AB248617

Anti-Elp4 antibody [EPR4200(2)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ELP4 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

C11orf19, PAXNEB, ELP4, Elongator complex protein 4, hELP4, PAX6 neighbor gene protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Elp4 antibody [EPR4200(2)] - BSA and Azide free (AB248617)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Elp4 antibody [EPR4200(2)] - BSA and Azide free (AB248617)

This data was developed using ab133687, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labelling Elp4 with ab133687 at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elp4 antibody [EPR4200(2)] - BSA and Azide free (AB248617)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elp4 antibody [EPR4200(2)] - BSA and Azide free (AB248617)

This data was developed using ab133687, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labelling Elp4 with ab133687 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Elp4 antibody [EPR4200(2)] - BSA and Azide free (AB248617)

This data was developed using ab133687, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Elp4 antibody [EPR4200(2)] (<a href='/en-us/products/primary-antibodies/elp4-antibody-epr42002-ab133687'>ab133687</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

NIH/3T3 cell lysate at 10 µg

Lane 3:

Y79 cell lysate at 10 µg

Lane 4:

U87-MG cell lysate at 10 µg

Lane 5:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 46 kDa

false

Unconjugated

Anti-Elp4 antibody [EPR4200(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4200(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab248617 is the carrier-free version of ab133687.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Elp4 also known as Elongator complex protein 4 has an important role in the transcription process within cells. Its molecular weight is approximately 46 kDa. Elp4 is expressed in multiple tissues throughout the body with higher presence observed in the brain. It works as a component of the larger Elongator complex collaborating with other proteins such as Elp1 Elp2 Elp3 Elp5 and Elp6.

Biological function summary

Elongator complex proteins contribute to various cellular functions. Elp4 plays an important role in facilitating the modification of tRNA molecules necessary for efficient protein translation. It acts as part of the Elongator complex which participates in transcriptional elongation impacting processes like acetylation of histone 3 lysine 14. Elp4's activity in these processes ensures the proper progression and regulation of gene expression.

Pathways

Elp4 integrates into several significant cellular pathways. Notably it is active in the transcriptional elongation pathway an important process for proper gene expression. It also interacts with the oxidative stress response pathway aiding cells to manage stress. Within these pathways Elp4 collaborates closely with other elements of the Elongator complex ensuring synchronized functioning in transcription and stress response.

Research indicates that mutations or dysfunctions in Elp4 can contribute to several neurological conditions particularly juvenile-onset dystonia. This connection links Elp4 indirectly with other proteins involved in neural function such as dopamine receptor pathways. Additionally Elp4 is implicated in neurodevelopmental disorders like Rolandic epilepsy where its interaction with other components in the neuronal signaling may be disrupted.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (PubMed : 29332244). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (PubMed : 29332244).

See full target information ELP4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 10:e727 PubMed36301031

2022

Forkhead box M1 recruits FoxP3 Treg cells to induce immune escape in hilar cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ma,Zhaowei Sun,Xueliang Li,Jingyun Guo,Qinlei Wang,Mujian Teng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Elp4 antibody [EPR4200(2)] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Elp4 antibody [EPR4200(2)] - BSA and Azide free (AB248617)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elp4 antibody [EPR4200(2)] - BSA and Azide free (AB248617)

Western blot - Anti-Elp4 antibody [EPR4200(2)] - BSA and Azide free (AB248617)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise