Goat Polyclonal ELMO2 antibody. Suitable for Flow Cyt, IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ELMO2 aa 700 to C-terminus.
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Publications
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- PeerJ 8:e89102020ELMO2 association with Gi2 regulates pancreatic cancer cell chemotaxis and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYecheng Wang et. al.PubMed 32292657
- Developmental cell 51:460-475.e102019Membrane Tension Orchestrates Rear Retraction in Matrix-Directed Cell Migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph H R Hetmanski et. al.PubMed 31607653
- The Journal of cell biology 207:577-872014An Elmo-Dock complex locally controls Rho GTPases and actin remodeling during cadherin-mediated adhesion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher P Toret et. al.PubMed 25452388
- The Journal of cell biology 204:265-792014A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell-cell adhesion.Applications:WB, ICC/IFReactive species:Drosophila melanogasterChristopher P Toret,Michael V D'Ambrosio,Ronald D Vale,Michael A Simon,W James NelsonPubMed 24446484
- Experimental cell research 317:1701-132011Ephexin4 and EphA2 mediate resistance to anoikis through RhoG and phosphatidylinositol 3-kinase.
- The Journal of cell biology 190:461-772010Ephexin4 and EphA2 mediate cell migration through a RhoG-dependent mechanism.
- Journal of leukocyte biology 80:953-602006Signal transduction induced by apoptotic cells inhibits HIV transcription in monocytes/macrophages.Applications:WBReactive species:Unspecified reactive speciesBethsebah N Gekonge et. al.PubMed 16885500
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.05% Tris- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ELMO2 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96JJ3
Reactivity data
Select an application| Flow Cyt | IHC-P | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected |
| Rat | Expected | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3.00000-6.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Rat | Dilution info 1.00000-3.00000 µg/mL | Notes - |
Species Mouse | Dilution info 1.00000-3.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
Alternative names
CED12A, KIAA1834, ELMO2, Engulfment and cell motility protein 2, Protein ced-12 homolog A, hCed-12A
Recommended products
Goat Polyclonal ELMO2 antibody. Suitable for Flow Cyt, IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ELMO2 aa 700 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.05% Tris- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ELMO2 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96JJ3
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ELMO2 also known as Engulfment and Cell Motility Protein 2 mechanically functions as an important regulator of cell migration and phagocytosis. It weighs approximately 82 kDa. This protein interacts extensively with the cytoplasmic tail of transmembrane receptors aiding in intracellular signaling cascades. ELMO2 is expressed in various tissues with notable levels in the brain kidney and spleen indicating its wide biological roles.
- Biological function summary
ELMO2 plays a significant role in the regulation of the actin cytoskeleton which is important in cell motility and shape. It forms part of the ELMO/EPS8/SOS1 complex where it facilitates actin remodeling and cell surface receptor signaling. The protein acts as a guanine nucleotide exchange factor (GEF) guiding the activation of small GTPases like Rac1 essential for cytoskeletal reorganization and cellular responses to external stimuli.
- Pathways
ELMO2 engages importantly in the PI3K/AKT signaling pathway and the phagocytosis regulatory pathway. It collaborates with the proteins such as DOCK180 functioning together in the phagocytosis of apoptotic cells. This association ensures the efficient clearance of dying cells maintaining tissue homeostasis and immune system balance.
- Associated diseases and disorders
ELMO2 connects directly to several cancer types and neurodegenerative diseases. Its dysregulation is often observed in certain cancers such as breast cancer where it influences metastasis via alterations in cell migration. ELMO2 is also linked to Alzheimer's disease interacting with proteins like amyloid precursor protein (APP) suggesting a role in the pathological changes seen in neuronal cell environments.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
Western blot - Anti-ELMO2 antibody (ab2240)
Detected by chemiluminescence.
All lanes: Western blot - Anti-ELMO2 antibody (ab2240) at 0.5 µg
Lane 1: Jurkat cell lysate at 35 µg/mL
Lane 2: HEK293 cell lysate at 35 µg/mL
Lane 3: RAW264.7 cell lysate at 35 µg/mL
Lane 4: KNRK cell lysate at 35 µg/mL
Immunocytochemistry/ Immunofluorescence - Anti-ELMO2 antibody (ab2240)
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunocytochemistry/ Immunofluorescence - Anti-ELMO2 antibody (ab2240)
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
Western blot - Anti-ELMO2 antibody (ab2240)
All lanes: Western blot - Anti-ELMO2 antibody (ab2240) at 0.5 µg/mL
Lane 2: Human cerebellum tissue lysate at 35 µg
Lane 2: Rat brain tissue lysate at 35 µg
Flow Cytometry - Anti-ELMO2 antibody (ab2240)
Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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