR20603] (ab213720), expandable thumbnail

    Western blot - Anti-Elastin antibody [EPR20603] (ab213720)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Elastin antibody [EPR20603] (ab213720) at 1/1000 dilution

    All lanes: Human artery lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/10000 dilution

    Predicted band size: 68 kDa

    Observed band size: 68 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720)

    Immunohistochemical analysis of paraffin-embedded blood vessels in human lung tissue labeling Elastin with ab213720 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on blood vessels in human lung [PMID: 24461327].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Elastin with ab213720 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on smooth muscle cells of human testis.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720)

    Immunohistochemical analysis of paraffin-embedded artery in human liver tissue labeling Elastin with ab213720 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular staining on the artery in human liver [PMID: 24461327].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma labeling Elastin with ab213720 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880) Ready to use. Positive staining on vascular smooth muscle cells of human breast carcinoma.

    Counter stained with Hematoxylin.

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0) before commencing with IHC staining protocol. The section was incubated with ab213720 at 4°C overnight.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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