Rabbit Polyclonal EIF2S1 phospho S52 antibody. N-terminal. Suitable for WB and reacts with Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human EIF2S1 phospho S52.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
WB | |
---|---|
Human | Tested |
Rat | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed:16289705, PubMed:38340717). This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (43S PIC) (PubMed:16289705). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex (PubMed:16289705). In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (PubMed:16289705). EIF2S1/eIF2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed:19131336, PubMed:33384352, PubMed:38340717). EIF2S1/eIF2-alpha also acts as an activator of mitophagy in response to mitochondrial damage: phosphorylation by EIF2AK1/HRI promotes relocalization to the mitochondrial surface, thereby triggering PRKN-independent mitophagy (PubMed:38340717).
EIF2A, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, eIF2-alpha
Rabbit Polyclonal EIF2S1 phospho S52 antibody. N-terminal. Suitable for WB and reacts with Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human EIF2S1 phospho S52.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The eIF2 alpha also known as eukaryotic translation initiation factor 2 alpha is a subunit of the eIF2 complex with an approximate mass of 36 kDa. It plays an important role in protein synthesis initiation by facilitating the binding of the initiator methionyl-tRNA to the 40S ribosomal subunit. eIF2 alpha is widely expressed across various tissues indicating its fundamental role in cellular function. It is also regulated through phosphorylation as part of its function in modulation of translation initiation.
EIF2 alpha modulates protein synthesis in response to cellular stress signals. When phosphorylated eIF2 alpha inhibits the formation of the active eIF2-GTP-Met-tRNA complex reducing general protein translation and facilitating expression of stress-response genes. As part of the eIF2 complex it interacts with other proteins like eIF2β and eIF2γ highlighting its role in translation regulation and cellular response to various stimuli. This control mechanism is important for cellular adaptation to changing environmental conditions.
EIF2 alpha plays an important role in the integrated stress response (ISR) and the unfolded protein response (UPR). Phosphorylation of eIF2 alpha by kinases such as PERK and PKR links it to these pathways and demonstrates its key position in cellular stress response networks. eIF2 alpha's activity regulates downstream factors including activating transcription factor 4 (ATF4) which mediates the expression of genes involved in the stress response. This interconnectivity with key signaling pathways highlights its regulatory importance.
Dysregulation of eIF2 alpha phosphorylation links to various pathologies including neurodegenerative diseases and cancer. Aberrant eIF2 alpha activity can result in the misfolding of proteins contributing to diseases like Alzheimer's or lead to uncontrolled cell growth observed in certain cancers. In particular relationships between eIF2 alpha and proteins such as tau in neurodegeneration or MYC in cancer pathogenesis highlight its potential as a therapeutic target for these conditions.
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10% SDS-PAGE gel.
All lanes: Western blot - Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593) at 1/500 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 4 µg/ml tunicamycin for 16 hours, whole cell extract at 30 µg
Predicted band size: 36 kDa
10% SDS-PAGE gel.
All lanes: Western blot - Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593) at 1/500 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) treated with 3 µM thapsigargin for 12 hours, whole cell extract at 30 µg
Predicted band size: 36 kDa
10% SDS-PAGE gel.
All lanes: Western blot - Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593) at 1/1000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg
Lane 2: PC-12 (rat adrenal gland pheochromocytoma cell line) treated with 300 µM thapsigargin for 8 hours, whole cell extract at 30 µg
Predicted band size: 36 kDa
10% SDS-PAGE gel.
All lanes: Western blot - Anti-elF2 alpha (phospho S52) antibody - N-terminal (ab227593) at 1/500 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) treated with 25 µg/ml anisomycin for 16 hours, whole cell extract at 30 µg
Predicted band size: 36 kDa
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