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AB14431

Anti-ELYS antibody

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(2 Publications)

Rabbit Polyclonal ELYS antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AHCTF1.

View Alternative Names

ELYS, TMBS62, MSTP108, AHCTF1, Protein ELYS, Embryonic large molecule derived from yolk sac, Protein MEL-28, Putative AT-hook-containing transcription factor 1

1 Images
Western blot - Anti-ELYS antibody (AB14431)
  • WB

Unknown

Western blot - Anti-ELYS antibody (AB14431)

ab14431 at 0.5mg/ml staining human ELYS by Western blot using chemiluminescence with exposure times of 3-5 minutes. Left lane 10mg HeLa nuclear extract, right lane 20mg.

All lanes:

Western blot - Anti-ELYS antibody (ab14431) at 0.5 µg/mL

Lane 1:

Cell lysates prepared from Hela cells at 10 µg/mL

Lane 2:

Cell lysates prepared from Hela cells at 20 µg/mL

Predicted band size: 252 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human AHCTF1. The exact immunogen used to generate this antibody is proprietary information.

Q8WYP5

Specificity

We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was purified using an epitope specific to ELYS immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 8% PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ELYS protein also known as MEL-28 is a critical component of the nuclear pore complexes (NPCs) and plays an important role in nuclear envelope assembly. ELYS is approximately 240 kDa in mass and is expressed in various tissues with significant presence in dividing cells. ELYS facilitates the recruitment of nucleoporins to chromatin which is necessary for the formation of NPCs during cell division.
Biological function summary

ELYS protein associates as part of the nuclear pore complex. It functions as a nucleoporin that mediates the interaction between chromatin and NPC components. This interaction is essential for the reassembly of the nuclear envelope and the reestablishment of nucleocytoplasmic transport after mitosis. ELYS acts through its AT-hook domains allowing it to bind to chromatin.

Pathways

ELYS integrates into mechanisms involving nuclear envelope reformation and nucleocytoplasmic transport pathways. It associates with key proteins in these pathways including NUP107-160 complex establishing a functional connection between chromatin and the forming NPCs. ELYS is essential for the correct localization of other nucleoporins ensuring efficient nuclear pore assembly and nuclear transport processes.

ELYS protein's dysfunction has links to cancer and neurological disorders. Defects in ELYS expression or mutations can impact correct NPC assembly affecting cellular homeostasis and leading to tumorigenesis. ELYS interaction with protein p53 plays an essential role in maintaining genomic stability which when disrupted can contribute to cancer development. Additionally changes in ELYS function have implications in neurodegenerative diseases where nuclear transport becomes compromised.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis.
See full target information AHCTF1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 24:1584-1594 PubMed36302971

2022

The chaperone DNAJB6 surveils FG-nucleoporins and is required for interphase nuclear pore complex biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

E F Elsiena Kuiper,Paola Gallardo,Tessa Bergsma,Muriel Mari,Maiara Kolbe Musskopf,Jeroen Kuipers,Ben N G Giepmans,Anton Steen,Harm H Kampinga,Liesbeth M Veenhoff,Steven Bergink

Scientific reports 9:8461 PubMed31186495

2019

Nuclear formation induced by DNA-conjugated beads in living fertilised mouse egg.

Applications

Unspecified application

Species

Unspecified reactive species

Yuka Suzuki,Şükriye Bilir,Yu Hatano,Tatsuhito Fukuda,Daisuke Mashiko,Shouhei Kobayashi,Yasushi Hiraoka,Tokuko Haraguchi,Kazuo Yamagata
View all publications
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