AB224608

Anti-ELOVL4 antibody

Name: Anti-ELOVL4 antibody (ab224608) | Abcam
Brand: Abcam Limited
SKU: AB224608

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(2 Publications)

Rabbit Polyclonal ELOVL4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Elongation of very long chain fatty acids protein 4 aa 250 to C-terminus.

View Alternative Names

Very long chain fatty acid elongase 4, 3-keto acyl-CoA synthase ELOVL4, ELOVL fatty acid elongase 4, Elongation of very long chain fatty acids protein 4, Very long chain 3-ketoacyl-CoA synthase 4, Very long chain 3-oxoacyl-CoA synthase 4, ELOVL FA elongase 4, ELOVL4

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL4 antibody (AB224608)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL4 antibody (AB224608)

Paraffin-embedded human small intestine tissue stained for ELOVL4 using ab224608 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL4 antibody (AB224608)

Paraffin-embedded human testis tissue stained for ELOVL4 using ab224608 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ELOVL4 antibody (AB224608)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ELOVL4 antibody (AB224608)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ELOVL4 (green) using ab224608 at 1/100 dilution in ICC/IF.

Secondary antibody : Alexa Fluor^® 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supplier Data

Western blot - Anti-ELOVL4 antibody (AB224608)

Blocking : 5% milk (non-fat dry milk dissolved in PBST) incubate overnight under 4 ^oC.
Primary antibody incubation : Dilute primary antibody with 5% milk (non-fat dry milk dissolved in PBST), incubate overnight at 4^oC.
Washing : PBST, 4 times, 10 minutes.
Secondary antibody incubation : Dilute secondary antibody at 1/10000 or 1/20000 with 5% milk (non-fat dry milk dissolved in PBST), incubate for 1 hour under 25^oC.
Washing : PBST, 4 times, 10 minutes.

All lanes:

Western blot - Anti-ELOVL4 antibody (ab224608) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Lane 2:

Rat heart lysate

Lane 3:

Rat liver lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Elongation of very long chain fatty acids protein 4 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9GZR5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELOVL4 or Elongation of Very Long Chain Fatty Acids Protein 4 plays a critical role in the biosynthesis of very long chain fatty acids. It weighs approximately 32 kDa. It elongates saturated and polyunsaturated fatty acids extending carbon chains to lengths exceeding 24 carbons. ELOVL4 is found mainly in the retina skin and brain reflecting its significance in these tissues. Its mechanical function involves adding two carbons per cycle to endogenous and dietary fatty acids substrates.

Biological function summary

This enzyme contributes to the production of essential lipids that maintain cellular structure and function. ELOVL4 integrates into larger protein complexes that regulate its activity or stabilize its function. It influences the quality of long-chain ceramides and other sphingolipids which impact barrier functions particularly in the skin and retina. Moreover it participates in maintaining membrane integrity and dynamics where these specialized lipids concentrate.

Pathways

ELOVL4 participates prominently in sphingolipid and lipid metabolism pathways. It interacts with other elongases and desaturases such as ELOVL1 to execute its elongation tasks. This protein's activity bears importance in both the synthesis and modification phases of very long-chain fatty acids critical to cellular processes. The sphingolipid pathway necessitates ELOVL4 in producing functional lipids ensuring proper membrane properties and signaling events.

Mutations in the ELOVL4 gene have links to conditions like Stargardt-like macular dystrophy and ichthyosis. Stargardt disease involves retinal degeneration due to disruptions in photoreceptor cell function attributed to defective fatty acid elongation. In skin-related disorders like ichthyosis ELOVL4 mutations disturb lipid processing affecting skin barrier formation. Pathologically its dysfunction associates with other lipogenic and barrier-forming proteins including ceramide synthases impacting the synthesis of necessary lipid components.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that catalyzes the synthesis of very long chain saturated (VLC-SFA) and polyunsaturated (PUFA) fatty acids that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May play a critical role in early brain and skin development.

See full target information Elongation of very long chain fatty acids protein 4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 20:107 PubMed32989386

2020

Evaluation of immune-biomarker expression in high-grade soft-tissue sarcoma: HLA-DQA1 expression as a prognostic marker.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Yun Bae,Kyung Un Choi,Ahrong Kim,So Jung Lee,Kyungbin Kim,Jee Yeon Kim,In Sook Lee,So Hak Chung,Jeung Il Kim

Neurology. Genetics 6:e403 PubMed32211516

2020

Characterization of the phenotype with cognitive impairment and protein mislocalization in SCA34.

Applications

Unspecified application

Species

Unspecified reactive species

Marie Beaudin,Leila Sellami,Christian Martel,Lydia Touzel-Deschênes,Gabrielle Houle,Laurence Martineau,Kevin Lacroix,Andréane Lavallée,Nicolas Chrestian,Guy A Rouleau,François Gros-Louis,Robert Laforce,Nicolas Dupré

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ELOVL4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL4 antibody (AB224608)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL4 antibody (AB224608)

Immunocytochemistry/ Immunofluorescence - Anti-ELOVL4 antibody (AB224608)

Western blot - Anti-ELOVL4 antibody (AB224608)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

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