Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (AB248088)

This data was developed using ab124994, the same antibody clone in a different buffer formulation.

ab124994 at 1/100 dilution staining Elongation factor 1-gamma in HeLa cells by Immunofluorescence.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (AB248088)

This data was developed using ab124994, the same antibody clone in a different buffer formulation.

ab124994 at 1/250 dilution staining Elongation factor 1-gamma in paraffin-embedded Human pancreatic adenocarcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (AB248088)

This data was developed using ab124994, the same antibody clone in a different buffer formulation.

Overlay histogram showing HeLa cells stained with ab124994 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124994, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

• WB

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Western blot - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (AB248088)

This data was developed using ab124994, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Elongation factor 1-gamma antibody [EPR7200] (<a href='/en-us/products/primary-antibodies/elongation-factor-1-gamma-antibody-epr7200-ab124994'>ab124994</a>) at 1/10000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HL-60 cell lysate at 10 µg

Lane 3:

C6 cell lysate at 10 µg

Lane 4:

RAW 264.7 cell lysate at 10 µg

Lane 5:

PC-12 cell lysate at 10 µg

Lane 6:

NIH/3T3 cell lysate at 10 µg

Lane 7:

HepG2 cell lysate at 10 µg

Lane 8:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP-conjugated at 1/2000 dilution

Predicted band size: 50 kDa

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• OI-RD Scanning

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OI-RD Scanning - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (AB248088)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.