Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863)

Rabbit Recombinant Monoclonal Elongin-C antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Drosophila melanogaster, Cat, Dog, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ELOC conjugated to Keyhole Limpet Haemocyanin.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (AB313863), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Human ELOC conjugated to Keyhole Limpet Haemocyanin. Database link Q15369

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P
Human	Tested	Expected
Mouse	Expected	Tested
Cat	Tested	Expected
Dog	Tested	Expected
Drosophila melanogaster	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/5000 - 1/50000	Notes -
Species Drosophila melanogaster	Dilution info 1/5000 - 1/50000	Notes -
Species Cat	Dilution info 1/5000 - 1/50000	Notes -
Species Dog	Dilution info 1/5000 - 1/50000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Drosophila melanogaster, Cat, Dog	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information ELOC

Function

SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex) (PubMed:7821821). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells (By similarity). Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins (PubMed:10205047, PubMed:12004076, PubMed:12050673, PubMed:15590694, PubMed:21199876, PubMed:26138980, PubMed:29775578, PubMed:29779948, PubMed:30166453, PubMed:33268465, PubMed:38326650). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes (PubMed:10205047, PubMed:12004076, PubMed:12050673, PubMed:15590694). Component the von Hippel-Lindau ubiquitination complex CBC(VHL) (PubMed:10205047, PubMed:12004076, PubMed:12050673, PubMed:15590694). A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed:26138980, PubMed:29775578, PubMed:29779948). The ECS(ASB9) complex mediates ubiquitination and degradation of CKB (PubMed:33268465). As part of a multisubunit ubiquitin ligase complex, polyubiquitinates monoubiquitinated POLR2A (PubMed:19920177). ECS(LRR1) ubiquitinates MCM7 and promotes CMG replisome disassembly by VCP and chromatin extraction during S-phase (By similarity). (Microbial infection) Following infection by HIV-1 virus, component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) hijacked by the HIV-1 Vif protein, which catalyzes ubiquitination and degradation of APOBEC3F and APOBEC3G (PubMed:18562529, PubMed:20532212, PubMed:22190037, PubMed:24225024, PubMed:24402281, PubMed:36754086). The complex can also ubiquitinate APOBEC3H to some extent (PubMed:37640699).

Alternative names

TCEB1, ELOC, Elongin-C, EloC, Elongin 15 kDa subunit, RNA polymerase II transcription factor SIII subunit C, SIII p15, Transcription elongation factor B polypeptide 1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Human ELOC conjugated to Keyhole Limpet Haemocyanin. Database link Q15369
Carrier free: Yes
Clone number: HL1683
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Aliquoting information: Upon delivery aliquot

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Elongin C also known as Elongin BC is a small protein with a mass of approximately 11 kDa. This protein is part of the Elongin complex which includes Elongin B and Elongin A. It is widely expressed in various tissues facilitating its role in transcriptional regulation. Elongin C works mechanically by forming a complex with Elongin B to elongate RNA polymerase II transcription and it acts as an adaptor in various cellular processes.

Biological function summary

Elongin C participates in stabilizing the association of Elongin A with RNA polymerase II. It is an essential component of the transcription elongation complex influencing the transcriptional activity of genes. By forming a complex with Elongin B it propels the transcription process forward particularly in response to signals that regulate transcription elongation. Elongin C also connects with the cullin-RING E3 ubiquitin ligase (CRL) complex contributing to protein degradation pathways.

Pathways

Elongin C plays a significant role in the ubiquitin-proteasome system which targets proteins for degradation. It functions alongside Elongin A and Elongin B in this pathway where these components coordinate to control protein levels in the cell. Elongin C associates with the VHL (Von Hippel-Lindau) tumor suppressor protein in the pathway responsible for hypoxia-inducible factor (HIF) degradation therefore influencing cellular responses to oxygen availability.

Associated diseases and disorders

Alterations in Elongin C are implicated in cancer and von Hippel-Lindau disease. The disruption of its function can affect the stability and degradation of HIF leading to aberrant cellular proliferation and tumor progression. Elongin C through interaction with VHL highlights its importance in managing proper cell growth and differentiation underpinning its potential role in developing therapeutic targets for cancer treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863)
Paraffin-embedded mouse muscle tissue stained for TCEB1 protein at cytoplasm with ab313863 at a 1/100 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863)
Samples were seperated by 15% SDS-PAGE.
All lanes: Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863) at 1/50000 dilution
Lane 1: Jurkat whole cell extracts at 30 µg
Lane 2: Raji whole cell extracts at 30 µg
Lane 3: NCI-H929 whole cell extracts at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 12 kDa
Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863)
All lanes: Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863) at 1/50000 dilution
Lane 1: MDCK whole cell extract at 30 µg
Lane 2: PG-4 whole cell extract at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 12 kDa
Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863)
All lanes: Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863) at 1/500 dilution
Lane 1: Non-transfected 293T whole cell extract at 30 µg
Lane 2: Transfected 293T whole cell extract with Tcb1 shRNA at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 12 kDa
Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863)
All lanes: Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863) at 1/1000 dilution
All lanes: Whole S2 cell extract at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 12 kDa
Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863)
All lanes: Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863) at 1/50000 dilution
Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell extract at 30 µg
Lane 2: Raji (Human Burkitt's lymphoma cell line) whole cell extract at 30 µg
Lane 3: NCI-H929 (Human bone marrow lymphoblast cell line) whole cell extract at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 12 kDa
Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863)
All lanes: Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863) at 1/5000 dilution
Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: A431 (Human epidermoid carcinoma cell line) whole cell extract at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG antibody

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Visit the general protocols
Visit troubleshooting

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Elongin-C antibody [HL1683] - BSA and Azide free

Images

Key facts

Immunogen

Reactivity data

WB

TestedTested

ExpectedExpected

IHC-P

TestedTested

ExpectedExpected

Target data

Function

Alternative names

Recommended products

Key facts

Storage

Notes

Supplementary info

Product promise

Tested

Expected

Predicted

Not recommended

7 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863)

Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863)

Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863)

Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863)

Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863)

Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863)

Western blot - Anti-Elongin-C antibody [HL1683] - BSA and Azide free (ab313863)

Downloads

Product protocols

Tested
Tested

Expected
Expected

Tested
Tested

Expected
Expected