AB115027

Anti-ELL2 antibody

Name: Anti-ELL2 antibody (ab115027) | Abcam
Brand: Abcam Limited
SKU: AB115027
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal ELL2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RNA polymerase II elongation factor ELL2 aa 400-450.

View Alternative Names

RNA polymerase II elongation factor ELL2, ELL2

2 Images

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Immunoprecipitation - Anti-ELL2 antibody (AB115027)

Detection of ELL2 by Western Blot of Immunprecipitate.
ab115027, at 1 µg/ml, staining ELL2 in Hela whole cell lysate immunoprecipitated using ab115027 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ELL2 antibody (ab115027)

Predicted band size: 72 kDa

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Western blot - Anti-ELL2 antibody (AB115027)

All lanes:

Western blot - Anti-ELL2 antibody (ab115027) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 72 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RNA polymerase II elongation factor ELL2 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

O00472

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab115027 was affinity purified using an epitope specific to ELL2 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELL2 also known as Eleven Nineteen Lysine-rich Leukemia 2 is a protein involved in transcriptional regulation. This protein has a molecular mass of approximately 76 kDa. ELL2 is expressed in various tissues including both lymphoid and non-lymphoid origins. It functions as an elongation factor for RNA polymerase II modulating the rate of transcription elongation by directly interacting with the transcription machinery.

Biological function summary

ELL2 plays a significant role in RNA processing and maturation. It is a part of the ELL complex and works alongside other proteins that facilitate the transition from transcription initiation to elongation. By promoting RNA synthesis and elongation efficiency ELL2 is linked with gene expression regulation particularly within the adaptive immune system. This protein helps in optimizing the output of genes active in immune cells contributing to the functionality and differentiation of these cells.

Pathways

ELL2 participates actively in the transcription regulation pathway and its interaction with RNA polymerase II. The protein influences gene expression regulation by enhancing transcriptional elongation. ELL2 is also involved in pathways associated with the immunoglobulin production process. It interacts with other proteins such as ELL1 and pTEFb which further coordinate the elongation steps of transcription thereby impacting overall gene expression in immune-related pathways.

ELL2 associates with multiple myeloma and immunodeficiency disorders. Its dysfunction can impact immunoglobulin transcription in plasma cells potentially leading to aberrant immunoglobulin production—an element in the pathogenesis of multiple myeloma. The association of ELL2 with RNA polymerase II and its partners like pTEFb sheds light on its role in these conditions as faulty regulation of transcription elongation can lead to abnormal cell proliferation and survival characteristic of certain cancers and immune disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed : 22195968). Plays a role in immunoglobulin secretion in plasma cells : directs efficient alternative mRNA processing, influencing both proximal poly(A) site choice and exon skipping, as well as immunoglobulin heavy chain (IgH) alternative processing. Probably acts by regulating histone modifications accompanying transition from membrane-specific to secretory IgH mRNA expression.

See full target information RNA polymerase II elongation factor ELL2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Transcription 8:133-149 PubMed28340332

2017

Super elongation complex promotes early HIV transcription and its function is modulated by P-TEFb.

Applications

Unspecified application

Species

Unspecified reactive species

Alona Kuzmina,Simona Krasnopolsky,Ran Taube

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ELL2 antibody

Immunoprecipitation - Anti-ELL2 antibody (AB115027)

Western blot - Anti-ELL2 antibody (AB115027)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise