Anti-ELMO1 antibody [EPR12919] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ELMO1 antibody [EPR12919] - BSA and Azide free (AB222227)

This Flow Cyt data was generated using the same atni-ELMO1 antibody clone,EPR12919, in a different buffer formulation (cat

ab174298).

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling ELMO1 with unpurified ab174298 at 1/200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody [EPR12919] - BSA and Azide free (AB222227)

Immunocytochemistry/ Immunofluorescence analysis of K562 labeling ELMO1 with ab222227 at 1/250 dilution. Goat anti rabbit IgG(Alexa Fluor^® 488); ab150077 at 1/1000 was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI was used to counter stain nuclei (blue).

• IP

Supplier Data

Immunoprecipitation - Anti-ELMO1 antibody [EPR12919] - BSA and Azide free (AB222227)

This IP data was generated using the same atni-ELMO1 antibody clone, EPR12919, in a different buffer formulation (cat# ab174298).

Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab174298 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-ELMO1 antibody [EPR12919] (<a href='/en-us/products/primary-antibodies/elmo1-antibody-epr12919-ab174298'>ab174298</a>)

Predicted band size: 84 kDa

false

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody [EPR12919] - BSA and Azide free (AB222227)

LR73 cells were transfected with the indicated plasmids and stained with Alexa Fluor 488–conjugated phalloidin and anti-Elmo1 antibody.

One day after transfection, cells were washed with cold PBS, fixed in 4% paraformaldehyde for 15 min, and permeabilized with 0.1% Triton X-100 for 5 min. Next, permeabilized cells were blocked with 1% BSA for 30 min and stained with Alex Fluor 594–conjugated phalloidin and anti-Elmo1 antibody for 1 h at room temperature.

(After Figure 5 of Kim et al).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174298).

Kim, K. et al Sci Rep. 2017 Jun 30;7(1):4404. doi: 10.1038/s41598-017-04810-6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.