Anti-ELKS antibody [EPR13777] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ELKS antibody [EPR13777] - BSA and Azide free (AB250209)

This data was developed using ab180507, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HeLa cells labeling ELKS (red) with ab180507 at 1/250 dilution. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/250 dilution. Nuclei were counterstained with DAPI (blue).

• WB

Lab

Western blot - Anti-ELKS antibody [EPR13777] - BSA and Azide free (AB250209)

This data was developed using ab180507, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used at 1/200000 dilution (36KDa).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 29491150)

All lanes:

Western blot - Anti-ELKS antibody [EPR13777] (<a href='/en-us/products/primary-antibodies/elks-antibody-epr13777-ab180507'>ab180507</a>) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 3:

4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 6:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 7:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Lane 8:

NR8383 (rat alveolar macrophage) whole cell lysate at 20 µg

Lane 9:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110-140 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-ELKS antibody [EPR13777] - BSA and Azide free (AB250209)

This data was developed using ab180507, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ELKS antibody [EPR13777] (<a href='/en-us/products/primary-antibodies/elks-antibody-epr13777-ab180507'>ab180507</a>) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HEK293 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SW480 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 128 kDa

Observed band size: 130 kDa

false

• WB

Lab

Western blot - Anti-ELKS antibody [EPR13777] - BSA and Azide free (AB250209)

This data was developed using ab180507, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used at 1/200000 dilution (36KDa).

Low expression : heart

All lanes:

Western blot - Anti-ELKS antibody [EPR13777] (<a href='/en-us/products/primary-antibodies/elks-antibody-epr13777-ab180507'>ab180507</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110-140 kDa

false

Exposure time: 180s