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Rabbit Polyclonal ELOVL4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ELOVL4 aa 250 to C-terminus.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human ELOVL4 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9GZR5
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested
Rat
Tested
Expected
Expected
Cynomolgus monkey
Predicted
Predicted
Predicted
Rhesus monkey
Predicted
Predicted
Predicted

Tested
Tested

Species

Rat

Dilution info

1/500.00000 - 1/5000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/5000.00000

Notes

-

Predicted
Predicted

Species

Cynomolgus monkey, Rhesus monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/200.00000

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cynomolgus monkey, Rhesus monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/200.00000

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cynomolgus monkey, Rhesus monkey

Dilution info

-

Notes

-

Target data

Function

Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that catalyzes the synthesis of very long chain saturated (VLC-SFA) and polyunsaturated (PUFA) fatty acids that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May play a critical role in early brain and skin development.

Alternative names

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Rabbit Polyclonal ELOVL4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ELOVL4 aa 250 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human ELOVL4 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9GZR5
Purification technique

Affinity purification Protein G

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

This enzyme contributes to the production of essential lipids that maintain cellular structure and function. ELOVL4 integrates into larger protein complexes that regulate its activity or stabilize its function. It influences the quality of long-chain ceramides and other sphingolipids which impact barrier functions particularly in the skin and retina. Moreover it participates in maintaining membrane integrity and dynamics where these specialized lipids concentrate.

Activity summary

ELOVL4 or Elongation of Very Long Chain Fatty Acids Protein 4 plays a critical role in the biosynthesis of very long chain fatty acids. It weighs approximately 32 kDa. It elongates saturated and polyunsaturated fatty acids extending carbon chains to lengths exceeding 24 carbons. ELOVL4 is found mainly in the retina skin and brain reflecting its significance in these tissues. Its mechanical function involves adding two carbons per cycle to endogenous and dietary fatty acids substrates.

Pathways

ELOVL4 participates prominently in sphingolipid and lipid metabolism pathways. It interacts with other elongases and desaturases such as ELOVL1 to execute its elongation tasks. This protein's activity bears importance in both the synthesis and modification phases of very long-chain fatty acids critical to cellular processes. The sphingolipid pathway necessitates ELOVL4 in producing functional lipids ensuring proper membrane properties and signaling events.

Associated diseases and disorders

Mutations in the ELOVL4 gene have links to conditions like Stargardt-like macular dystrophy and ichthyosis. Stargardt disease involves retinal degeneration due to disruptions in photoreceptor cell function attributed to defective fatty acid elongation. In skin-related disorders like ichthyosis ELOVL4 mutations disturb lipid processing affecting skin barrier formation. Pathologically its dysfunction associates with other lipogenic and barrier-forming proteins including ceramide synthases impacting the synthesis of necessary lipid components.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ELOVL4 antibody (ab224608), expandable thumbnail

    Western blot - Anti-ELOVL4 antibody (ab224608)

    Blocking: 5% milk (non-fat dry milk dissolved in PBST) incubate overnight under 4 oC.
    Primary antibody incubation: Dilute primary antibody with 5% milk (non-fat dry milk dissolved in PBST), incubate overnight at 4oC.
    Washing: PBST, 4 times, 10 minutes.
    Secondary antibody incubation: Dilute secondary antibody at 1/10000 or 1/20000 with 5% milk (non-fat dry milk dissolved in PBST), incubate for 1 hour under 25oC.
    Washing: PBST, 4 times, 10 minutes.

    All lanes: Western blot - Anti-ELOVL4 antibody (AB224608) at 1/500 dilution

    Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

    Lane 2: Rat heart lysate

    Lane 3: Rat liver lysate

    Secondary

    All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 37 kDa

    Observed band size: 37 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL4 antibody (ab224608), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL4 antibody (ab224608)

    Paraffin-embedded human testis tissue stained for ELOVL4 using ab224608 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL4 antibody (ab224608), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL4 antibody (ab224608)

    Paraffin-embedded human small intestine tissue stained for ELOVL4 using ab224608 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-ELOVL4 antibody (ab224608), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ELOVL4 antibody (ab224608)

    HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ELOVL4 (green) using ab224608 at 1/100 dilution in ICC/IF.

    Secondary antibody: Alexa Fluor® 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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