Rabbit Polyclonal ELK4 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ELK4 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Balkan medical journal 40:409-4142023ELK4 Promotes Cell Cycle Progression and Stem Cell-like Characteristics in HPV-associated Cervical Cancer by Regulating the FBXO22/PTEN AxisApplications:Unspecified applicationReactive species:Unspecified reactive speciesFuxian Gao,Chunxiao Wang,Xue Bai,Jianghai Ji,Xinrui HuangPubMed 37519006
- Cell death & disease 12:9572021ELK4-mediated lncRNA SNHG22 promotes gastric cancer progression through interacting with EZH2 and regulating miR-200c-3p/Notch1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaqiong Mao,Tao Ji,Aiguo Liu,Yunqi WengPubMed 34663788
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ELK4 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P28324
Reactivity data
Select an application| WB | |
|---|---|
| Recombinant fragment - Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 1/1000 | Notes - |
Target data
Function
Involved in both transcriptional activation and repression. Interaction with SIRT7 leads to recruitment and stabilization of SIRT7 at promoters, followed by deacetylation of histone H3 at 'Lys-18' (H3K18Ac) and subsequent transcription repression. Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.
Alternative names
SAP1, ELK4, ETS domain-containing protein Elk-4, Serum response factor accessory protein 1, SAP-1, SRF accessory protein 1
Recommended products
Rabbit Polyclonal ELK4 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ELK4 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ELK4 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P28324
- Purity
- Whole antiserum
- Concentration
- Purification notes
Crude serum, final bleed
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ELK4 also known as SAP-1 (Serum Response Factor Accessory Protein 1) functions as a transcription factor within the Ets family. ELK4's molecular mass is approximately 51 kDa. This protein is expressed widely in various tissues including the brain heart and lungs. It binds to the DNA sequence GGAA/T to regulate gene expression and plays a significant role in cellular processes by modulating transcriptional response to external stimuli.
- Biological function summary
The ELK4 protein influences cell proliferation and differentiation. It forms a complex with serum response factor (SRF) to modulate the activity of immediate early genes like c-fos. The ELK4-SRF complex binds to serum response elements (SREs) within promoter regions of target genes thereby impacting cellular responses such as growth and survival. Additionally ELK4's interactions with other proteins in the nucleus enhance its function in regulating gene expression.
- Pathways
The ELK4 protein plays an important role in the MAPK/ERK signaling pathway which is important for transmitting extracellular signals to the nucleus and affecting gene expression. It interacts closely with other proteins such as ERK1/2 leading to its phosphorylation and activation. Also ELK4 participates in the Ras signaling cascade further influencing cellular responses to growth factors.
- Associated diseases and disorders
ELK4's dysfunctional regulation contributes to cancer particularly in prostate carcinoma. Aberrant ELK4 signaling affects the expression of genes related to cell proliferation and apoptosis supporting tumorigenesis. Additionally ELK4 associates with the SRF protein in the development of cardiovascular disorders where anomalous signaling can lead to pathological cardiac hypertrophy and failure.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-ELK4 antibody (ab86002)
Gel concentration: 10%
The actual band molecular weight is the result of the target protein ELK4 (47 kDa) attached to a tag (14 kDa).All lanes: Western blot - Anti-ELK4 antibody (ab86002) at 1/1000 dilution
Lane 1: uninduced culture of E. coli (plus anti thio at 1/5000 dilution)
Lane 2: induced culture of E. coli (plus anti thio at 1/5000 dilution)
SecondaryAll lanes: anti-Rabbit IgG HRP conjugated incubated for 60 minutes at 1/1000 dilution
Predicted band size: 46 kDa
Observed band size: 61 kDa
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