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AB53540

Anti-ELYS antibody [2318C1a]

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(2 Publications)

Mouse Monoclonal ELYS antibody. Suitable for WB and reacts with Recombinant fragment samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AHCTF1.

View Alternative Names

ELYS, TMBS62, MSTP108, AHCTF1, Protein ELYS, Embryonic large molecule derived from yolk sac, Protein MEL-28, Putative AT-hook-containing transcription factor 1

1 Images
Western blot - Anti-ELYS antibody [2318C1a] (AB53540)
  • WB

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Western blot - Anti-ELYS antibody [2318C1a] (AB53540)

The molecular weight of the band on the Western blot does not correspond to the molecular weight of the natural protein as a recombinant protein was used as a test antigen

All lanes:

Western blot - Anti-ELYS antibody [2318C1a] (ab53540)

All lanes:

Immunized recombinant protein

Predicted band size: 252 kDa

Observed band size: 38 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2318C1a

Isotype

IgG1

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human AHCTF1. The exact immunogen used to generate this antibody is proprietary information.

Q8WYP5

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ELYS protein also known as MEL-28 is a critical component of the nuclear pore complexes (NPCs) and plays an important role in nuclear envelope assembly. ELYS is approximately 240 kDa in mass and is expressed in various tissues with significant presence in dividing cells. ELYS facilitates the recruitment of nucleoporins to chromatin which is necessary for the formation of NPCs during cell division.
Biological function summary

ELYS protein associates as part of the nuclear pore complex. It functions as a nucleoporin that mediates the interaction between chromatin and NPC components. This interaction is essential for the reassembly of the nuclear envelope and the reestablishment of nucleocytoplasmic transport after mitosis. ELYS acts through its AT-hook domains allowing it to bind to chromatin.

Pathways

ELYS integrates into mechanisms involving nuclear envelope reformation and nucleocytoplasmic transport pathways. It associates with key proteins in these pathways including NUP107-160 complex establishing a functional connection between chromatin and the forming NPCs. ELYS is essential for the correct localization of other nucleoporins ensuring efficient nuclear pore assembly and nuclear transport processes.

ELYS protein's dysfunction has links to cancer and neurological disorders. Defects in ELYS expression or mutations can impact correct NPC assembly affecting cellular homeostasis and leading to tumorigenesis. ELYS interaction with protein p53 plays an essential role in maintaining genomic stability which when disrupted can contribute to cancer development. Additionally changes in ELYS function have implications in neurodegenerative diseases where nuclear transport becomes compromised.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis.
See full target information AHCTF1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 6: PubMed36849252

2023

Three-dimensional growth reveals fine-tuning of 5-lipoxygenase by proliferative pathways in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara Göbel,Bjarne Goebel,Marius Hyprath,Ira Lamminger,Hannah Weisser,Carlo Angioni,Marius Mathes,Dominique Thomas,Astrid S Kahnt

eLife 7: PubMed30084827

2018

Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa Kane,Stephanie V Rebensburg,Matthew A Takata,Trinity M Zang,Masahiro Yamashita,Mamuka Kvaratskhelia,Paul D Bieniasz
View all publications
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