Rabbit Polyclonal ELMO1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ELMO1 aa 650 to C-terminus.
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Publications
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- Frontiers in cell and developmental biology 9:7238042021Functional Verification of Novel Variants by Live Imaging in Zebrafish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongtao Xue,Ying Wang,Tienan Wang,Mei Lyu,Guiling Mo,Xijie Fan,Jianchao Li,Kuangyu Yen,Shihui Yu,Qifa Liu,Jin XuPubMed 34993193
- Molecular carcinogenesis 55:2051-20622016CC chemokine ligand 18(CCL18) promotes migration and invasion of lung cancer cells by binding to Nir1 through Nir1-ELMO1/DOC180 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLihong Shi,Baogang Zhang,Xiuning Sun,Xiurong Zhang,Shijun Lv,Hongli Li,Xuejian Wang,Chunzhen Zhao,Heng Zhang,Xinpeng Xie,Ying Wang,Peng ZhangPubMed 26756176
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ELMO1 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q92556
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Zebrafish | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
Alternative names
KIAA0281, ELMO1, Engulfment and cell motility protein 1, Protein ced-12 homolog
Recommended products
Rabbit Polyclonal ELMO1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ELMO1 aa 650 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ELMO1 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q92556
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ELMO1 also known as Engulfment and Cell Motility 1 protein functions in the regulation of cell migration and cytoskeletal reorganization. It possesses a molecular mass of approximately 82 kDa. This protein expresses across many tissues including the brain lung and kidney indicating widespread roles in different cellular processes. Researchers frequently study ELMO1 due to its involvement in essential cellular functions linking to motility and immune responses.
- Biological function summary
The protein ELMO1 participates in the regulation of Rac signaling pathways through the formation of complexes with DOCK family proteins. This complex formation contributes to the activation of Rac a small GTPase critical for actin cytoskeleton dynamics. ELMO1 affects processes such as phagocytosis and cell migration by modulating cytoskeletal configurations showing its role in the cellular machinery controlling cell shape and movement.
- Pathways
The ELMO1 protein contributes to the Rac1 signaling and the phagocytosis pathway. These pathways play essential roles in integrating extracellular signals to promote cytoskeletal rearrangement and cellular movement. ELMO1 interacts with proteins like DOCK180 to facilitate these pathways. Through these interactions ELMO1 orchestrates cellular responses including actin polymerization and membrane extensions enabling effective cellular reactions to external stimuli.
- Associated diseases and disorders
Research links ELMO1 to conditions such as diabetic nephropathy and inflammatory disorders. Elevated levels of ELMO1 expression correlate with increased diabetic nephropathy progression implicating its role in this disease. In inflammatory disorders ELMO1 coordinates with proteins like Rac1 to influence macrophage function and other immune cell behaviors. These interactions show ELMO1 as an important factor in disease mechanisms where cell migration and immune responses play a significant role.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody - C-terminal (ab155775)
Immunofluorescence analysis of methanol-fixed HeLa, labeling ELMO1 with ab155775 at 1/500 dilution. Blue: Hoechst 33342 staining.
Western blot - Anti-ELMO1 antibody - C-terminal (ab155775)
12% SDS PAGE
All lanes: Western blot - Anti-ELMO1 antibody - C-terminal (ab155775) at 1/1000 dilution
All lanes: H1299 whole cell lysate at 30 µg
Predicted band size: 84 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELMO1 antibody - C-terminal (ab155775)
Immunohistochemical analysis of paraffin-embedded Human Stomach tissue, labeling ELMO1 with ab155775 at 1/100 dilution.
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