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AB64937

Anti-ELF1 antibody

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(4 Publications)

Rabbit Polyclonal ELF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ELF1.

View Alternative Names

ETS-related transcription factor Elf-1, E74-like factor 1, ELF1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ELF1 antibody (AB64937)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ELF1 antibody (AB64937)

This image shows A549 cells stained with ab64937 at 1/500 dilution; with and without the immunising peptide.

Western blot - Anti-ELF1 antibody (AB64937)
  • WB

Unknown

Western blot - Anti-ELF1 antibody (AB64937)

All lanes:

Western blot - Anti-ELF1 antibody (ab64937) at 1/500 dilution

Lane 1:

extracts from 293 cells (5-30µg total protein)

Lane 2:

extracts from HuvEc cells (5-30µg total protein)

Lane 3:

extracts from HuvEc cells (5-30µg total protein) and 5-10µg of the immunising peptide.

Predicted band size: 67 kDa

Observed band size: 67 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human ELF1. The exact immunogen used to generate this antibody is proprietary information.

P32519

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ELF1 (E74 Like ETS Transcription Factor 1) protein serves as a transcription factor involved in regulating gene expression. It is also known as E74-like factor 1 or ELF-1. This protein has a molecular mass of approximately 68 kDa and is expressed in various tissues with high levels observed in the thymus and peripheral blood leukocytes. ELF1 plays a role in activating or repressing specific genes by binding to DNA sequences in their promoter regions. This transcriptional activity influences cell growth differentiation and other vital cellular processes.
Biological function summary

ELF1 plays an important role in immune cell function and development. It operates as part of the ETS family of transcription factors which form complexes with other proteins to exert their effects on gene expression. ELF1 contributes to the regulation of T-cell development and function an essential process in maintaining efficient immune responses. The protein also influences other cellular processes including hematopoiesis and lymphoid organogenesis due to its target genes.

Pathways

ELF1 participates in immune system pathways including those governing T-cell activation and differentiation. These pathways often involve synergy with related proteins such as ELK1 and ETS1 which also belong to the ETS transcription factor family. These proteins exhibit interactions that modulate cellular responses during immune signaling. ELF1's regulatory influence helps maintain balance between proliferation and differentiation critical for immune system function.

ELF1 has links to certain autoimmune diseases like systemic lupus erythematosus (SLE) and hematological malignancies such as leukemia. Abnormal ELF1 expression or function may disrupt normal immune regulation contributing to autoimmune pathology. Moreover interactions with proteins like PAX5 in lymphoid cells can relate to oncogenic processes. Here ELF1's regulation can become dysregulated aiding in cancer progression or the onset of autoimmune conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.
See full target information ELF1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

ACS chemical neuroscience 13:2449-2463 PubMed35901231

2022

Furanoditerpenes from Display Mitochondrial-Mediated Neuroprotective Effects by Targeting Cyclophilin D.

Applications

Unspecified application

Species

Unspecified reactive species

Rebeca Alvariño,Amparo Alfonso,Dawrin Pech-Puch,Sandra Gegunde,Jaime Rodríguez,Mercedes R Vieytes,Carlos Jiménez,Luis M Botana

Cancer gene therapy 29:122-132 PubMed33531646

2021

SNHG17/miR-384/ELF1 axis promotes cell growth by transcriptional regulation of CTNNB1 to activate Wnt/β-catenin pathway in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Qiao,Tianyi Qiao,Shihui Yang,Lili Liu,Mengdan Zheng

Molecular therapy. Nucleic acids 23:418-430 PubMed33473327

2021

Transcription Factor ELF1 Activates MEIS1 Transcription and Then Regulates the GFI1/FBW7 Axis to Promote the Development of Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Meixiong Cheng,Yi Zeng,Tian Zhang,Min Xu,Zhili Li,Yaqiu Wu

Molecular immunology 111:209-219 PubMed31096062

2019

The mouse intron-nested gene, Israa, is expressed in the lymphoid organs and involved in T-cell activation and signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Noureddine Ben Khalaf,Wedad Al-Mashoor,Azhar Saeed,Dalal Al-Mehatab,Safa Taha,Moiz Bakhiet,M Dahmani Fathallah
View all publications
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