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AB264218

Anti-ELL antibody

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Rabbit Polyclonal ELL antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ELL aa 350-400.

View Alternative Names

C19orf17, ELL, RNA polymerase II elongation factor ELL, Eleven-nineteen lysine-rich leukemia protein

1 Images
Immunoprecipitation - Anti-ELL antibody (AB264218)
  • IP

Supplier Data

Immunoprecipitation - Anti-ELL antibody (AB264218)

ELL was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264218 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab264218 at 1 μg/ml.

Lane 1 : ab264218 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

All lanes:

Immunoprecipitation - Anti-ELL antibody (ab264218)

Predicted band size: 68 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human ELL aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

P55199

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mL", "IP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELL also known as Eleven-nineteen lysine-rich leukemia is a transcription elongation factor. It has a molecular mass of approximately 69 kDa. This protein facilitates the process of transcriptional elongation by RNA polymerase II. ELL is widely expressed in various tissues with notable expression in the immune and hematopoietic systems. It interacts directly with RNA polymerase II and increases the transcriptional output by suppressing pausing of the polymerase on the DNA template.
Biological function summary

ELL enhances transcription processes which are fundamental to gene expression regulation. It can form complexes with other proteins such as the ELL-associated factor (EAF) proteins which aids in its function. These complexes modulate transcriptional elongation by coordinating with transcription initiation factors and co-activators. This role is important for maintaining proper cellular functions and regulating gene expression patterns necessary for cell differentiation and proliferation.

Pathways

ELL is involved in significant cellular signaling like the transcription regulators pathway. It functions alongside other transcription regulation factors such as the p-TEFb (positive transcription elongation factor b) complex. It contributes to control transcriptional elongation cycle and maintaining proper transcriptional dynamics within cells. Through its pathway interactions ELL works with Cyclin T1 from the p-TEFb complex influencing cell cycle and development processes.

ELL has implications in leukemia especially acute myeloid leukemia (AML). Chromosomal translocations that involve ELL result in fusion proteins that disrupt normal transcription processes leading to oncogenesis. ELL fusion proteins may interact with MLL (mixed-lineage leukemia) proteins in these conditions altering regulation of target genes and contributing to leukemia pathogenesis. ELL's malfunction also connects to pathological states involving transcriptional dysregulation impacting cellular growth and differentiation pathways linked with cancerous transformations.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed : 22195968, PubMed : 23932780). Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription (PubMed : 23932780). ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation.
See full target information ELL
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