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AB86002

Anti-ELK4 antibody

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(4 Publications)

Rabbit Polyclonal ELK4 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ELK4 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

SAP1, ELK4, ETS domain-containing protein Elk-4, Serum response factor accessory protein 1, SAP-1, SRF accessory protein 1

1 Images
Western blot - Anti-ELK4 antibody (AB86002)
  • WB

Unknown

Western blot - Anti-ELK4 antibody (AB86002)

Gel concentration : 10%

The actual band molecular weight is the result of the target protein ELK4 (47 kDa) attached to a tag (14 kDa).

All lanes:

Western blot - Anti-ELK4 antibody (ab86002) at 1/1000 dilution

Lane 1:

uninduced culture of <I>E. coli</I> (plus anti thio at 1/5000 dilution)

Lane 2:

induced culture of <I>E. coli</I> (plus anti thio at 1/5000 dilution)

Secondary

All lanes:

anti-Rabbit IgG HRP conjugated incubated for 60 minutes at 1/1000 dilution

Predicted band size: 46 kDa

Observed band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ELK4 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P28324

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Crude serum, final bleed
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELK4 also known as SAP-1 (Serum Response Factor Accessory Protein 1) functions as a transcription factor within the Ets family. ELK4's molecular mass is approximately 51 kDa. This protein is expressed widely in various tissues including the brain heart and lungs. It binds to the DNA sequence GGAA/T to regulate gene expression and plays a significant role in cellular processes by modulating transcriptional response to external stimuli.
Biological function summary

The ELK4 protein influences cell proliferation and differentiation. It forms a complex with serum response factor (SRF) to modulate the activity of immediate early genes like c-fos. The ELK4-SRF complex binds to serum response elements (SREs) within promoter regions of target genes thereby impacting cellular responses such as growth and survival. Additionally ELK4's interactions with other proteins in the nucleus enhance its function in regulating gene expression.

Pathways

The ELK4 protein plays an important role in the MAPK/ERK signaling pathway which is important for transmitting extracellular signals to the nucleus and affecting gene expression. It interacts closely with other proteins such as ERK1/2 leading to its phosphorylation and activation. Also ELK4 participates in the Ras signaling cascade further influencing cellular responses to growth factors.

ELK4's dysfunctional regulation contributes to cancer particularly in prostate carcinoma. Aberrant ELK4 signaling affects the expression of genes related to cell proliferation and apoptosis supporting tumorigenesis. Additionally ELK4 associates with the SRF protein in the development of cardiovascular disorders where anomalous signaling can lead to pathological cardiac hypertrophy and failure.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in both transcriptional activation and repression. Interaction with SIRT7 leads to recruitment and stabilization of SIRT7 at promoters, followed by deacetylation of histone H3 at 'Lys-18' (H3K18Ac) and subsequent transcription repression. Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.
See full target information ELK4
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Balkan medical journal 40:409-414 PubMed37519006

2023

ELK4 Promotes Cell Cycle Progression and Stem Cell-like Characteristics in HPV-associated Cervical Cancer by Regulating the FBXO22/PTEN Axis

Applications

Unspecified application

Species

Unspecified reactive species

Fuxian Gao,Chunxiao Wang,Xue Bai,Jianghai Ji,Xinrui Huang

Scientific reports 13:7737 PubMed37173384

2023

FOXK1 regulates malignant progression and radiosensitivity through direct transcriptional activation of CDC25A and CDK4 in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxu Li,Juntao Lu,Lei Liu,Fei Li,Tongxin Xu,Liying Chen,Zhaoyang Yan,Yan Li,Wei Guo

Cell death & disease 12:957 PubMed34663788

2021

ELK4-mediated lncRNA SNHG22 promotes gastric cancer progression through interacting with EZH2 and regulating miR-200c-3p/Notch1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaqiong Mao,Tao Ji,Aiguo Liu,Yunqi Weng

Journal of translational medicine 19:342 PubMed34372882

2021

ELK4 promotes the development of gastric cancer by inducing M2 polarization of macrophages through regulation of the KDM5A-PJA2-KSR1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zheng,Hongmei Xu,Ya Di,Lanlan Chen,Jiao Liu,Liying Kang,Liming Gao
View all publications
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