JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB28817

Anti-ELK1 (phospho T417) antibody

0

(1 Review)

|

(1 Publication)

Rabbit Polyclonal ELK1 phospho T417 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ELK1 phospho T417.

View Alternative Names

ETS domain-containing protein Elk-1, ELK1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELK1 (phospho T417) antibody (AB28817)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELK1 (phospho T417) antibody (AB28817)

Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left : Using Phospho-ELK-1(Thr417) Antibody (ab28817); Right : The same antibody preincubated with synthesized phosphopeptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human ELK1 phospho T417. The exact immunogen used to generate this antibody is proprietary information.

P19419

Specificity

ab28817 recognises Phospho-ELK-1(Thr417).

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity-purified from rabbit antiserum byaffinity-chromatography using epitope-specific phosphopeptide.The antibody against non-phosphopeptide was removed bychromatography using non-phosphopeptide corresponding to thephosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELK1 also known as ELK-1 or ETS-domain protein ELK1 is a transcription factor involved in regulating gene expression. It is a member of the ETS family of transcription factors and has a molecular mass of approximately 54 kDa. ELK1 is mainly expressed in the nucleus of cells and plays a significant role in various tissues by interacting with other proteins. It has sequence-specific DNA-binding ability which allows it to influence the transcription of target genes.
Biological function summary

ELK1 functions in the regulation of gene expression related to cellular proliferation and differentiation. It often forms a ternary complex with serum response factor (SRF) on serum response elements (SREs) to modulate the transcriptional responses of genes important for cell growth. The activation of ELK1 is phosphorylation-dependent primarily by mitogen-activated protein kinases (MAPKs) which further facilitates its role as a transcription regulator. This phosphorylation increases the transcriptional activity enabling ELK1 to actively participate in cellular responses to external signals.

Pathways

ELK1 participates prominently in the MAPK/ERK signaling pathway and the JNK signaling pathway. Through these pathways ELK1 gets activated influencing the transcription of immediate early genes responsible for cell cycle progression and apoptosis. ELK1 interacts with other proteins in these pathways such as MEK and ERK leading to a coordinated response to growth signals. These interactions underline its importance in signal transduction processes and cellular response mechanisms.

ELK1 has associations with certain cancers and neurodegenerative disorders. In particular its dysregulation has been noted in the context of breast cancer where ELK1 is implicated in abnormal cell proliferation. Similarly in neurodegenerative diseases like Alzheimer's ELK1's altered activity levels can affect neuron function and survival. Other proteins such as amyloid precursor protein in Alzheimer's or estrogen receptor in breast cancer connect with ELK1 influencing disease progression through complex signaling networks.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcription factor that binds to purine-rich DNA sequences (PubMed : 10799319, PubMed : 7889942). Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2 (PubMed : 1630903). Induces target gene transcription upon JNK and MAPK-signaling pathways stimulation (PubMed : 7889942).
See full target information ELK1 phospho T417
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 10:81 PubMed38360723

2024

CD24 induced cellular quiescence-like state and chemoresistance in ovarian cancer cells via miR-130a/301a-dependent CDK19 downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yeonsue Jang,Suki Kang,Hyun Ho Han,Baek Gil Kim,Nam Hoon Cho
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB34270

Anti-ELK1 (phospho S383) antibody

Western blot - Anti-ELK1 (phospho S383) antibody (AB34270)

  • 0
  • 0
Primary Antibodies

AB224929

Alexa Fluor® 488 Anti-ELK1 antibody [E277]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-ELK1 antibody [E277] (AB224929)

  • 0
  • 0
Primary Antibodies

AB227114

Anti-ELK1 antibody [E277] - BSA and Azide free

RabMAb|Recombinant|KO Validated|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELK1 antibody [E277] - BSA and Azide free (AB227114)

  • 0
  • 0
Primary Antibodies

AB188316

Anti-ELK1 antibody [EPR1913(2)]

RabMAb|Recombinant|KO Validated|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-ELK1 antibody [EPR1913(2)] (AB188316)

  • 5
  • 1
Primary Antibodies

AB32106

Anti-ELK1 antibody [E277]

RabMAb|Recombinant|KO Validated|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELK1 antibody [E277] (AB32106)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB32019

Anti-ATF2 (phospho T71) antibody [E268]

Advanced Validation|RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-ATF2 (phospho T71) antibody [E268] (AB32019)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com