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AB225958

Anti-ELF2/NERF antibody

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Rabbit Polyclonal ELF2/NERF antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ELF2 aa 1-150.

View Alternative Names

NERF, ELF2, ETS-related transcription factor Elf-2, E74-like factor 2, New ETS-related factor

3 Images
Western blot - Anti-ELF2/NERF antibody (AB225958)
  • WB

Supplier Data

Western blot - Anti-ELF2/NERF antibody (AB225958)

All lanes:

Western blot - Anti-ELF2/NERF antibody (ab225958) at 1/1000 dilution

Lane 1:

HL-60 (human promyelocytic leukemia cell line) whole cell lysate

Lane 2:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 3:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 5:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 64 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELF2/NERF antibody (AB225958)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELF2/NERF antibody (AB225958)

Paraffin embedded human skin tissue stained for ELF2/NERF with ab225958 (1/100 dilution) in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELF2/NERF antibody (AB225958)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELF2/NERF antibody (AB225958)

Paraffin embedded human placenta tissue stained for ELF2/NERF with ab225958 (1/100 dilution) in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ELF2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q15723

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ELF2/NERF protein also known as ELF2 or anti-NERF is a transcription factor involved in regulating gene expression. This protein has a molecular mass of approximately 65 kDa. ELF2/NERF is expressed in various tissue types including hematopoietic cells and is part of the ETS family of transcription factors know for its DNA-binding properties. Its function is modulated through specific DNA sequences where it promotes or represses transcription.
Biological function summary

ELF2/NERF plays a role in the control of immune response and hematopoietic cell differentiation. The protein is part of a transcriptional complex that interacts with other transcription factors such as GATA proteins. This interaction influences expression of target genes involved in immune response and blood cell formation. Through such actions ELF2/NERF impacts cell lineage commitment and activation.

Pathways

ELF2/NERF is involved in pathways integral to cell growth and development. It's particularly significant in the JAK-STAT signaling pathway which governs cellular proliferation differentiation and apoptosis. In this context ELF2/NERF frequently interacts with proteins like STAT5 which mediates signaling from various cytokines and growth factors ensuring proper cellular responses to external stimuli.

Dysregulation of ELF2/NERF is associated with conditions such as leukemia and autoimmune disorders. In leukemia abnormalities in ELF2/NERF expression affect the regulation of hematopoietic cell differentiation contributing to the uncontrolled proliferation of blood cells. Additionally interactions with other proteins like RUNX1 in blood malignancies influence disease progression highlighting the relevance of ELF2/NERF in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1 transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter.. Isoform 2 may function in repression of RUNX1-mediated transactivation.
See full target information ELF2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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