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AB174298

Anti-ELMO1 antibody [EPR12919]

5

(1 Review)

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(4 Publications)

Rabbit Recombinant Monoclonal ELMO1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

KIAA0281, ELMO1, Engulfment and cell motility protein 1, Protein ced-12 homolog

5 Images
Flow Cytometry (Intracellular) - Anti-ELMO1 antibody [EPR12919] (AB174298)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ELMO1 antibody [EPR12919] (AB174298)

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling ELMO1 with unpurified ab174298 at 1/200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody [EPR12919] (AB174298)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody [EPR12919] (AB174298)

Immunocytochemistry/ Immunofluorescence analysis of K562 labeling ELMO1 with ab174298 at 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 488); ab150077 at 1/1000 was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI was used to counter stain nuclei (blue).

Immunoprecipitation - Anti-ELMO1 antibody [EPR12919] (AB174298)
  • IP

Supplier Data

Immunoprecipitation - Anti-ELMO1 antibody [EPR12919] (AB174298)

Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab174298 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-ELMO1 antibody [EPR12919] (ab174298)

Predicted band size: 84 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody [EPR12919] (AB174298)
  • ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody [EPR12919] (AB174298)

LR73 cells were transfected with the indicated plasmids and stained with Alexa Fluor 488–conjugated phalloidin and anti-Elmo1 antibody.

One day after transfection, cells were washed with cold PBS, fixed in 4% paraformaldehyde for 15 min, and permeabilized with 0.1% Triton X-100 for 5 min. Next, permeabilized cells were blocked with 1% BSA for 30 min and stained with Alex Fluor 594–conjugated phalloidin and anti-Elmo1 antibody for 1 h at room temperature.

(After Figure 5 of Kim et al).

Kim, K. et al Sci Rep. 2017 Jun 30;7(1):4404. doi: 10.1038/s41598-017-04810-6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

Western blot - Anti-ELMO1 antibody [EPR12919] (AB174298)
  • WB

Supplier Data

Western blot - Anti-ELMO1 antibody [EPR12919] (AB174298)

All lanes:

Western blot - Anti-ELMO1 antibody [EPR12919] (ab174298) at 1/10000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 84 kDa

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  • HRP

    HRP Anti-ELMO1 antibody [EPR12919]

  • Carrier free

    Anti-ELMO1 antibody [EPR12919] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12919

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELMO1 also known as Engulfment and Cell Motility 1 protein functions in the regulation of cell migration and cytoskeletal reorganization. It possesses a molecular mass of approximately 82 kDa. This protein expresses across many tissues including the brain lung and kidney indicating widespread roles in different cellular processes. Researchers frequently study ELMO1 due to its involvement in essential cellular functions linking to motility and immune responses.
Biological function summary

The protein ELMO1 participates in the regulation of Rac signaling pathways through the formation of complexes with DOCK family proteins. This complex formation contributes to the activation of Rac a small GTPase critical for actin cytoskeleton dynamics. ELMO1 affects processes such as phagocytosis and cell migration by modulating cytoskeletal configurations showing its role in the cellular machinery controlling cell shape and movement.

Pathways

The ELMO1 protein contributes to the Rac1 signaling and the phagocytosis pathway. These pathways play essential roles in integrating extracellular signals to promote cytoskeletal rearrangement and cellular movement. ELMO1 interacts with proteins like DOCK180 to facilitate these pathways. Through these interactions ELMO1 orchestrates cellular responses including actin polymerization and membrane extensions enabling effective cellular reactions to external stimuli.

Research links ELMO1 to conditions such as diabetic nephropathy and inflammatory disorders. Elevated levels of ELMO1 expression correlate with increased diabetic nephropathy progression implicating its role in this disease. In inflammatory disorders ELMO1 coordinates with proteins like Rac1 to influence macrophage function and other immune cell behaviors. These interactions show ELMO1 as an important factor in disease mechanisms where cell migration and immune responses play a significant role.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
See full target information ELMO1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:475 PubMed40281518

2025

LGR6 modulates intervertebral disc degeneration through regulation of macrophage efferocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Fudong Li,Yangyang Shi,Jun Chen,Jingchuan Sun,Jiangang Shi,Kaiqiang Sun,Bing Zheng

International journal of molecular sciences 24: PubMed37175809

2023

ELMO1 Deficiency Reduces Neutrophil Chemotaxis in Murine Peritonitis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxiang Yu,Xiaoke Geng,Huibing Liu,Yunyun Zhang,Xiumei Cao,Baojie Li,Jianshe Yan

Oncology letters 16:3481-3488 PubMed30127952

2018

Downregulation of Dock1 and Elmo1 suppresses the migration and invasion of triple-negative breast cancer epithelial cells through the RhoA/Rac1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yueyang Liang,Shushu Wang,Yi Zhang

Scientific reports 7:4404 PubMed28667327

2017

Intermolecular steric inhibition of Ephexin4 is relieved by Elmo1.

Applications

Unspecified application

Species

Unspecified reactive species

Kwanhyeong Kim,Juyeon Lee,Sang-Ah Lee,Hyunji Moon,Boyeon Park,Deokhwan Kim,Young-Eun Joo,Daeho Park
View all publications
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Product promise

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