AB34270

Anti-ELK1 (phospho S383) antibody

Name: Anti-ELK1 (phospho S383) antibody (ab34270) | Abcam
Brand: Abcam Limited
SKU: AB34270
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal ELK1 phospho S383 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human ELK1 phospho S383.

View Alternative Names

ETS domain-containing protein Elk-1, ELK1

1 Images

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Western blot - Anti-ELK1 (phospho S383) antibody (AB34270)

All lanes:

Western blot - Anti-ELK1 (phospho S383) antibody (ab34270) at 1/1000 dilution

All lanes:

recombinant Elk1 protein

Predicted band size: 45 kDa

Observed band size: 46 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

applications

Immunogen

Synthetic Peptide within Human ELK1 phospho S383. The exact immunogen used to generate this antibody is proprietary information.

P19419

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELK1 also known as ELK-1 or ETS-domain protein ELK1 is a transcription factor involved in regulating gene expression. It is a member of the ETS family of transcription factors and has a molecular mass of approximately 54 kDa. ELK1 is mainly expressed in the nucleus of cells and plays a significant role in various tissues by interacting with other proteins. It has sequence-specific DNA-binding ability which allows it to influence the transcription of target genes.

Biological function summary

ELK1 functions in the regulation of gene expression related to cellular proliferation and differentiation. It often forms a ternary complex with serum response factor (SRF) on serum response elements (SREs) to modulate the transcriptional responses of genes important for cell growth. The activation of ELK1 is phosphorylation-dependent primarily by mitogen-activated protein kinases (MAPKs) which further facilitates its role as a transcription regulator. This phosphorylation increases the transcriptional activity enabling ELK1 to actively participate in cellular responses to external signals.

Pathways

ELK1 participates prominently in the MAPK/ERK signaling pathway and the JNK signaling pathway. Through these pathways ELK1 gets activated influencing the transcription of immediate early genes responsible for cell cycle progression and apoptosis. ELK1 interacts with other proteins in these pathways such as MEK and ERK leading to a coordinated response to growth signals. These interactions underline its importance in signal transduction processes and cellular response mechanisms.

ELK1 has associations with certain cancers and neurodegenerative disorders. In particular its dysregulation has been noted in the context of breast cancer where ELK1 is implicated in abnormal cell proliferation. Similarly in neurodegenerative diseases like Alzheimer's ELK1's altered activity levels can affect neuron function and survival. Other proteins such as amyloid precursor protein in Alzheimer's or estrogen receptor in breast cancer connect with ELK1 influencing disease progression through complex signaling networks.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds to purine-rich DNA sequences (PubMed : 10799319, PubMed : 7889942). Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2 (PubMed : 1630903). Induces target gene transcription upon JNK and MAPK-signaling pathways stimulation (PubMed : 7889942).

See full target information ELK1 phospho S383

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 16:1658389 PubMed40964620

2025

Blood-spinal cord barrier alterations in a mouse model of centrifugation-induced hypergravity.

Applications

Unspecified application

Species

Unspecified reactive species

David Dubayle,Jean-Luc Morel,Sighild Lemarchant

Nature communications 11:1733 PubMed32265441

2020

Interference with ERK-dimerization at the nucleocytosolic interface targets pathological ERK1/2 signaling without cardiotoxic side-effects.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Tomasovic,Theresa Brand,Constanze Schanbacher,Sofia Kramer,Martin W Hümmert,Patricio Godoy,Wolfgang Schmidt-Heck,Peter Nordbeck,Jonas Ludwig,Susanne Homann,Armin Wiegering,Timur Shaykhutdinov,Christoph Kratz,Ruth Knüchel,Hans-Konrad Müller-Hermelink,Andreas Rosenwald,Norbert Frey,Jutta Eichler,Dobromir Dobrev,Ali El-Armouche,Jan G Hengstler,Oliver J Müller,Karsten Hinrichs,Friederike Cuello,Alma Zernecke,Kristina Lorenz

Journal of neuroinflammation 17:95 PubMed32220257

2020

Mutant Huntingtin affects toll-like receptor 4 intracellular trafficking and cytokine production in mast cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marian Jesabel Pérez-Rodríguez,Alfredo Ibarra-Sánchez,Abraham Román-Figueroa,Francisca Pérez-Severiano,Claudia González-Espinosa

FASEB journal : official publication of the Federa 33:12680-12695 PubMed31536390

2019

Insulin-like growth factor 1 signaling in tenocytes is required for adult tendon growth.

Applications

Unspecified application

Species

Unspecified reactive species

Nathaniel P Disser,Kristoffer B Sugg,Jeffrey R Talarek,Dylan C Sarver,Brennan J Rourke,Christopher L Mendias

Experimental cell research 382:111476 PubMed31255599

2019

Role of integrin-linked kinase in the hypoxia-induced phenotypic transition of pulmonary artery smooth muscle cells: Implications for hypoxic pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Jiantong Hou,Bo Liu,Boqian Zhu,Dong Wang,Yong Qiao,Erfei Luo,Abdul Qadir Nawabi,Gaoliang Yan,Chengchun Tang

View all publications

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