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AB222251

Anti-ELF5 antibody

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(2 Publications)

Rabbit Polyclonal ELF5 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ELF5 aa 150-250.

View Alternative Names

ESE2, ELF5, ETS-related transcription factor Elf-5, E74-like factor 5, Epithelium-restricted ESE-1-related Ets factor, Epithelium-specific Ets transcription factor 2, ESE-2

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-ELF5 antibody (AB222251)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ELF5 antibody (AB222251)

4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RT4 (human urinary bladder cancer cell line) cells stained for ELF5 (green) using ab222251 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ELF5 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q9UKW6

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Primary Antibodies

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Secondary Antibodies

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Primary Antibodies

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Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (AB23345)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELF5 also known as E74 Like ETS Transcription Factor 5 is a transcription factor with a mass of approximately 27 kDa. It belongs to the ETS family and plays a role in regulating gene expression by binding to specific DNA sequences. ELF5 localizes mainly in epithelial tissues where it influences development and differentiation. This targeting involves critical cellular processes particularly affecting mammary gland and placental development.
Biological function summary

ELF5 plays a significant role in maintaining the balance between cellular proliferation and differentiation. It operates within specific protein complexes to modulate the luminal epithelial cell fate in the mammary gland. ELF5 regulates critical genes responsible for alveolar development contributing to normal lactation. In placental tissues it affects trophoblast differentiation and normal placental function reflecting its impact on fetal development.

Pathways

ELF5 actively participates in pathways involving hormone response and epithelial cell differentiation. It interacts closely with proteins such as GATA3 within these pathways which help regulate luminal cell differentiation in the mammary gland. ELF5 also participates in the Wnt signaling pathway demonstrating its multifaceted roles by influencing cell fate decisions and maintaining epithelial homeostasis.

ELF5 shows connections to breast cancer and placental disorders. Changes in ELF5 expression can impact breast cancer progression by altering epithelial cell states emphasizing its predictive role in cancer biology. Aberrant ELF5 levels may contribute to conditions like pre-eclampsia through its association with critical proteins such as GATA3 affecting normal placental function and development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation.. Isoform 2 binds to DNA sequences containing the consensus nucleotide core sequence GGA[AT]. Transcriptionally activates SPRR2A and the parotid gland-specific PSP promoters.
See full target information ELF5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed36611909

2022

PTPRK, an EGFR Phosphatase, Is Decreased in CeD Biopsies and Intestinal Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Merlin Nanayakkara,Claudia Bellomo,Francesca Furone,Mariantonia Maglio,Antonella Marano,Giuliana Lania,Monia Porpora,Martina Nicoletti,Salvatore Auricchio,Maria Vittoria Barone

Frontiers in cell and developmental biology 9:647763 PubMed34055778

2021

EPC-Derived Exosomal miR-1246 and miR-1290 Regulate Phenotypic Changes of Fibroblasts to Endothelial Cells to Exert Protective Effects on Myocardial Infarction by Targeting ELF5 and SP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yulang Huang,Lifang Chen,Zongming Feng,Weixin Chen,Shaodi Yan,Rongfeng Yang,Jian Xiao,Jiajia Gao,Debao Zhang,Xiao Ke
View all publications
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Product promise

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