Rabbit Recombinant Monoclonal Elastin antibody. Carrier free. Suitable for IHC-P, WB, IHC-Fr and reacts with Mouse, Rat samples. Cited in 1 publication.
Images
![Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (AB307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-frozen-sections-img405014.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (AB307151)")
![Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (AB307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-frozen-sections-img405013.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (AB307151)")
![Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (AB307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-img405020.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (AB307151)")
![Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (AB307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-img405018.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (AB307151)")
![Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (AB307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-img405017.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (AB307151)")
Publications
Filter by
- Frontiers in bioengineering and biotechnology 11:11249442023Gallium-modified gelatin nanoparticles loaded with quercetin promote skin wound healing the regulation of bacterial proliferation and macrophage polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNing Yang,Nianyuan Shi,Zhou Yao,Hang Liu,Weinan GuoPubMed 36777248
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC-P | WB | IHC-Fr | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Tested | Tested |
| Rat | Not recommended | Tested | Tested | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
Alternative names
Elastin, Tropoelastin, Eln
Recommended products
Rabbit Recombinant Monoclonal Elastin antibody. Carrier free. Suitable for IHC-P, WB, IHC-Fr and reacts with Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26834-89
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Elastin also known as elastin protein is an essential component of the extracellular matrix known for imparting elasticity and resilience to tissues. With a molecular mass of approximately 60-70 kDa some scientists may also refer to it as elastin et. Elastin is expressed in elastic tissues such as the skin lungs and blood vessels. These tissues require the elastic properties provided by elastin. Additionally elastin forms the core of elastic fibers surrounded by a sheath of microfibrils.
- Biological function summary
Elastin provides mechanical support and elasticity to tissues. It does so by being part of elastic fibers playing an important role in many connective tissue structures. The unique ability to stretch and return to its original shape allows tissues to resume their shape after stretching or contracting. Elastin is not part of larger protein complexes but interacts with other matrix proteins such as fibrillin to form a fibrous network required for the structural integrity of elastic tissues.
- Pathways
Elastin plays an integral role in the extracellular matrix remodeling pathway which is important for maintaining tissue structure and function. It also participates in the TGF-beta signaling pathway where elastin interacts indirectly with proteins such as fibrillin-1 and fibulin. These interactions influence cellular processes like proliferation and differentiation highlighting elastin's involvement in both maintaining tissue elasticity and cell signaling.
- Associated diseases and disorders
Elastin is linked to pathologies like Williams-Beuren syndrome and cutis laxa. Williams-Beuren syndrome associated with elastin deletions leads to cardiovascular issues while cutis laxa results in reduced elasticity of the skin due to elastin mutations. In these conditions elastin interacts with proteins such as fibrillin and fibulin contributing to the observed clinical manifestations. Understanding these interactions helps in diagnosing and developing therapeutic approaches for such disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-frozen-sections-img405014.jpg "Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)")
Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)
This data was developed using Anti-Elastin antibody [EPR26834-89] ab307150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung (fresh) tissue labeling Elastin with Anti-Elastin antibody [EPR26834-89] ab307150 at 1/100 dilution (4.99 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
Confocal image showing positive staining on mouse lung.
The section was incubated with Anti-Elastin antibody [EPR26834-89] ab307150 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).![Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-frozen-sections-img405013.jpg "Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)")
Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)
This data was developed using Anti-Elastin antibody [EPR26834-89] ab307150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling Elastin with Anti-Elastin antibody [EPR26834-89] ab307150 at 1/100 dilution (4.99 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
Negative control: confocal image showing no staining on mouse cerebrum (PMID: 25166724).
The section was incubated with Anti-Elastin antibody [EPR26834-89] ab307150 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).![Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-img405020.jpg "Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)")
Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)
This data was developed using Anti-Elastin antibody [EPR26834-89] ab307150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Elastin with Anti-Elastin antibody [EPR26834-89] ab307150 at 1/2000 (0.25 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on extracellular matrix of mouse testis. The section was incubated with Anti-Elastin antibody [EPR26834-89] ab307150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-img405018.jpg "Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)")
Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)
This data was developed using Anti-Elastin antibody [EPR26834-89] ab307150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver carcinoma tissue labeling Elastin with Anti-Elastin antibody [EPR26834-89] ab307150 at 1/2000 (0.25 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on extracellular matrix of mouse liver carcinoma. The section was incubated with Anti-Elastin antibody [EPR26834-89] ab307150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-img405017.jpg "Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)")
Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)
This data was developed using Anti-Elastin antibody [EPR26834-89] ab307150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Elastin with Anti-Elastin antibody [EPR26834-89] ab307150 at 1/2000 (0.25 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Negative control: No staining on mouse cerebrum.
The section was incubated with Anti-Elastin antibody [EPR26834-89] ab307150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-frozen-sections-img405016.jpg "Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)")
Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)
This data was developed using Anti-Elastin antibody [EPR26834-89] ab307150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat lung (fresh) tissue labeling Elastin with Anti-Elastin antibody [EPR26834-89] ab307150 at 1/100 dilution (4.99 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
Confocal image showing positive staining on rat lung.
The section was incubated with Anti-Elastin antibody [EPR26834-89] ab307150 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution (2 ug/mL).![Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-img405021.jpg "Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)")
Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)
This data was developed using Anti-Elastin antibody [EPR26834-89] ab307150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Elastin with Anti-Elastin antibody [EPR26834-89] ab307150 at 1/2000 (0.25 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat lung (PMID: 29844468). The section was incubated with Anti-Elastin antibody [EPR26834-89] ab307150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Western blot - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--western-blot-img405022.jpg "Western blot - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)")
Western blot - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)
This data was developed using Anti-Elastin antibody [EPR26834-89] ab307150, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: mouse brain (PMID:14794650).
Exposure time: 92 seconds.All lanes: Western blot - Anti-Elastin antibody [EPR26834-89] (Anti-Elastin antibody [EPR26834-89] ab307150) at 1/1000 dilution
Lane 1: Mouse artery tissue lysate 20 μg
Lane 2: Mouse brain tissue lysate 20 μg
Lane 3: Rat lung tissue lysate 20 μg
Lane 4: Rat testis tissue lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 68 kDa
Exposure time: 92s
![Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-img405019.jpg "Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)")
Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)
This data was developed using Anti-Elastin antibody [EPR26834-89] ab307150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Elastin with Anti-Elastin antibody [EPR26834-89] ab307150 at 1/2000 (0.25 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse lung (PMID: 29844468). The section was incubated with Anti-Elastin antibody [EPR26834-89] ab307150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-frozen-sections-img405015.jpg "Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)")
Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)
This data was developed using Anti-Elastin antibody [EPR26834-89] ab307150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling Elastin with Anti-Elastin antibody [EPR26834-89] ab307150 at 1/100 dilution (4.99 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
Negative control: confocal image showing no staining on rat cerebrum (PMID: 25166724).
The section was incubated with Anti-Elastin antibody [EPR26834-89] ab307150 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).
![Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-frozen-sections-img405016.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)")
![Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-img405021.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)")
![Western blot - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--western-blot-img405022.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)")
![Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-img405019.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)")
![Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151), expandable thumbnail](https://content.abcam.com/products/images/elastin-antibody-epr26834-89-bsa-and-azide-free-ab307151--immunohistochemistry-frozen-sections-img405015.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Elastin antibody [EPR26834-89] - BSA and Azide free (ab307151)")
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