AB181234

Anti-ELMO1 + ELMO2 antibody [EPR13567]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal ELMO2 antibody. Suitable for WB and reacts with Human, Recombinant fragment - Human samples. Cited in 3 publications.

View Alternative Names

CED12A, KIAA1834, ELMO2, Engulfment and cell motility protein 2, Protein ced-12 homolog A, hCed-12A, KIAA0281, ELMO1, Engulfment and cell motility protein 1, Protein ced-12 homolog

2 Images

Supplier Data

Western blot - Anti-ELMO1 + ELMO2 antibody [EPR13567] (AB181234)

All lanes:

Western blot - Anti-ELMO1 + ELMO2 antibody [EPR13567] (ab181234) at 1/2000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 83 kDa

false

Supplier Data

Western blot - Anti-ELMO1 + ELMO2 antibody [EPR13567] (AB181234)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ELMO1 + ELMO2 antibody [EPR13567] (ab181234) at 1/2000 dilution

Lane 1:

ELMO2 Human Recombinant Protein

Lane 2:

ELMO1 Human Recombinant Protein

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 83 kDa

false

Exposure time: 20s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13567

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELMO1 and ELMO2 are proteins that play a role in intracellular signaling and cytoskeletal organization. ELMO1 is approximately 82 kDa in mass while ELMO2 is slightly smaller at around 80 kDa. These proteins are expressed in various tissues including the brain heart and lymphoid tissues. ELMO1 and ELMO2 interact with Dock180 a guanine nucleotide exchange factor to modulate cell morphology and migration by controlling actin cytoskeleton dynamics.

Biological function summary

ELMO1 and ELMO2 facilitate the regulation of cell migration phagocytosis and apoptosis. They are parts of a larger protein complex involving Dock180 and Rac1 contributing to the reorganization of the actin cytoskeleton. The ELMO-Dock180 complex acts as a critical mediator for the Rac signaling pathway affecting how cells respond to external signals that guide movement and cell survival.

Pathways

ELMO1 and ELMO2 participate in signaling pathways like the Rac1 pathway and the PI3K/Akt pathway. Through interactions with Rac1 these proteins regulate cytoskeletal rearrangements essential for various cellular processes including movement and immune cell function. ELMO1 and ELMO2 also connect with PI3K influencing the PI3K/Akt signaling cascade that is involved in cell growth and survival.

ELMO1 and ELMO2 have implications in cancer and autoimmune diseases. Studies link ELMO1 to increased susceptibility to systemic lupus erythematosus. Altered expression of ELMO2 is associated with cancer progression highlighting its potential as a therapeutic target. In cancer the interaction between ELMO proteins and Rac1 can enhance cell migration contributing to metastasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.

See full target information ELMO2

Additional targets

ELMO1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancers 14: PubMed36551595

2022

Proteome-Wide Identification of RNA-Dependent Proteins in Lung Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Varshni Rajagopal,Astrid-Solveig Loubal,Niklas Engel,Elsa Wassmer,Jeanette Seiler,Oliver Schilling,Maiwen Caudron-Herger,Sven Diederichs

Biochimica et biophysica acta 1863:2892-2904 PubMed27627840

2016

Integrin-linked kinase and ELMO2 modulate recycling endosomes in keratinocytes.

Applications

Unspecified application

Species

Mouse

Ernest Ho,Iordanka A Ivanova,Lina Dagnino

Molecular biology of the cell 26:2712-25 PubMed25995380

2015

An ELMO2-RhoG-ILK network modulates microtubule dynamics.

Applications

Unspecified application

Species

Mouse

Bradley C Jackson,Iordanka A Ivanova,Lina Dagnino

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ELMO1 + ELMO2 antibody [EPR13567]

Western blot - Anti-ELMO1 + ELMO2 antibody [EPR13567] (AB181234)

Western blot - Anti-ELMO1 + ELMO2 antibody [EPR13567] (AB181234)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (3)

Product promise