AB2240

Anti-ELMO2 antibody

Name: Anti-ELMO2 antibody (ab2240) | Abcam
Brand: Abcam Limited
SKU: AB2240
Price: 520 USD
Availability: InStock

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(7 Publications)

Goat Polyclonal ELMO2 antibody. Suitable for Flow Cyt, IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ELMO2 aa 700 to C-terminus.

View Alternative Names

CED12A, KIAA1834, ELMO2, Engulfment and cell motility protein 2, Protein ced-12 homolog A, hCed-12A

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-ELMO2 antibody (AB2240)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ELMO2 antibody (AB2240)

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ELMO2 antibody (AB2240)

Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).

Supplier Data

Western blot - Anti-ELMO2 antibody (AB2240)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-ELMO2 antibody (ab2240) at 0.5 µg

Lane 1:

Jurkat cell lysate at 35 µg/mL

Lane 2:

HEK293 cell lysate at 35 µg/mL

Lane 3:

RAW264.7 cell lysate at 35 µg/mL

Lane 4:

KNRK cell lysate at 35 µg/mL

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Supplier Data

Western blot - Anti-ELMO2 antibody (AB2240)

All lanes:

Western blot - Anti-ELMO2 antibody (ab2240) at 0.5 µg/mL

Lane 2:

Human cerebellum tissue lysate at 35 µg

Lane 2:

Rat brain tissue lysate at 35 µg

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Flow Cytometry - Anti-ELMO2 antibody (AB2240)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-ELMO2 antibody (AB2240)

Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Flow Cyt, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ELMO2 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96JJ3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3-6 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.05% Tris

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELMO2 also known as Engulfment and Cell Motility Protein 2 mechanically functions as an important regulator of cell migration and phagocytosis. It weighs approximately 82 kDa. This protein interacts extensively with the cytoplasmic tail of transmembrane receptors aiding in intracellular signaling cascades. ELMO2 is expressed in various tissues with notable levels in the brain kidney and spleen indicating its wide biological roles.

Biological function summary

ELMO2 plays a significant role in the regulation of the actin cytoskeleton which is important in cell motility and shape. It forms part of the ELMO/EPS8/SOS1 complex where it facilitates actin remodeling and cell surface receptor signaling. The protein acts as a guanine nucleotide exchange factor (GEF) guiding the activation of small GTPases like Rac1 essential for cytoskeletal reorganization and cellular responses to external stimuli.

Pathways

ELMO2 engages importantly in the PI3K/AKT signaling pathway and the phagocytosis regulatory pathway. It collaborates with the proteins such as DOCK180 functioning together in the phagocytosis of apoptotic cells. This association ensures the efficient clearance of dying cells maintaining tissue homeostasis and immune system balance.

ELMO2 connects directly to several cancer types and neurodegenerative diseases. Its dysregulation is often observed in certain cancers such as breast cancer where it influences metastasis via alterations in cell migration. ELMO2 is also linked to Alzheimer's disease interacting with proteins like amyloid precursor protein (APP) suggesting a role in the pathological changes seen in neuronal cell environments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.

See full target information ELMO2

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

PeerJ 8:e8910 PubMed32292657

2020

ELMO2 association with Gi2 regulates pancreatic cancer cell chemotaxis and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yecheng Wang,Hongyan Li,Fei Li

Developmental cell 51:460-475.e10 PubMed31607653

2019

Membrane Tension Orchestrates Rear Retraction in Matrix-Directed Cell Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph H R Hetmanski,Henry de Belly,Ignacio Busnelli,Thomas Waring,Roshna V Nair,Vanesa Sokleva,Oana Dobre,Angus Cameron,Nils Gauthier,Christophe Lamaze,Joe Swift,Aránzazu Del Campo,Tobias Starborg,Tobias Zech,Jacky G Goetz,Ewa K Paluch,Jean-Marc Schwartz,Patrick T Caswell

The Journal of cell biology 207:577-87 PubMed25452388

2014

An Elmo-Dock complex locally controls Rho GTPases and actin remodeling during cadherin-mediated adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher P Toret,Caitlin Collins,W James Nelson

The Journal of cell biology 204:265-79 PubMed24446484

2014

A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell-cell adhesion.

Applications

WB, ICC/IF

Species

Drosophila melanogaster, Drosophila melanogaster

Christopher P Toret,Michael V D'Ambrosio,Ronald D Vale,Michael A Simon,W James Nelson

Experimental cell research 317:1701-13 PubMed21621533

2011

Ephexin4 and EphA2 mediate resistance to anoikis through RhoG and phosphatidylinositol 3-kinase.

Applications

Species

Human

Kohei Harada,Nao Hiramoto-Yamaki,Manabu Negishi,Hironori Katoh

The Journal of cell biology 190:461-77 PubMed20679435

2010

Ephexin4 and EphA2 mediate cell migration through a RhoG-dependent mechanism.

Applications

Species

Human

Nao Hiramoto-Yamaki,Shingo Takeuchi,Shuhei Ueda,Kohei Harada,Satoshi Fujimoto,Manabu Negishi,Hironori Katoh

Journal of leukocyte biology 80:953-60 PubMed16885500

2006

Signal transduction induced by apoptotic cells inhibits HIV transcription in monocytes/macrophages.

Applications

Species

Unspecified reactive species

Bethsebah N Gekonge,Gillian Schiralli,Robert A Schlegel,Andrew J Henderson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ELMO2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ELMO2 antibody (AB2240)

Immunocytochemistry/ Immunofluorescence - Anti-ELMO2 antibody (AB2240)

Western blot - Anti-ELMO2 antibody (AB2240)

Western blot - Anti-ELMO2 antibody (AB2240)

Flow Cytometry - Anti-ELMO2 antibody (AB2240)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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