AB155775

Anti-ELMO1 antibody - C-terminal

Name: Anti-ELMO1 antibody - C-terminal (ab155775) | Abcam
Brand: Abcam Limited
SKU: AB155775

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(4 Publications)

Rabbit Polyclonal ELMO1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ELMO1 aa 650 to C-terminus.

View Alternative Names

KIAA0281, ELMO1, Engulfment and cell motility protein 1, Protein ced-12 homolog

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody - C-terminal (AB155775)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody - C-terminal (AB155775)

Immunofluorescence analysis of methanol-fixed HeLa, labeling ELMO1 with ab155775 at 1/500 dilution. Blue : Hoechst 33342 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELMO1 antibody - C-terminal (AB155775)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELMO1 antibody - C-terminal (AB155775)

Immunohistochemical analysis of paraffin-embedded Human Stomach tissue, labeling ELMO1 with ab155775 at 1/100 dilution.

Unknown

Western blot - Anti-ELMO1 antibody - C-terminal (AB155775)

12% SDS PAGE

All lanes:

Western blot - Anti-ELMO1 antibody - C-terminal (ab155775) at 1/1000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 84 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ELMO1 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92556

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 10% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELMO1 also known as Engulfment and Cell Motility 1 protein functions in the regulation of cell migration and cytoskeletal reorganization. It possesses a molecular mass of approximately 82 kDa. This protein expresses across many tissues including the brain lung and kidney indicating widespread roles in different cellular processes. Researchers frequently study ELMO1 due to its involvement in essential cellular functions linking to motility and immune responses.

Biological function summary

The protein ELMO1 participates in the regulation of Rac signaling pathways through the formation of complexes with DOCK family proteins. This complex formation contributes to the activation of Rac a small GTPase critical for actin cytoskeleton dynamics. ELMO1 affects processes such as phagocytosis and cell migration by modulating cytoskeletal configurations showing its role in the cellular machinery controlling cell shape and movement.

Pathways

The ELMO1 protein contributes to the Rac1 signaling and the phagocytosis pathway. These pathways play essential roles in integrating extracellular signals to promote cytoskeletal rearrangement and cellular movement. ELMO1 interacts with proteins like DOCK180 to facilitate these pathways. Through these interactions ELMO1 orchestrates cellular responses including actin polymerization and membrane extensions enabling effective cellular reactions to external stimuli.

Research links ELMO1 to conditions such as diabetic nephropathy and inflammatory disorders. Elevated levels of ELMO1 expression correlate with increased diabetic nephropathy progression implicating its role in this disease. In inflammatory disorders ELMO1 coordinates with proteins like Rac1 to influence macrophage function and other immune cell behaviors. These interactions show ELMO1 as an important factor in disease mechanisms where cell migration and immune responses play a significant role.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.

See full target information ELMO1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106468 PubMed37091236

2023

Latent CSN-CRL complexes are crucial for curcumin-induced apoptosis and recruited during adipogenesis to lipid droplets via small GTPase RAB18.

Applications

Unspecified application

Species

Unspecified reactive species

Dawadschargal Dubiel,Jing Wang,Roland Hartig,Supattra Chaithongyot,Wolfgang Dubiel,Michael Naumann

Cancer science 114:410-422 PubMed36310143

2022

Engulfment and cell motility protein 1 fosters reprogramming of tumor-associated macrophages in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Wen,Sheng Li,Lei Ruan,Yanping Yang,Zilin Chen,Bin Zhang,Xin Yang,Haiqing Jie,Shujuan Li,Ziwei Zeng,Sisi Liu

Frontiers in cell and developmental biology 9:723804 PubMed34993193

2021

Functional Verification of Novel Variants by Live Imaging in Zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Rongtao Xue,Ying Wang,Tienan Wang,Mei Lyu,Guiling Mo,Xijie Fan,Jianchao Li,Kuangyu Yen,Shihui Yu,Qifa Liu,Jin Xu

Molecular carcinogenesis 55:2051-2062 PubMed26756176

2016

CC chemokine ligand 18(CCL18) promotes migration and invasion of lung cancer cells by binding to Nir1 through Nir1-ELMO1/DOC180 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lihong Shi,Baogang Zhang,Xiuning Sun,Xiurong Zhang,Shijun Lv,Hongli Li,Xuejian Wang,Chunzhen Zhao,Heng Zhang,Xinpeng Xie,Ying Wang,Peng Zhang

View all publications

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