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Anti-Elastin antibody [EPR20603] (ab213720) is a rabbit monoclonal antibody detecting Elastin in Western Blot, IHC-P. Suitable for Human.



- Biophysical QC for unrivalled batch-batch consistency


Images

Western blot - Anti-Elastin antibody [EPR20603] (AB213720), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (AB213720), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (AB213720), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (AB213720), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (AB213720), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).

Alternative names

Elastin, Tropoelastin, ELN

Recommended products

Anti-Elastin antibody [EPR20603] (ab213720) is a rabbit monoclonal antibody detecting Elastin in Western Blot, IHC-P. Suitable for Human.



- Biophysical QC for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20603
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Elastin antibody [EPR20603] (ab213720) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Elastin?


Anti-Elastin [EPR20603] (ab213720) specifically detects a band for Elastin (UniProt: P15502) at a molecular weight of 68kDa.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [EPR20603] also available for your convenience:
ab213720, Carrier free - Anti-Elastin antibody [EPR20603] - BSA and Azide free ab238981, PE - PE Anti-Elastin antibody [EPR20603] ab318355, APC - APC Anti-Elastin antibody [EPR20603] ab318458, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Elastin antibody [EPR20603] ab318561, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Elastin antibody [EPR20603] ab318664, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Elastin antibody [EPR20603] ab318767, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Elastin antibody [EPR20603] ab318867, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-Elastin antibody [EPR20603] ab321375



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Elastin also known as elastin protein is an essential component of the extracellular matrix known for imparting elasticity and resilience to tissues. With a molecular mass of approximately 60-70 kDa some scientists may also refer to it as elastin et. Elastin is expressed in elastic tissues such as the skin lungs and blood vessels. These tissues require the elastic properties provided by elastin. Additionally elastin forms the core of elastic fibers surrounded by a sheath of microfibrils.

Biological function summary

Elastin provides mechanical support and elasticity to tissues. It does so by being part of elastic fibers playing an important role in many connective tissue structures. The unique ability to stretch and return to its original shape allows tissues to resume their shape after stretching or contracting. Elastin is not part of larger protein complexes but interacts with other matrix proteins such as fibrillin to form a fibrous network required for the structural integrity of elastic tissues.

Pathways

Elastin plays an integral role in the extracellular matrix remodeling pathway which is important for maintaining tissue structure and function. It also participates in the TGF-beta signaling pathway where elastin interacts indirectly with proteins such as fibrillin-1 and fibulin. These interactions influence cellular processes like proliferation and differentiation highlighting elastin's involvement in both maintaining tissue elasticity and cell signaling.

Associated diseases and disorders

Elastin is linked to pathologies like Williams-Beuren syndrome and cutis laxa. Williams-Beuren syndrome associated with elastin deletions leads to cardiovascular issues while cutis laxa results in reduced elasticity of the skin due to elastin mutations. In these conditions elastin interacts with proteins such as fibrillin and fibulin contributing to the observed clinical manifestations. Understanding these interactions helps in diagnosing and developing therapeutic approaches for such disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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6 product images

  • Western blot - Anti-Elastin antibody [EPR20603] (ab213720), expandable thumbnail

    Western blot - Anti-Elastin antibody [EPR20603] (ab213720)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Elastin antibody [EPR20603] (ab213720) at 1/1000 dilution

    All lanes: Human artery lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/10000 dilution

    Predicted band size: 68 kDa

    Observed band size: 68 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720)

    Immunohistochemical analysis of paraffin-embedded blood vessels in human lung tissue labeling Elastin with ab213720 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on blood vessels in human lung [PMID: 24461327].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Elastin with ab213720 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on smooth muscle cells of human testis.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720)

    Immunohistochemical analysis of paraffin-embedded artery in human liver tissue labeling Elastin with ab213720 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular staining on the artery in human liver [PMID: 24461327].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] (ab213720)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma labeling Elastin with ab213720 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880) Ready to use. Positive staining on vascular smooth muscle cells of human breast carcinoma.

    Counter stained with Hematoxylin.

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0) before commencing with IHC staining protocol. The section was incubated with ab213720 at 4°C overnight.

  • Western blot - Anti-Elastin antibody [EPR20603] (ab213720), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-Elastin antibody [EPR20603] (ab213720)

    Elastin western blot using anti-Elastin antibody [EPR20603] ab213720. Publication image and figure legend from Ren, S., Chen, J., et al., 2019, Stem Cell Res Ther, PubMed 30704535.


    ab213720 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab213720 please see the product overview.

    Effect of ASC-MV treatment on gene expression in all three kinds of cells. qRT-PCR analysis of a cluster of gene expression in cells treated with either PBS or 20 μg/ml ASC-MVs. a Upregulated genes in HUVECs. c Upregulated genes in HaCAT. e Upregulated genes in fibroblasts. Western blot analysis of gene expression in cells given above treatments. b Upregulated genes in HUVECs. d HaCAT. f Fibroblasts. GAPDH served as an internal control. N = 3. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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Product protocols

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