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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Polyclonal ELOVL4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ELOVL4 aa 250 to C-terminus.
IgG
Rabbit
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Rat | Tested | Expected | Expected |
Cynomolgus monkey | Predicted | Predicted | Predicted |
Rhesus monkey | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cynomolgus monkey, Rhesus monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cynomolgus monkey, Rhesus monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cynomolgus monkey, Rhesus monkey | Dilution info - | Notes - |
Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that catalyzes the synthesis of very long chain saturated (VLC-SFA) and polyunsaturated (PUFA) fatty acids that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May play a critical role in early brain and skin development.
Elongation of very long chain fatty acids protein 4, 3-keto acyl-CoA synthase ELOVL4, ELOVL fatty acid elongase 4, Very long chain 3-ketoacyl-CoA synthase 4, Very long chain 3-oxoacyl-CoA synthase 4, ELOVL FA elongase 4, ELOVL4
Rabbit Polyclonal ELOVL4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ELOVL4 aa 250 to C-terminus.
Elongation of very long chain fatty acids protein 4, 3-keto acyl-CoA synthase ELOVL4, ELOVL fatty acid elongase 4, Very long chain 3-ketoacyl-CoA synthase 4, Very long chain 3-oxoacyl-CoA synthase 4, ELOVL FA elongase 4, ELOVL4
IgG
Rabbit
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Protein G
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This enzyme contributes to the production of essential lipids that maintain cellular structure and function. ELOVL4 integrates into larger protein complexes that regulate its activity or stabilize its function. It influences the quality of long-chain ceramides and other sphingolipids which impact barrier functions particularly in the skin and retina. Moreover it participates in maintaining membrane integrity and dynamics where these specialized lipids concentrate.
ELOVL4 or Elongation of Very Long Chain Fatty Acids Protein 4 plays a critical role in the biosynthesis of very long chain fatty acids. It weighs approximately 32 kDa. It elongates saturated and polyunsaturated fatty acids extending carbon chains to lengths exceeding 24 carbons. ELOVL4 is found mainly in the retina skin and brain reflecting its significance in these tissues. Its mechanical function involves adding two carbons per cycle to endogenous and dietary fatty acids substrates.
ELOVL4 participates prominently in sphingolipid and lipid metabolism pathways. It interacts with other elongases and desaturases such as ELOVL1 to execute its elongation tasks. This protein's activity bears importance in both the synthesis and modification phases of very long-chain fatty acids critical to cellular processes. The sphingolipid pathway necessitates ELOVL4 in producing functional lipids ensuring proper membrane properties and signaling events.
Mutations in the ELOVL4 gene have links to conditions like Stargardt-like macular dystrophy and ichthyosis. Stargardt disease involves retinal degeneration due to disruptions in photoreceptor cell function attributed to defective fatty acid elongation. In skin-related disorders like ichthyosis ELOVL4 mutations disturb lipid processing affecting skin barrier formation. Pathologically its dysfunction associates with other lipogenic and barrier-forming proteins including ceramide synthases impacting the synthesis of necessary lipid components.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking: 5% milk (non-fat dry milk dissolved in PBST) incubate overnight under 4 oC.
Primary antibody incubation: Dilute primary antibody with 5% milk (non-fat dry milk dissolved in PBST), incubate overnight at 4oC.
Washing: PBST, 4 times, 10 minutes.
Secondary antibody incubation: Dilute secondary antibody at 1/10000 or 1/20000 with 5% milk (non-fat dry milk dissolved in PBST), incubate for 1 hour under 25oC.
Washing: PBST, 4 times, 10 minutes.
All lanes: Western blot - Anti-ELOVL4 antibody (AB224608) at 1/500 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2: Rat heart lysate
Lane 3: Rat liver lysate
All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Paraffin-embedded human testis tissue stained for ELOVL4 using ab224608 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for ELOVL4 using ab224608 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ELOVL4 (green) using ab224608 at 1/100 dilution in ICC/IF.
Secondary antibody: Alexa Fluor® 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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