JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB308577

Anti-ELAVL2 antibody [HL1978]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal ELAVL2 antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human ELAVL2.

View Alternative Names

HUB, ELAVL2, ELAV-like protein 2, ELAV-like neuronal protein 1, Hu-antigen B, Nervous system-specific RNA-binding protein Hel-N1, HuB

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ELAVL2 antibody [HL1978] (AB308577)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ELAVL2 antibody [HL1978] (AB308577)

For immunofluorescence analysis, A431 cells were fixed in ice-cold MeOH for 5 minutes and permeabilized for detection of ELAVL2 protein using ab308577 at 1/500 (green). Blue : Fluoroshield with DAPI.

Immunohistochemistry paraffin embedded sections - Anti-ELAVL2 antibody [HL1978] (AB308577)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-ELAVL2 antibody [HL1978] (AB308577)

Immunohistochemical analysis of paraffin-embedded mouse brain and urinary bladder tissues labeling ELAVL2 protein with ab308577 at 1/100. Antigen retrieval was performed using Citrate buffer, pH 6.0, 15 min

Immunohistochemistry paraffin embedded sections - Anti-ELAVL2 antibody [HL1978] (AB308577)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-ELAVL2 antibody [HL1978] (AB308577)

Immunohistochemical analysis of paraffin-embedded rat testis and brain tissues labeling ELAVL2 protein with ab308577 at 1/100. Antigen retrieval was performed using Citrate buffer, pH 6.0, 15 min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1978

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ELAVL2. The exact immunogen used to generate this antibody is proprietary information.

Q12926

style
left-column

Complementary Products

Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 74
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELAVL2 also known as HuB or Hel-N1 is an RNA-binding protein with a molecular weight of approximately 39 kDa. ELAVL2 binds to AU-rich elements (AREs) in the 3' untranslated regions (UTRs) of target mRNAs which affects mRNA stability and translation. The expression of ELAVL2 occurs mainly in the nervous system particularly in neurons where it regulates neuronal differentiation and function. Its precise expression in neural tissues suggests it plays a role in neurodevelopment.
Biological function summary

ELAVL2 modulates the post-transcriptional regulation of mRNA influencing processes like mRNA localization stabilization and translation within neurons. It forms part of ribonucleoprotein complexes that manage the life cycle of mRNAs. Through its interaction with specific mRNA targets ELAVL2 affects the translation of proteins that are essential for synaptic plasticity and neuronal connectivity which are critical processes in maintaining healthy brain function.

Pathways

ELAVL2 integrates into pathways that include the regulation of neuronal mRNA surveillance and synaptic signaling. In these pathways ELAVL2 interacts with related proteins such as ELAVL1 and ELAVL3 which also bind to RNA. These proteins share a common function in maintaining the balance of mRNA levels within neurons. ELAVL2's role in synaptic signaling highlights its involvement in the transmission of information across synapses important for learning and memory.

ELAVL2 has associations with neurological conditions like Alzheimer's disease and autism spectrum disorders. Studies indicate its altered regulation can affect mRNA metabolism in neurons potentially contributing to these disorders. ELAVL2's interaction with proteins such as Tau in Alzheimer's disease or various mRNA targets in developmental disorders reveals its significant position in the pathology of neural conditions where it alters post-transcriptional gene regulation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

RNA-binding protein that binds to the 3' untranslated region (3'UTR) of target mRNAs (By similarity). Seems to recognize a GAAA motif (By similarity). Can bind to its own 3'UTR, the FOS 3'UTR and the ID 3'UTR (By similarity).
See full target information ELAVL2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com