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AB218133

Anti-ELK1 (phospho S383) antibody

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(6 Publications)

Rabbit Polyclonal ELK1 phospho S383 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ELK1 phospho S383 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ETS domain-containing protein Elk-1, ELK1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELK1 (phospho S383) antibody (AB218133)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELK1 (phospho S383) antibody (AB218133)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human lung carcinoma tissue, labeling ELK1 (phospho S383) using ab218133 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Western blot - Anti-ELK1 (phospho S383) antibody (AB218133)
  • WB

Supplier Data

Western blot - Anti-ELK1 (phospho S383) antibody (AB218133)

All lanes:

Western blot - Anti-ELK1 (phospho S383) antibody (ab218133) at 1/300 dilution

All lanes:

mouse brain lysates

Predicted band size: 45 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ELK1 phospho S383 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P19419

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELK1 also known as ELK-1 or ETS-domain protein ELK1 is a transcription factor involved in regulating gene expression. It is a member of the ETS family of transcription factors and has a molecular mass of approximately 54 kDa. ELK1 is mainly expressed in the nucleus of cells and plays a significant role in various tissues by interacting with other proteins. It has sequence-specific DNA-binding ability which allows it to influence the transcription of target genes.
Biological function summary

ELK1 functions in the regulation of gene expression related to cellular proliferation and differentiation. It often forms a ternary complex with serum response factor (SRF) on serum response elements (SREs) to modulate the transcriptional responses of genes important for cell growth. The activation of ELK1 is phosphorylation-dependent primarily by mitogen-activated protein kinases (MAPKs) which further facilitates its role as a transcription regulator. This phosphorylation increases the transcriptional activity enabling ELK1 to actively participate in cellular responses to external signals.

Pathways

ELK1 participates prominently in the MAPK/ERK signaling pathway and the JNK signaling pathway. Through these pathways ELK1 gets activated influencing the transcription of immediate early genes responsible for cell cycle progression and apoptosis. ELK1 interacts with other proteins in these pathways such as MEK and ERK leading to a coordinated response to growth signals. These interactions underline its importance in signal transduction processes and cellular response mechanisms.

ELK1 has associations with certain cancers and neurodegenerative disorders. In particular its dysregulation has been noted in the context of breast cancer where ELK1 is implicated in abnormal cell proliferation. Similarly in neurodegenerative diseases like Alzheimer's ELK1's altered activity levels can affect neuron function and survival. Other proteins such as amyloid precursor protein in Alzheimer's or estrogen receptor in breast cancer connect with ELK1 influencing disease progression through complex signaling networks.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that binds to purine-rich DNA sequences (PubMed : 10799319, PubMed : 7889942). Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2 (PubMed : 1630903). Induces target gene transcription upon JNK and MAPK-signaling pathways stimulation (PubMed : 7889942).
See full target information ELK1 phospho S383
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 44:448-457 PubMed38767628

2024

Optimized new Shengmai powder inhibits myocardial fibrosis in heart failure by regulating the rat sarcoma/rapidly accelerated fibrosarcoma/mitogen-activated protein kinase kinase/extracellular regulated protein kinases signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhang Zeyu,Jia Zhuangzhuang,Song Yuwei,Zhang Xuan,Wang Ci,Wang Shuai,Zhang Peipei,Ren Qiuan,Wang Xianliang,Mao Jingyuan

Cell proliferation 57:e13635 PubMed38594962

2024

The antioxidant effect of tetrahedral framework nucleic acid-based delivery of small activating RNA targeting DJ-1 on retinal oxidative stress injury.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaowei Wu,Jingyi Zhu,Xianggui Zhang,Xiaoxiao Xu,Delun Luo,Yunfeng Lin,Ming Yan,Yanping Song

Marine drugs 21: PubMed37103364

2023

The Blockade of Mitogen-Activated Protein Kinase 14 Activation by Marine Natural Product Crassolide Triggers ICD in Tumor Cells and Stimulates Anti-Tumor Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Keng-Chang Tsai,Chia-Sheng Chen,Jui-Hsin Su,Yu-Ching Lee,Yu-Hwei Tseng,Wen-Chi Wei

Journal of cellular and molecular medicine 25:10724-10735 PubMed34704358

2021

MicroRNA-150 and its target ETS-domain transcription factor 1 contribute to inflammation in diabetic photoreceptors.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Yu,Michael L Ko,Gladys Y-P Ko

Communications biology 2:359 PubMed31602408

2019

Mechanical strain promotes skin fibrosis through LRG-1 induction mediated by ELK1 and ERK signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Gao,Jia Zhou,Zhibo Xie,Jing Wang,Chia-Kang Ho,Yifan Zhang,Qingfeng Li

The Journal of biological chemistry 294:6871-6887 PubMed30824540

2019

EPHB6 controls catecholamine biosynthesis by up-regulating tyrosine hydroxylase transcription in adrenal gland chromaffin cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Shi,Yujia Wang,Junzheng Peng,Shijie Qi,Nicolas Vitale,Norio Kaneda,Tomiyasu Murata,Hongyu Luo,Jiangping Wu
View all publications
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