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Rabbit Recombinant Monoclonal ELOVL5 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

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Images

Western blot - Anti-ELOVL5 antibody [EPR17151] (AB205535), expandable thumbnail
  • Western blot - Anti-ELOVL5 antibody [EPR17151] (AB205535), expandable thumbnail
  • Western blot - Anti-ELOVL5 antibody [EPR17151] (AB205535), expandable thumbnail
  • Western blot - Anti-ELOVL5 antibody [EPR17151] (AB205535), expandable thumbnail
  • Western blot - Anti-ELOVL5 antibody [EPR17151] (AB205535), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C18:3(n-6) acyl-CoA. May participate in the production of monounsaturated and of polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators (By similarity) (PubMed:10970790, PubMed:20937905). In conditions where the essential linoleic and alpha linoleic fatty acids are lacking it is also involved in the synthesis of Mead acid from oleic acid (By similarity).

Alternative names

ELOVL2, PRO0530, ELOVL5, Very long chain fatty acid elongase 5, 3-keto acyl-CoA synthase ELOVL5, ELOVL fatty acid elongase 5, Elongation of very long chain fatty acids protein 5, Fatty acid elongase 1, Very long chain 3-ketoacyl-CoA synthase 5, Very long chain 3-oxoacyl-CoA synthase 5, ELOVL FA elongase 5, hELO1

Recommended products

Rabbit Recombinant Monoclonal ELOVL5 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17151
Purification technique
Affinity purification Protein A
Specificity

When testing sample with low protein level, we recommend uploading more amount of lysate, using lower antibody dilution, and using higher sensitivity ECL substrate. 

We also recommend trying 1%SDS Hot lysis prepare method to get desired Western Blot results.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ELOVL5 also known as elongation of very long chain fatty acids protein 5 is an important enzyme involved in the elongation process of fatty acids. It is part of a family that catalyzes the first and rate-limiting step of the elongation cycle adding two carbon units to the acyl chain. ELOVL5 is mainly found in tissues with high lipid metabolism including liver and brain with significant expression also noted in breast tissue. It has a molecular weight of approximately 33.5 kDa.

Biological function summary

ELOVL5 contributes to the metabolism of polyunsaturated fatty acids (PUFAs) which are important components of cell membranes and precursors to signaling molecules. It does not function alone but as part of a multi-enzyme complex that includes desaturases and reductases which enables the efficient synthesis of very long chain fatty acids (VLCFAs). These VLCFAs are critical for maintaining membrane fluidity and for cell signaling processes.

Pathways

ELOVL5 plays a significant role in the biosynthesis pathway of polyunsaturated fatty acids. It works alongside other enzymes such as desaturases to produce long-chain PUFAs from shorter precursor fatty acids. This process is important for the homeostasis of lipid signaling and membrane architecture. ELOVL5 also interacts with ELOVL6 another enzyme within the fatty acid elongation pathway indicating a coordinated interplay in fatty acid metabolism.

Associated diseases and disorders

Mutations or deficiencies in ELOVL5 can lead to conditions affecting lipid metabolism. One such disorder is spinocerebellar ataxia 38 (SCA38) a neurodegenerative disorder characterized by issues with movement and balance. Research also suggests a link between ELOVL5 and autism spectrum disorders (ASD) though this connection requires further investigation. In these disorders ELOVL5 often interacts with other proteins involved in lipid processing such as fatty acid synthase (FASN) underlying the complex nature of these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535), expandable thumbnail

    Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535) at 1/1000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Lane 3: Human fetal kidney lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 35 kDa

    Exposure time: 30s

  • Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535), expandable thumbnail

    Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535) at 1/1000 dilution

    All lanes: Human testis lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 35 kDa

    Observed band size: 35 kDa

    Exposure time: 3min

  • Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535), expandable thumbnail

    Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse heart lysate at 10 µg

    Lane 3: Mouse kidney lysate at 10 µg

    Lane 4: Mouse spleen lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Predicted band size: 35 kDa

    Observed band size: 35 kDa

    Exposure time: 3min

  • Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535), expandable thumbnail

    Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535) at 1/1000 dilution

    Lane 1: Rat brain lysate at 10 µg

    Lane 2: Rat heart lysate at 10 µg

    Lane 3: Rat kidney lysate at 10 µg

    Lane 4: Rat spleen lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Predicted band size: 35 kDa

    Observed band size: 35 kDa

    Exposure time: 15s

  • Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535), expandable thumbnail

    Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535) at 1/1000 dilution

    Lane 1: C6 (Rat glial tumor cells) whole cell lysate at 10 µg

    Lane 2: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Predicted band size: 35 kDa

    Observed band size: 35 kDa

    Exposure time: 30s

  • Immunocytochemistry/ Immunofluorescence - Anti-ELOVL5 antibody [EPR17151] (ab205535), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ELOVL5 antibody [EPR17151] (ab205535)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ELOVL5 with ab205535 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on HeLa cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab205535 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-ELOVL5 antibody [EPR17151] (ab205535), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ELOVL5 antibody [EPR17151] (ab205535)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling ELOVL5 with ab205535 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on HepG2 cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab205535 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-ELOVL5 antibody [EPR17151] (ab205535), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ELOVL5 antibody [EPR17151] (ab205535)

    Intracellular Flow Cytometry analysis ofHeLa cells labelling ELOVL5 with ab205535 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535), expandable thumbnail

    Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535)

    Blocking buffer and concentration: 5% NFDM/TBST. 

    Diluting buffer and concentration: 5% NFDM/TBST.

    When testing sample with low protein level, we recommend uploading more amount of lysate, using lower antibody dilution, and using higher sensitivity ECL substrate.    

    We also recommend trying 1%SDS Hot lysis prepare method to get desired Western Blot results.

    All lanes: Western blot - Anti-ELOVL5 antibody [EPR17151] (ab205535) at 1/1000 dilution

    Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method at 20 µg

    Lane 2: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method at 20 µg

    Lane 3: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method at 20 µg

    Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method at 20 µg

    Lane 5: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa

    Observed band size: 35 kDa

    Exposure time: 180s

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Product protocols

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