AB327161

Anti-ELAVL2 Antibody [CL15323]

Name: Anti-ELAVL2 Antibody [CL15323] (ab327161) | Abcam
Brand: Abcam Limited
SKU: AB327161
Price: 600 USD
Availability: InStock

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Mouse Monoclonal ELAVL2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human ELAVL2.

View Alternative Names

HUB, ELAVL2, ELAV-like protein 2, ELAV-like neuronal protein 1, Hu-antigen B, Nervous system-specific RNA-binding protein Hel-N1, HuB

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAVL2 Antibody [CL15323] (AB327161)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAVL2 Antibody [CL15323] (AB327161)

Immunohistochemical staining of human cerebellum using ab327161 at 1 : 1000–1 : 2500 dilution, showing strong cytoplasmic positivity in a subset of neurons in the granular layer.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAVL2 Antibody [CL15323] (AB327161)

Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using ab327161 at 1 : 1000–1 : 2500 dilution. Corresponding ELAVL2 RNA-seq data are presented for the same tissues.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAVL2 Antibody [CL15323] (AB327161)

Immunohistochemical staining of human testis using ab327161 at 1 : 1000–1 : 2500 dilution, showing strong cytoplasmic positivity in a subset of cells in seminiferous ducts.

Immunocytochemistry/ Immunofluorescence - Anti-ELAVL2 Antibody [CL15323] (AB327161)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ELAVL2 Antibody [CL15323] (AB327161)

Immunofluorescence staining in A-431 cell line using ab327161 at 2–10 µg/ml, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAVL2 Antibody [CL15323] (AB327161)

Immunohistochemical staining of rat hippocampus using ab327161 at 1 : 1000–1 : 2500 dilution, showing strong cytoplasmic positivity in the dentate gyrus neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAVL2 Antibody [CL15323] (AB327161)

Immunohistochemical staining of mouse cerebral cortex using ab327161 at 1 : 1000–1 : 2500 dilution, showing strong cytoplasmic positivity in neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAVL2 Antibody [CL15323] (AB327161)

Immunohistochemical staining of rat cerebral cortex using ab327161 at 1 : 1000–1 : 2500 dilution, showing strong cytoplasmic positivity in a subset of neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELAVL2 Antibody [CL15323] (AB327161)

Immunohistochemical staining of human skeletal muscle using ab327161 at 1 : 1000–1 : 2500 dilution, showing no positivity in myocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL15323

Isotype

IgG3

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human ELAVL2.

Q12926

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein that binds to the 3' untranslated region (3'UTR) of target mRNAs (By similarity). Seems to recognize a GAAA motif (By similarity). Can bind to its own 3'UTR, the FOS 3'UTR and the ID 3'UTR (By similarity).

See full target information ELAVL2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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