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AB180507

Anti-ELKS antibody [EPR13777]

5

(2 Reviews)

|

(4 Publications)

Rabbit Recombinant Monoclonal ELKS antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

ELKS, KIAA1081, RAB6IP2, ERC1, ELKS/Rab6-interacting/CAST family member 1, ERC-1, Rab6-interacting protein 2

2 Images
Western blot - Anti-ELKS antibody [EPR13777] (AB180507)
  • WB

Supplier Data

Western blot - Anti-ELKS antibody [EPR13777] (AB180507)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ELKS antibody [EPR13777] (ab180507) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HEK293 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SW480 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 128 kDa

Observed band size: 130 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-ELKS antibody [EPR13777] (AB180507)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ELKS antibody [EPR13777] (AB180507)

Immunofluorescence analysis of HeLa cells labeling ELKS (red) with ab180507 at 1/250 dilution. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/250 dilution. Nuclei were counterstained with DAPI (blue).

  • Carrier free

    Anti-ELKS antibody [EPR13777] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13777

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ELKS protein also known as ERC1 or Rab6-interacting golgin is about 120-150 kDa in mass and functions as a scaffold protein. It interacts with other proteins at synaptic sites playing an important part in the regulation of neurotransmitter release. ELKS mainly locates to the nervous system but is found in other tissues as well. This protein contains coiled-coil domains facilitating binding to various partners including RIM piccolo and liprin.
Biological function summary

ELKS contributes to synaptic vesicle priming and readiness for neurotransmission. Within its role ELKS becomes part of the presynaptic active zone complex critical for proper synapse functioning. This protein scaffolds various components bringing them together for efficient synaptic signaling. By stabilizing synaptic structures ELKS ensures rapid responses to neural stimuli important for normal neuronal communication and brain function.

Pathways

ELKS proteins become integral to the synaptic vesicle cycle and neurotransmitter release pathways. ELKS interacts with proteins such as RIM and Munc13 which are essential for vesicle docking and priming. These interactions help coordinate neuronal signaling and transmitter release connecting ELKS with the broader network of synaptic activity regulators. Key pathways such as the cAMP signaling pathway depend on this fine-tuned coordination to properly translate extracellular signals into cellular responses.

ELKS shows connections with neurological conditions such as epilepsy and schizophrenia. Abnormalities in ELKS function might disrupt neural circuitry contributing to these disorders. Changes in ELKS or related proteins like piccolo or bassoon can alter synaptic balance leading to disease states. Researchers continue to investigate how modulation of ELKS and its associated proteins may offer therapeutic avenues for such conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex. May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport.
See full target information ERC1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Brain and behavior 13:e3075 PubMed37226399

2023

Effects of chronic sleep restriction on the neuro-phenotypes of Ctnnd2 knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

Man Xu,Xiaoya Wang,Luyi Wang,Shali Wang,Jing Deng,Yan Wang,Yingbo Li,Sen Pan,Ailing Liao,Yihao Tao,Shujiang Tan

The Journal of cell biology 222: PubMed36920342

2023

The exocyst complex is an essential component of the mammalian constitutive secretory pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Conceição Pereira,Danièle Stalder,Georgina S F Anderson,Amber S Shun-Shion,Jack Houghton,Robin Antrobus,Michael A Chapman,Daniel J Fazakerley,David C Gershlick

Journal of neuroinflammation 17:305 PubMed33059746

2020

Forsythoside B attenuates memory impairment and neuroinflammation via inhibition on NF-κB signaling in Alzheimer's disease.

Applications

WB

Species

Mouse

Fan'ge Kong,Xue Jiang,Ruochen Wang,Siyu Zhai,Yizhi Zhang,Di Wang

PLoS pathogens 11:e1005288 PubMed26599541

2015

The Transcription and Translation Landscapes during Human Cytomegalovirus Infection Reveal Novel Host-Pathogen Interactions.

Applications

WB

Species

Unspecified reactive species

Osnat Tirosh,Yifat Cohen,Alina Shitrit,Odem Shani,Vu Thuy Khanh Le-Trilling,Mirko Trilling,Gilgi Friedlander,Marvin Tanenbaum,Noam Stern-Ginossar
View all publications
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Product promise

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